Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis

Almeida-Val, Vera Maria Fonseca de; Oliveira, Alice Reis; Silva, Maria de Nazaré Paula da; Ferreira-Nozawa, Monica S.; Araújo, Roziete Mendes; Val, Adalberto Luís; Nozawa, Shiro

doi:10.1590/s1415-47572011000200025

Cited by 28 publications

(15 citation statements)

References 30 publications

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“…Increased use of anaerobic glycolysis is a common response of fish to hypoxia (Bickler and Buck, 2007;Richards, 2009;Routley et al, 2002;Virani and Rees, 2000;Vornanen et al, 2009). Increases in flux through anaerobic pathways can occur rapidly (Richards et al, 2008;van Ginneken et al, 1995;Wallimann et al, 1992) and can then be reinforced over time by modifications in gene expression and/or enzyme activities (Almeida-Val et al, 2011;Greaney et al, 1980;Martinez et al, 2006). However, chronic use of anaerobic metabolism is unsustainable in most species due to the depletion of carbohydrate fuel stores, the accumulation of end products (e.g.…”

Section: Introductionmentioning

confidence: 99%

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish (Fundulus heteroclitus)

Borowiec

McClelland

Rees

et al. 2018

Journal of Experimental Biology

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Many fish experience daily cycles of hypoxia in the wild, but the physiological strategies for coping with intermittent hypoxia are poorly understood. We examined how killifish adjust O 2 supply and demand during acute hypoxia, and how these responses are altered after prolonged acclimation to constant or intermittent patterns of hypoxia exposure. We acclimated killifish to normoxia (∼20 kPa O 2), constant hypoxia (2 kPa) or intermittent cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28 days, and then compared whole-animal O 2 consumption rates (Ṁ O2) and tissue metabolites during exposure to 12 h of hypoxia followed by reoxygenation in normoxia. Normoxiaacclimated fish experienced a pronounced 27% drop in Ṁ O2 during acute hypoxia, and modestly increased Ṁ O2 upon reoxygenation. They strongly recruited anaerobic metabolism during acute hypoxia, indicated by lactate accumulation in plasma, muscle, liver, brain, heart and digestive tract, as well as a transient drop in intracellular pH, and they increased hypoxia inducible factor (HIF)-1α protein abundance in muscle. Glycogen, glucose and glucose-6-phosphate levels suggested that glycogen supported brain metabolism in hypoxia, while the muscle used circulating glucose. Acclimation to constant hypoxia caused a stable ∼50% decrease in Ṁ O2 that persisted after reoxygenation, with minimal recruitment of anaerobic metabolism, suggestive of metabolic depression. By contrast, fish acclimated to intermittent hypoxia maintained sufficient O 2 transport to support normoxic Ṁ O2 , modestly recruited lactate metabolism and increased Ṁ O2 dramatically upon reoxygenation. Both groups of hypoxia-acclimated fish had similar glycogen, ATP, intracellular pH and HIF-1α levels as normoxic controls. We conclude that different patterns of hypoxia exposure favour distinct strategies for matching O 2 supply and O 2 demand.

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Section: Introductionmentioning

confidence: 99%

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish (Fundulus heteroclitus)

Borowiec

McClelland

Rees

et al. 2018

Journal of Experimental Biology

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“…In the present study, we have found that muscle LDH mRNA levels were somewhat similar to that of the normoxic group following both short-and long-term exposures. In accordance, no changes in the expression of LDH-A were detected in muscle of either the hypoxia-tolerant killifish after exposure to 15 h of severe hypoxia (Richards et al 2008) or adults Oscar fish, A. crassipinis after hypoxia or anoxia (Almeida-Val et al 2011). This suggests that skeletal muscles have developed other mechanisms of tolerance and do not depend exclusively on gene regulation to survive hypoxic episodes.…”

Section: Discussionmentioning

confidence: 52%

“…In an extensive review, Nikinmaa (2002) suggested that the possible oxygen tensions leading to the stabilization of HIF-1 protein have been insufficiently studied and that they may vary between species and cell types. If this is true, the stabilization or decrease in LDH-A expression could be explained as a result of this process (Almeida-Val et al 2011). Furthermore, muscle-type tissues have an already high glycolytic capacity that may be more than adequate to match the reduced metabolic demands of hypoxia, which was suggested by Gracey et al (2001) to explain the difference in the tissue expression of glycolytic enzymes.…”

Section: Discussionmentioning

confidence: 98%

Metabolic and molecular responses in Nile tilapia,Oreochromis niloticusduring short and prolonged hypoxia

