Genetic characterization of 18 novel microsatellite loci in northern pike (Esox lucius L.)

Genetic introgression from stocked adult northern pike Esox lucius to a wild self‐recruiting population was detected in a large river system and some stocked E. lucius survived up to two spawning seasons and dispersed over several kilometres in the river. Moreover, the catch rate of stocked E. lucius by anglers was low (9.6%), hence suggesting that the efficiency of stocking activity is questionable.

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“…), Eluc‐033, Eluc‐040 (Wang et al . ), EluBe‐INRA, EluB‐38INRA, EluC‐113INRA (Launey et al . ), were amplified using three multiplexed PCRs.…”

Section: Results Of the Generalized Linear Model Aimed At Testing Thementioning

confidence: 99%

Catch‐related and genetic outcome of adult northern pike Esox lucius stocking in a large river system

Guillerault

Loot

Blanchet

et al. 2018

Journal of Fish Biology

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“…Six of these loci proved to be variable during screening. A total of 752 individuals were successfully amplified and genotyped at the six microsatellite loci: Elu19 (Miller & Kapuscinski, 1997), Eluc004 and Eluc045 (Aguilar et al ., 2005), B118INRA (Launey et al ., 2003), B24 and B451 (Wang et al ., 2011). Loci were amplified in a single 10 μL multiplex polymerase chain reaction (PCR) containing 1 μL of DNA (25 ng/μL), 5 μL Multiplex PCR Mastermix (Qiagen, Crawley, UK) and labelled primers (FAM, VIC, NED and PET) with the following concentrations: Elu 19 (0.25 μ m ), Eluc004 (0.4 μ m ), Eluc045 (0.4 μ m ), B118INRA (0.25 μ m ), B24 (0.4 μ m ) and B451 (0.25 μ m ).…”

Section: Methodsmentioning

confidence: 99%

Genetic structure of pike (Esox lucius) reveals a complex and previously unrecognized colonization history of Ireland

Pedreschi

Kelly‐Quinn

Caffrey

et al. 2013

Journal of Biogeography

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AimWe investigated genetic variation of Irish pike populations and their relationship with European outgroups, in order to elucidate the origin of this species to the island, which is largely assumed to have occurred as a human-mediated introduction over the past few hundred years. We aimed thereby to provide new insights into population structure to improve fisheries and biodiversity management in Irish freshwaters.LocationIreland, Britain and continental Europe.MethodsA total of 752 pike (Esox lucius) were sampled from 15 locations around Ireland, and 9 continental European sites, and genotyped at six polymorphic microsatellite loci. Patterns and mechanisms of population genetic structure were assessed through a diverse array of methods, including Bayesian clustering, hierarchical analysis of molecular variance, and approximate Bayesian computation.ResultsVarying levels of genetic diversity and a high degree of population genetic differentiation were detected. Clear substructure within Ireland was identified, with two main groups being evident. One of the Irish populations showed high similarity with British populations. The other, more widespread, Irish strain did not group with any European population examined. Approximate Bayesian computation suggested that this widespread Irish strain is older, and may have colonized Ireland independently of humans.Main conclusionsPopulation genetic substructure in Irish pike is high and comparable to the levels observed elsewhere in Europe. A comparison of evolutionary scenarios upholds the possibility that pike may have colonized Ireland in two ‘waves’, the first of which, being independent of human colonization, would represent the first evidence for natural colonization of a non-anadromous freshwater fish to the island of Ireland. Although further investigations using comprehensive genomic techniques will be necessary to confirm this, the present results warrant a reappraisal of current management strategies for this species.

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“… High‐resolution microsatellites selected according to Eschbach and Schöning (2013) for population genetic analysis of species with low genetic variability. References: 1 = Miller & Kapuscinski, 1996; 2 = Wang et al., 2011; 3 = Aguilar et al., 2005; 4 = Wildlife Forensics Laboratory, California, USA (internal report); 5 = Miller & Kapuscinski, 1997; 6 = Ouellet‐Cauchon et al., 2014; 7 = Sloss et al., 2008; 8 = Launey et al, 2003. NED, FAM, and HEX are fluorescent dyes; A T = total number of different alleles over all populations, A M = mean number of different alleles per population, H O = observed heterozygosity, H S = heterozygosity according to Nei & Chesser (1983).…”

Section: Methodsmentioning

confidence: 99%

Genetic population structure of a top predatory fish (northern pike, Esox lucius) covaries with anthropogenic alteration of freshwater ecosystems

et al. 2021

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Esox lucius L. (northern pike) inhabits river, lake, and brackish water ecosystems of the temperate region in North America and Eurasia. In many areas of its native range, the species is confronted with human‐induced disturbances that can affect local and regional genetic diversity. Conservation of genetic diversity constitutes an important policy objective. Therefore, we investigated how the genetic population structure and patterns suggestive of hybridisation among subpopulations from different catchments vary with the type of ecosystem (river vs. lake) and the integrity of the ecosystem assessed using ecological status assessments of the European Water Framework Directive and indices of the wetland quality (in rivers) and trophic state (in lakes). In total, 1,384 pike samples from the North, Baltic, and Black Sea drainages in Germany were analysed. We differentiated between metapopulations from each hydrogeographic region using genotypes from 15 microsatellites and mitochondrial cyt b sequences. Individual populations showed signs of genetic admixture ranging from almost zero to complete replacement by foreign genotypes originating from different catchments. Hierarchical general linear modelling revealed a significantly positive association of the degree of genetic hybridisation with decreasing ecological status. An eroded population substructure indicative of hybridisation was also significantly elevated in rivers relative to lakes and in heavily modified water bodies. Results were consistent when using ecological status assessments by the Water Framework Directive and an alternative environmental quality index based on the health of wetlands in rivers and trophic state in lakes. We speculate that macrophyte loss through loss of wetlands or in response to eutrophication fostered genetic mixing among different subpopulations, which were brought into secondary contact either due to stocking or due to natural dispersal from foreign catchments. Our findings suggest that anthropogenic alteration of freshwater ecosystems may influence the genetic structures present in a freshwater top piscivore at both local and regional scales.

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Genetic characterization of 18 novel microsatellite loci in northern pike (Esox lucius L.)

Cited by 7 publications

References 10 publications

Catch‐related and genetic outcome of adult northern pike Esox lucius stocking in a large river system

Catch‐related and genetic outcome of adult northern pike Esox lucius stocking in a large river system

Genetic structure of pike (Esox lucius) reveals a complex and previously unrecognized colonization history of Ireland

Genetic population structure of a top predatory fish (northern pike, Esox lucius) covaries with anthropogenic alteration of freshwater ecosystems

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