The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds

Zhang, Chunyan; Liu, Yun; Huang, Kai; Zeng, Wenbing; Xu, Deqing; Wen, Qunying; Yang, Liguo

doi:10.1590/s1415-47572010005000110

Cited by 45 publications

(34 citation statements)

References 32 publications

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“…Similarly, AG of A815G revealed low body weight. AA of A1753 insertion revealed heavy body weight in both the breeds and 7% longer Hua et al (2009) and Zhang et al (2011), where the presence of two genotypes; one of them is homozygous with G/G nucleotide and the other is heterozygous with A/G nucleotide in tested animals and the absence of homozygous genotype with A/A nucleotide and also the presence of heterozygous genotype with the highest frequencies in goat breeds. In Egyptian animals, the frequencies of AG heterozygous genotype were 56.44 and 87.5% for sheep and goat animals, respectively.…”

Section: Resultsmentioning

confidence: 90%

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Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Othman¹,

Alam²,

El-Kader³

et al. 2015

Biotechnology

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A B S T R A C T Small ruminants are considered as one of the major sources of meat and milk production in Egypt. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. In this study, we aimed to detect the genetic polymorphism of GH gene in major Egyptian sheep and goat breeds using PCR-RFLP and identify the single nucleotide polymorphism between different genotypes detected in these breeds. The primers used in this study flanked a 422 bp fragment from exons 2 and 3 of GH gene in sheep and goat. These PCR amplified fragments were digested with HaeIII endonuclease and the results showed the presence of two genotypes; GG and AG with the absence of AA genotype in 149 tested animals for this gene. The total frequencies were 43.56 and 56.44% for GG and AG genotypes, respectively in 101 tested sheep animals whereas in goat animals, the total frequencies were 12.5 and 87.5% for GG and AG genotypes, respectively in 48 tested goat animals. The sequence analysis of purified PCR products representing these two detected genotypes declared the presence of a SNP (G6A) at position 55 in the amplified fragment which is responsible for the destruction of the restriction site GG^CC and consequently the presence of two different alleles G and A which were named in this work according to the detected SNP. The nucleotide sequences of sheep GH alleles G and A as well as goat GH alleles G and A were submitted to nucleotide sequences database NCBI/Bankit/GenBank and have accession numbers: KP120857, KP120858, KP120859 and KP120860, respectively. In conclusion, production improvements can be achieved by using new genetic technology for better selection of heritable traits through marker-assisted selection. Due to the reported association between genotype possess A/G nucleotide with different growth trait parameters, we recommend to increase this heterozygous genotype in native small ruminant breeds through the selection of animals have AG genotype of GH gene and enter them in breeding programs of Egyptian small ruminants as a way to increase their production traits.

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Section: Resultsmentioning

confidence: 90%

“…On the other hand, these two active mutations (A781G and A1575G) in growth hormone gene and their associations with litter size were investigated in both high and low prolificacy goat breeds by Zhang et al (2011). Two genotypes in each mutation were detected in these two goat breeds.…”

Section: Resultsmentioning

confidence: 99%

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Othman¹,

Alam²,

El-Kader³

et al. 2015

Biotechnology

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“…It seems that a study on major genes known for the twining trait can distinguish S subpopulations from T subpopulations. For example, Rasouli et al (2016) reported that the twinning rate in Markhoz goats was significantly influenced by the IGF-I and IGFBP-3 genotypes; also, the association of two single nucleotide polymorphisms (SNPs) in the growth hormone (GH) gene with the litter size and superovulation response in goat breeds has been studied, and the results have shown that the two loci of the GH gene are highly associated with abundant prolificacy and a superovulation response in goat breeds (Zhang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

