Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)

Neto, Maressa Ferreira; Vicari, Marcelo Ricardo; Camargo, Edemar Furquim de; Artoni, Roberto Ferreira; Moreira‐Filho, Orlando

doi:10.1590/s1415-47572009005000078

Cited by 22 publications

(31 citation statements)

References 18 publications

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“…The presence of 50 chromosomes in A. fasciatus was verified by Artoni et al (2006) in the Tibagi River, an upper Paraná River affluent. As in several studies about A. altiparanae (Jim & Toledo 1975;Pacheco et al 2001;Domingues et al 2007;Hashimoto et al 2008;Ferreira Neto et al 2009), the presence of 50 chromosomes was verified in the present study. Souza & Moreira-Filho (1995) stated that the number of chromosomes carrying NORs also denotes an important factor for the differentiation among the populations.…”

Section: Discussionsupporting

confidence: 90%

“…The presence of multiple NORs with interpopulational variation is common between the species of A. scabripinnis and A. fasciatus (Ferro et al 2001;Medrado et al 2008), as verified by the present study in A. paranae and A. fasciatus, presenting interpopulational differences in the location and in the number of chromosomes that carrying NORs. Variations in the NORs in A. altiparanae also have been observed (Fernandes & Martins-Santos 2004;Ferreira Neto et al 2009); however, in the present study, both populations presented the same pairs marked by the silver nitrate.…”

Section: Discussionsupporting

confidence: 64%

“…However, in A. altiparanae, there is a stability of the diploid number, with 50 chromosomes (Jim & Toledo 1975;Pacheco et al 2001). Despite this trend toward conservativeness in the diploid number among the population of the various streams within the same basin, variations in the karyotypic formula, number and location of the NORs were verified (Ferreira Neto et al 2009) and small variations of the distribution of the heterochromatin (Fernandes & Martins-Santos 2004).…”

Section: Introductionmentioning

confidence: 97%

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Interpopulational comparative cytogenetics analysis among three Astyanax (Characiformes: Incertae Sedis) species of two streams of upper Paraná River basin, Brazil

2014

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Populations of Astyanax paranae, Astyanax fasciatus and Astyanax altiparanae collected from the Pindorama and Lopei streams (upper Paraná river basin, Brazil) were cytogenetically studied, checking for chromosomal differentiation and the evolutionary trend in the three species. Forty-eight chromosomes (8m + 20sm + 10st + 10a) were observed in both populations of Astyanax paranae. Astyanax fasciatus exhibited 50 chromosomes (8m + 18sm + 10st + 14a, Pindorama stream; 8m + 14sm + 12st + 16a, Lopei stream). The diploid number for A. altiparanae was 50 chromosomes in both populations (6m + 30sm + 4st + 10a). The three studied species exhibited multiple AgNORs with differences among the populations of A. paranae and A. fasciatus. Regarding the heterochromatin distribution, interpopulational differences were observed in the three species. The data of the present study demonstrate that both A. paranae and A. fasciatus exhibit karyotypical interpopulational differences more conspicuous than A. altiparanae populations, probably due to the characteristics that restricted both the A. paranae and the A. fasciatus to the upper portion of the streams, leading to the fixation of different chromosomal rearrangements in the populations. However, the absence of conspicuos interpopulational karyotypical differences in A. altiparanae suggests a gene flow between both populations. In the present study, the role of the streams as an environment which maintains the diversity of fish with different genetic pools became evident.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 97%

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Interpopulational comparative cytogenetics analysis among three Astyanax (Characiformes: Incertae Sedis) species of two streams of upper Paraná River basin, Brazil

2014

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“…While the "scabripinnis complex" has been characterized by a preferential centromeric and telomeric pattern of C-bands (Fernandes and Martins-Santos, 2005;Souza et al, 2007;Santos et al, 2012), the "bimaculatus group" has shown mainly interstitial heterochromatic blocks on the chromosomes (Domingues et al, 2007;Hashimoto et al, 2008;Ferreira Neto et al, 2009;Kavalco et al, 2011). The species now analyzed show that the distribution of heterochromatin falls, in general, among the above cases.…”

Section: Discussionmentioning

confidence: 89%

“…bimaculatus showed the same diploid number (2n=50) but with differences in the karyotype formulas, probably due to rearrangements such as pericentric inversions. This last group, unlike the "scabripinnis group", has shown a constancy in the diploid number (Domingues et al, 2007;Pamponet et al, 2008;Ferreira Neto et al, 2009;Kavalco et al, 2011;Pacheco et al, 2011;Peres et al, 2011;Martinez et al, 2012), indicating an evolutionary karyotype pattern relatively more conserved than that of the "scabripinnis complex". In turn, the distinct karyotypes presented by the two populations of A. asuncionensis also show the occurrence of two karyomorphs and a probable "asuncionensis complex."…”

Section: Discussionmentioning

confidence: 99%

Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

et al. 2016

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The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the "scabripinnis complex", Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed". Two sympatric karyomorphs were observed in Astyanax. aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter-and intraspecific chromosomal diversity in the genus Astyanax.Keywords: cytogenetics, biodiversity, B chromosomes, C-bands, NORs. Diversidade Cariotípica entre três espécies do gênero

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Chromosomal characterization in two species of an Astyanax bimaculatus complex (Characidae, Characiformes) using different techniques of chromosome banding

et al. 2015

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Astyanax has been the subject of extensive cytogenetic studies due to its wide karyotypic diversity. This genus comprises species complexes, namely groups of fish of difficult morphological differentiation, such as the bimaculatus complex, which includes the characids with a rounded humeral spot. Thence, the present study proposed to accomplish a cytogenetic characterization of two species of this complex: A. asuncionensis and A. altiparanae, aiming to find chromosomal markers that differentiate these species, as well as achieve a better understanding of the karyotype evolution in the genus. For this we used different techniques of chromosome banding as C-banding, impregnation by silver nitrate, fluorochrome staining and FISH with 18S rDNA probe. This is the first cytogenetic study in A. asuncionensis, from Miranda river, which presented 2n = 50 and 18 m + 22sm + 6st + 4a (FN = 96) and single NORs. The populations of A. altiparanae also presented 2n = 50, but with different karyotypic formulae: the population of the Quexada river presented 16 m + 24sm + 4st + 6a (FN = 94) and the Esperança stream and Jacutinga river showed 16 m + 20sm + 4st + 10a (FN = 90). All analyzed populations showed an interindividual variation in the number and location of the nucleolar organizer regions (NORs). Single and multiple NORs were detected either by impregnation with silver nitrate or by FISH with 18S rDNA probe. After C-banding, the two species differed in relation to the composition and heterochromatin distribution. The meiotic cells of A. altiparanae male individuals were also analyzed, showing that, despite the high karyotype variability, chromosome pairing occurs normally. The data show that A. altiparanae and A. asuncionensis share some characteristics with other species of the bimaculatus complex, suggesting a close phylogenetic relationship among those species. However, some features can be used as differentiation chromosomal markers in altiparanae/asuncionensis morphotypes, which could result from a natural speciation process.

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Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)

Cited by 22 publications

References 18 publications

Interpopulational comparative cytogenetics analysis among three Astyanax (Characiformes: Incertae Sedis) species of two streams of upper Paraná River basin, Brazil

Interpopulational comparative cytogenetics analysis among three Astyanax (Characiformes: Incertae Sedis) species of two streams of upper Paraná River basin, Brazil

Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

Chromosomal characterization in two species of an Astyanax bimaculatus complex (Characidae, Characiformes) using different techniques of chromosome banding

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