2008
DOI: 10.1590/s1415-47572008000200038
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Abstract: License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cited by 3 publications
(3 citation statements)
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“…The genetic identification of hybrids is an important aspect of fish culture that helps to avoid problems associated with artificial hybridization ( Toledo-Filho et al , 1998 ; Porto-Foresti and Foresti, 2004 ). Among the currently available genetic methods, cytogenetics has contributed extensively to ichthyological studies and is an important tool for chromosomal, evolutionary and phylogenetic analyses and species identification ( Foresti, 2008 ; Oliveira et al , 2009 ). In hybridization studies, this technique is effective in assessing the levels of ploidy and in identifying gynogenetic and androgenetic lineages, the morphological and chromosomal structure of parental and hybrid species and lineages, structural alterations in the chromosomes of hybrids, and cytogenetic markers that allow discrimination between pure species and interspecific hybrid lineages ( Toledo-Filho et al , 1994 ; Almeida-Toledo et al , 1995 ; Porto-Foresti et al , 2008 ).…”
Section: Introductionmentioning
confidence: 99%
“…The genetic identification of hybrids is an important aspect of fish culture that helps to avoid problems associated with artificial hybridization ( Toledo-Filho et al , 1998 ; Porto-Foresti and Foresti, 2004 ). Among the currently available genetic methods, cytogenetics has contributed extensively to ichthyological studies and is an important tool for chromosomal, evolutionary and phylogenetic analyses and species identification ( Foresti, 2008 ; Oliveira et al , 2009 ). In hybridization studies, this technique is effective in assessing the levels of ploidy and in identifying gynogenetic and androgenetic lineages, the morphological and chromosomal structure of parental and hybrid species and lineages, structural alterations in the chromosomes of hybrids, and cytogenetic markers that allow discrimination between pure species and interspecific hybrid lineages ( Toledo-Filho et al , 1994 ; Almeida-Toledo et al , 1995 ; Porto-Foresti et al , 2008 ).…”
Section: Introductionmentioning
confidence: 99%
“…This technique often produced overlapping chromosomes, making it difficult to visualize the morphology and diploid number. Anyway, researches using this methodology continued and resulted in the creation of the "crushing machine" invented by Orlando Moreira Filho to minimize the injuries in the researcher's fingers [10].…”
Section: Classical Methods Of Cytogenetics To Obtain Metaphase Chromomentioning
confidence: 99%
“…Since 1956, the works of Tjio, Levan, Ford, and Hamerton [26,27] have reported about treating cytogenetic preparations with colchicine and hypotonic solutions, and the chromosomes have shown morphologically well-defined and that spread easily on the glass slide. Only in 1975, with the publication of a paper by McPhail and Jones, the chromosome preparations for cytogenetic studies in fish began to use this methodology [10].…”
Section: Cytogenetics -Past Present and Further Perspectivesmentioning
confidence: 99%