et al. 2016

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Abstract. The aim of this study was to investigate the genetic diversity and structure in three Iranian native goat breeds (Markhoz, Mahabadi and Lori) and the Beetal imported breed using inter-simple sequence repeat (ISSR) markers and also to investigate ISSR markers' potential in order to genetically separate single (S) and twin-birth (T) subpopulations. Blood samples were collected from 210 animals for this purpose. In total, 16 primers were used, and finally 5 primers were selected based on the number of clear bands and the level of polymorphisms. The result of this study showed that 76 of 86 observed fragments were polymorphic. Genetic diversity for each breed ranged from 0.23 in the Beetal breed to 0.26 in the Markhoz breed; this represents a relatively similar genetic diversity in these breeds. An unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the Nei's standard genetic distance between the breeds studied showed that three Iranian goat breeds (Mahabadi, Lori and Markhoz) were clustered closer together, while the Beetal breed formed a separate cluster. In the constructed dendrogram of the subpopulations, the S and T subpopulations of each breed were clustered together. The constructed dendrogram of the Beetal breed and the S and T subpopulations of all breeds studied showed a separate cluster for the Beetal breed as an imported breed and another cluster for the S and T subpopulations as Iranian native breeds. The current study showed that the ISSR markers studied had no potential to genetically separate S and T subpopulations. On the other hand, these ISSR markers can be used for the clustering of distinct populations.

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“…Identification of direct markers is more useful than indirect markers for predicting the phenotypic superiority of the targeted traits of a population (DekkerS, 2004;WILLIaMS, 2005;ZhaNG et al, 2011). In animals, growth is regulated by a complex system, in which the somatotropic axis plays an important role (BUYSe and DeCUYPere, 1999).…”

Section: Introductionmentioning

confidence: 99%

Association of a novel single nucleotide polymorphism at the exon‑2 of Insulin-Like Growth Factor 1 (IGF1) gene with phenotypic variants in goats

Thomas¹,

Venkatachalapathy²,

Aravindakshan³

et al. 2017

Vet. arhiv

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ABSTRACTthe Insulin-Like Growth Factor 1 plays a key role in foetal development and post natal growth. the objectives of this study were to characterise the complete coding sequence of caprine IGF1 gene in two indigenous goat breeds of India: Malabari and attappady Black, to detect polymorphisms of IGF1 gene, to investigate their effects on body size traits and to ascertain the relative expression of IGF1 mrNa in muscle tissues of goats belonging to low and high body weight groups. All the four exons of caprine IGF1 gene were amplified and characterized by PCr-SSCP in 298 goats, revealing two genotypes (CC and Ct) at exon 2. Sequencing of the PCr products from each genotype revealed a novel SNP, g.80C>t (GenBank accession No. kM974180), which caused a non-synonymous mutation (thr48Met),causing differences in IGF1 protein structure. association analysis of the loci indicated Ct genotypes have higher body length (P<0.01), chest circumference (P<0.01) and body length index (P<0.05) than CC genotypes. two novel PCr-rFLPs were designed for the rapid detection of the genotypes. the quantitative real time PCr demonstrated a difference in the expression of IGF1 mrNa in muscle tissues of the low and high body weight groups, but it was not significant (P>0.05). The results of the *Corresponding author: Dr. Naicy thomas, M.V.Sc., PhD, assis. Prof., Department of animal Breeding, Genetics and Biostatistics, College of Veterinary and animal Sciences, Mannuthy, thrissur-680651, kerala, India, Phone: +91 94 4611 9307; e-mail: naicy@kvasu.ac.in 458Vet. arhiv 87 (4), [457][458][459][460][461][462][463][464][465][466][467][468][469][470][471][472] 2017 t. Naicy et al.: association of IGF1 gene polymorphism with phenotypic variants in goats present study suggest that the alleles of the IGF1 gene could be considered as strong targets for improvement of growth traits in goats.

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The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds

Cited by 45 publications

References 32 publications

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Genotyping of Growth Hormone Gene in Egyptian Small Ruminant Breeds

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

Association of a novel single nucleotide polymorphism at the exon‑2 of Insulin-Like Growth Factor 1 (IGF1) gene with phenotypic variants in goats

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