Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster

Silva, Regildo Márcio Gonçalves da; Sousa, Neila Coelho de; Gräf, Ulrich; Spanó, Mário Antônio

doi:10.1590/s1415-47572008000400023

Cited by 7 publications

(2 citation statements)

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“…Previous studies have shown that DXR induces preferentially homologous recombination compared with mutational events in somatic cells of D. melanogaster (Lehmann et al , 2003; Fragiorge et al , 2007; Pereira et al , 2008; Silva et al , 2008; Valadares et al , 2008). …”

Section: Introductionmentioning

confidence: 99%

“…The anthracycline antibiotic doxorubicin is a topoisomerase II inhibitor ( Swift et al , 2008 ) and a generator of oxygen free radicals ( Doroshow, 1983 ). Previous studies have shown that DXR induces preferentially homologous recombination compared with mutational events in somatic cells of D. melanogaster ( Lehmann et al , 2003 ; Fragiorge et al , 2007 ; Pereira et al , 2008 ; Silva et al , 2008 ; Valadares et al , 2008 ).…”

Section: Introductionmentioning

confidence: 99%

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Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster

et al. 2009

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The wing Somatic Mutation and Recombination Test (SMART) in D. melanogaster was used to study genotoxicity of the medicinal plant Tabebuia impetiginosa. Lapachol (naphthoquinone) and β-lapachone (quinone) are the two main chemical constituents of T. impetiginosa. These compounds have several biological properties. They induce apoptosis by generating oxygen-reactive species, thereby inhibiting topoisomerases (I and II) or inducing other enzymes dependent on NAD(P)H:quinone oxidoreductase 1, thus affecting cell cycle checkpoints. The SMART was used in the standard (ST) version, which has normal levels of cytochrome P450 (CYP) enzymes, to check the direct action of this compound, and in the high bioactivation (HB) version, which has a high constitutive level of CYP enzymes, to check for indirect action in three different T. impetiginosa concentrations (10%, 20% or 40% w/w). It was observed that T. impetiginosa alone did not modify the spontaneous frequencies of mutant spots in either cross. The negative results observed prompted us to study this phytotherapeuticum in association with the reference mutagen doxorubicin (DXR). In co-treated series, T. impetiginosa was toxic in both crosses at higher concentration, whereas in the HB cross, it induced a considerable potentiating effect (from ~24.0 to ~95.0%) on DXR genotoxity. Therefore, further research is needed to determine the possible risks associated with the exposure of living organisms to this complex mixture.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster

et al. 2009

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Boswellic acid suppresses cyclophosphamide induced chromosomal damage in mice

Ganguly¹,

Jagadeesh²,

Singh³

et al. 2011

Orient Pharm Exp Med

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This study was performed to determine whether Boswellic acid suppresses genotoxicity induced by cyclophosphamide in mice. Genotoxicity was induced by the administration of cyclophosphamide (50 mg/kg) in swiss albino mice and was measured by counting the number of micronuclei and observing chromosomal aberration. In test group Boswellic acid was given in the dose of 200 mg/kg and various chromosomal aberration and formation of micronuclei were observed. Boswellic acid in dose of 200 mg/kg significantly (P<0.001) inhibited the cyclophosphamide induced micronucleus formation and also significantly (P<0.001) inhibited chromosomal aberrations in mice. Whereas Boswellic acid alone did not either induce formation of micronuclei or chromosomal aberration in mice. Boswellic acid itself doesn't have any genotoxic effect and suppresses genotoxicity induced by cyclophosphamide.

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A comparative study of the modulatory effects of (−)-cubebin on the mutagenicity/recombinogenicity induced by different chemical agents

Rezende

Munari

Oliveira

et al. 2013

Food and Chemical Toxicology

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(-)-Cubebin (CUB) is a lignan isolated from dry seeds of Piper cubeba. We aimed to assess its genotoxic potential and influence on chromosomal damage (frequency of micronuclei - MN) induced by doxorubicin (DXR) in V79 cells and by urethane (URE) in somatic Drosophila melanogaster cells. Our findings indicate an absence of a CUB-mediated genotoxic effect at the concentrations tested. The results also revealed that CUB significantly reduced the frequency of MN induced by DXR, with a mean reduction of 63.88%. In a previous study, our research group demonstrated an absence of CUB-mediated mutagenic effects through the wing Somatic Mutation and Recombination Test (SMART) in Drosophila. In the present study, we used the standard and high bioactivation versions of the SMART to estimate the antigenotoxic effects of CUB associated with URE. At lower concentrations, the recombination level decreased, but at the highest concentration, the recombination level increased. Our data and previous studies suggest that CUB may act as a free radical scavenger at low concentrations, a pro-oxidant at higher concentrations when it interacts with the enzymatic system that catalyzes the metabolic detoxification of DXR or URE, and/or an inducer of recombinational DNA repair.

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Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster

Cited by 7 publications

References 53 publications

Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster

Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster

Boswellic acid suppresses cyclophosphamide induced chromosomal damage in mice

A comparative study of the modulatory effects of (−)-cubebin on the mutagenicity/recombinogenicity induced by different chemical agents

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