2007
DOI: 10.1590/s1415-47572007000400017
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Abstract: Retroelements are a diversified fraction of eukaryotic genomes, with the Ty1/copia and Ty3/gypsy groups being very common in a large number of plant genomes. We isolated an internal segment of the Ty3/gypsy retroelement of Cestrum strigilatum (Solanaceae) using PCR amplification with degenerate primers for a conserved region of reverse transcriptase. The isolated segment (pCs12) was sequenced and showed similarity with Ty3/gypsy retroelements of monocotyledons and dicotyledons. This segment was used as probe i… Show more

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Cited by 10 publications
(7 citation statements)
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“…FISH with the 45S rDNA probe did not show signals co-localized with pCa06 signals, except to C. racemosa . In relation to DAPI + bands we did observe co-location especially in C. arabica The strategy of isolating transposable elements using PCR with degenerate primers has been widely used to amplify conserved fragments of Ty1-copia-like or Ty3-gypsy-like retrotransposons in many plant species (Belyayev et al 2005;Fregonezi et al 2007;Gaeta et al 2010). The DOP-PCR procedure has been used to amplify different repetitive DNAs from entire genomes.…”
Section: Fish With 45s Rdna and Pca06 Probesmentioning
confidence: 77%
“…FISH with the 45S rDNA probe did not show signals co-localized with pCa06 signals, except to C. racemosa . In relation to DAPI + bands we did observe co-location especially in C. arabica The strategy of isolating transposable elements using PCR with degenerate primers has been widely used to amplify conserved fragments of Ty1-copia-like or Ty3-gypsy-like retrotransposons in many plant species (Belyayev et al 2005;Fregonezi et al 2007;Gaeta et al 2010). The DOP-PCR procedure has been used to amplify different repetitive DNAs from entire genomes.…”
Section: Fish With 45s Rdna and Pca06 Probesmentioning
confidence: 77%
“…The transposonmediated mobility of rDNA, proposed for Allium (Schubert and Wobus 1985), was supported by permanent clustering of different TEs with 18-5.8-26S or 5S rDNA (Raskina et al 2008). Cestrum strigilatum and C. intermedium exhibited a concentration of these elements in some terminal regions associated with NORs and hybridization signals on both arms of the Bs (Fregonezi et al 2007). This fact confirms the hypotheses that dispersion of 5S and 18-5.8-26S rDNA in Cestrum could have occurred by transposon-mediated transpositional events, being Bs the preferred 'landing sites', due to their neutral character (Jones and Houben 2003).…”
Section: Physical Mapping Of Ribosomal Rna Genes In the Bsmentioning
confidence: 85%
“…Heynh.-type (TTTAGGG) n telomeres, which seem to be replaced by an A/T-rich minisatellite family in Cestrum , Sessea and Vestia , besides an uncommon occurrence of 45S and 5S rDNA in B-chromosomes of Cestrum parqui L'Her. In two other studies, Fregonezi et al (2006 , 2007 ) described different numbers of 45S rDNA sites and the constancy in number and location of 5S rDNA sites in four species of Cestrum , plus the occurrence of a Ty 3- gypsy retrotransposon family dispersed along chromosomes and forming blocks associated with NORs in C. intermedium Sendtn. and C. strigilatum Ruiz & Pav.…”
Section: Introductionmentioning
confidence: 97%
“…Chromosome banding studies have shown that in Cestrum heterochromatin varies in amount and type, and that the similarity observed among karyotypes regarding chromosome size and morphology is apparent (see Berg and Greilhuber, 1992, 1993a, 1993b; Fregonezi et al , 2006). In spite of the small number of species studied , significant information about the occurrence and distribution of different classes of repetitive DNA (45S and 5S rDNA, microsatellites and retroelements) has been reported (Berg and Greilhuber, 1992, 1993a, 1993b; Fregonezi et al , 2006, 2007; Las Peñas et al , 2006). Sykorová et al (2003a, 2003b) described the absence of Arabidopsis (DC.)…”
Section: Introductionmentioning
confidence: 99%