Mahfouz

Hegazi

El-Magd

et al. 2015

Marine and Freshwater Behaviour and Physiology

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The strictly aquatic breathing Nile tilapia, Oreochromis niloticus is an extremely hypoxia-tolerant fish. To augment our understanding of the effects of hypoxia on anaerobic glycolysis in the Nile tilapia, we studied the effect of short-term for 1 day (trial 1) and long-term for 30 days (trial 2) hypoxia on a selected glycolytic enzymes activity and mRNA expression in liver and white muscle. The hypoxic oxygen concentrations used in the two trials were 2, 1, and 0.5 mg O 2 L −1 for comparison with a control normoxic group 8 mg O 2 L −1 . The activity of phosphofructokinase (PFK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) in liver and white muscle except liver LDH decreased in trial 1 and increased in trial 2. Assessments of mRNA levels in trial 1 revealed that PFK was downregulated and LDH was upregulated in liver and white muscle, while PK fluctuated between upregulation in liver and downregulation in white muscle. Meanwhile, PK and LDH were upregulated while PFK was similar to control values in both tissues in trial 2. Comet assay results demonstrated an increase in DNA damage that was directly proportional to increasing hypoxic concentrations. This damage was more pronounced in trial 1. This suggests that the Nile tilapia cope better with long-term hypoxic conditions, possibly as an adaptive response.

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“…Manuscript to be reviewed mitochondria. However, LDH can reconvert lactate to pyruvate, when oxygen is available again (Hochachka and Somero, 1984;Wieser et al, 1987;Almeida-Val et al, 2011). The higher LDH activity in red muscles could be useful to deal with the higher lactate levels after the exercise, as observed for matrinchã.…”

Section: Are the Anaerobic Metabolic Adjustments Different Between Thmentioning

confidence: 99%

Peer Review #2 of "Could resistance to lactate accumulation contribute to the better swimming performance of Brycon amazonicus when compared to Colossoma macropomum? (v0.1)"

2018

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Background. In the wild, matrinchã (Brycon amazonicus) and tambaqui (Colossoma macropomum) rely strongly on their swimming capacity to perform feeding, migration and reproductive activities. Sustained swimming speed in fishes is performed almost exclusively by aerobic red muscles. The white muscle has high contraction power, but fatigue quickly, being used mainly in sprints and bursts, with a maximum duration of few seconds. The Ucrit test, an incremental velocity procedure, is mainly a measure of the aerobic capacity of a fish, but with a high participation of anaerobic metabolism close to the velocity of fatigue. Our previous study has indicated a high swimming performance of matrinchã (Ucrit) after hypoxia exposure, despite increased levels of lactate in plasma. In contrast, tambaqui with high lactate levels in plasma presented very low swimming performance. Therefore, we aimed to study the resistance of matrinchã and tambaqui to the increased lactate levels in muscle over an incremental velocity test (Ucrit). As a secondary aim, we analyzed the differences in anaerobic metabolism in response to environmental hypoxia, which could also support the better swimming performance of matrinchã, compared to tambaqui. Methods. We measured, over incremented velocities in both species, the metabolic rate (the oxygen consumption by the fish; MO 2), and the concentrations of lactate and nitrites and nitrates (NOx) in muscles. NOx was measured as an indicator of nitric oxide and its possible role in improving cardiorespiratory capacity in these fishes, which could postpone the use of anaerobic metabolism and lactate production during the swimming test. Also, we submitted fishes until fatigue and hypoxia (0.5 mg L-1) and measured, in addition to the previous parameters, lactate dehydrogenase activity (LDH; the enzyme responsible for lactate production), since that swimming performance could also be explained by the anaerobic capacity of producing ATP. Results. Matrinchã exhibited a better swimming performance and higher oxygen consumption rates. Lactate levels were higher in matrinchã only at the moment of fatigue. Under hypoxia, LDH activity increased in the white muscle only in tambaqui, but averages were always higher in matrinchã. Discussion and conclusions. The results suggest that matrinchã is more resistant than tambaqui regarding lactate accumulation in muscle at the Ucrit test, but it is not clear how much it contributes to postpone fatigue. The higher metabolic rate possibly allows the accumulated lactate to be used as aerobic fuel by the matrinchã, improving swimming performance. More studies are needed regarding matrinchã's ability to oxidize lactate, the effects of exercise on muscle acidification, and the hydrodynamics of these species, to clarify why matrinchã is a better swimmer than tambaqui.

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Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis

Cited by 28 publications

References 30 publications

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish (Fundulus heteroclitus)

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish (Fundulus heteroclitus)

Metabolic and molecular responses in Nile tilapia,Oreochromis niloticusduring short and prolonged hypoxia

Peer Review #2 of "Could resistance to lactate accumulation contribute to the better swimming performance of Brycon amazonicus when compared to Colossoma macropomum? (v0.1)"

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