ABC transporters in Mycoplasma hyopneumoniae and Mycoplasma synoviae: insights into evolution and pathogenicity

Nicolás, Marisa Fabiana; Barcellos, Fernando Gomes; Hess, Pablo N.; Hungría, Mariangela

doi:10.1590/s1415-47572007000200006

Cited by 20 publications

(11 citation statements)

References 73 publications

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“…Only 31 (3.65%) of the M. suis CDSs comprise transport systems, which is less than that reported for other mycoplasmas (6.8% to 13.4%) [19], [27], [55]. As expected [55], ABC systems-related sequences represent 74.19% (23/31) of all transporter CDSs. The low number of genes devoted to the transport of nutrients indicates two possibilities: these transport systems may have broad substrate specificities and/or other unknown CDSs are involved in transport of biomolecules through the plasma membrane.…”

Section: Resultsmentioning

confidence: 73%

“…It has been suggested that this reduction has been compensated by using transports with broad substrate specificity [54]. Only 31 (3.65%) of the M. suis CDSs comprise transport systems, which is less than that reported for other mycoplasmas (6.8% to 13.4%) [19], [27], [55]. As expected [55], ABC systems-related sequences represent 74.19% (23/31) of all transporter CDSs.…”

Section: Resultsmentioning

confidence: 96%

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Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an Erythrocyte Niche

et al. 2011

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Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, the objective herein was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways. M. suis was harvested from the blood of an experimentally infected pig. Following DNA extraction and construction of a paired end library, whole-genome sequencing was performed using GS-FLX (454) and Titanium chemistry. Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by PFGE. Glimmer and Manatee Annotation Engine were used to predict and annotate protein-coding sequences (CDS). The M. suis genome consists of a single, 742,431 bp chromosome with low G+C content of 31.1%. A total of 844 CDS, 3 single copies, unlinked rRNA genes and 32 tRNAs were identified. Gene homologies and GC skew graph show that M. suis has a typical Mollicutes oriC. The predicted metabolic pathway is concise, showing evidence of adaptation to blood environment. M. suis is a glycolytic species, obtaining energy through sugars fermentation and ATP-synthase. The pentose-phosphate pathway, metabolism of cofactors and vitamins, pyruvate dehydrogenase and NAD+ kinase are missing. Thus, ribose, NADH, NADPH and coenzyme A are possibly essential for its growth. M. suis can generate purines from hypoxanthine, which is secreted by RBCs, and cytidine nucleotides from uracil. Toxins orthologs were not identified. We suggest that M. suis may cause disease by scavenging and competing for host' nutrients, leading to decreased life-span of RBCs. In summary, genome analysis shows that M. suis is dependent on host cell metabolism and this characteristic is likely to be linked to its pathogenicity. The prediction of essential nutrients will aid the development of in vitro cultivation systems.

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Section: Resultsmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 96%

Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an Erythrocyte Niche

et al. 2011

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“…Therefore, the presence of genes associated with virulence in the core genome helps the organism in adjusting to their ecological niche [ 36 , 37 , 52 , 54 ]. In addition, the oppABCDF transporter and spermidine/putrescine importer genes ( potA , potB and potC ) in the core genome help the organism to transport peptides essential for cell nutrition and viability [ 38 , 39 ], and function in cell proliferation and differentiation, respectively [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Population Genomic Analysis of Mycoplasma bovis Elucidates Geographical Variations and Genes associated with Host-Types

Kumar

Christopher‐Hennings

et al. 2020

Microorganisms

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: Among more than twenty species belonging to the class Mollecutes, Mycoplasma bovis is the most common cause of bovine mycoplasmosis in North America and Europe. Bovine mycoplasmosis causes significant economic loss in the cattle industry. The number of M. bovis positive herds recently has increased in North America and Europe. Since antibiotic treatment is ineffective and no efficient vaccine is available, M. bovis induced mycoplasmosis is primarily controlled by herd management measures such as the restriction of moving infected animals out of the herds and culling of infected or shedders of M. bovis. To better understand the population structure and genomic factors that may contribute to its transmission, we sequenced 147 M. bovis strains isolated from four different countries viz. USA (n = 121), Canada (n = 22), Israel (n = 3) and Lithuania (n = 1). All except two of the isolates (KRB1 and KRB8) were isolated from two host types i.e., bovine (n = 75) and bison (n = 70). We performed a large-scale comparative analysis of M. bovis genomes by integrating 103 publicly available genomes and our dataset (250 total genomes). Whole genome single nucleotide polymorphism (SNP) based phylogeny using M. agalactiae as an outgroup revealed that M. bovis population structure is composed of five different clades. USA isolates showed a high degree of genomic divergence in comparison to the Australian isolates. Based on host of origin, all the isolates in clade IV was of bovine origin, whereas majority of the isolates in clades III and V was of bison origin. Our comparative genome analysis also revealed that M. bovis has an open pangenome with a large breadth of unexplored diversity of genes. The function based analysis of autogenous vaccine candidates (n = 10) included in this study revealed that their functional diversity does not span the genomic diversity observed in all five clades identified in this study. Our study also found that M. bovis genome harbors a large number of IS elements and their number increases significantly (p = 7.8x10-6) as the genome size increases. Collectively, the genome data and the whole genome-based population analysis in this study may help to develop better understanding of M. bovis induced mycoplasmosis in cattle.

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“…The uptake of aminoglycoside antibiotics is a complex process, and the active transport system plays an important role in aminoglycoside antibiotic accumulation (Kashiwagi et al, 1992). As one of the active membrane transport proteins with a conserved ATPase domain, ABC transporters supply energy for the absorption of nutrients and the metabolism of drugs (Nicolás, Barcellos, Hess, & Hungria, 2007). OppA is an oligopeptide ABC transporter substrate-binding protein that constitutes an oligopeptide permease transport system that is responsible for the transport of oligopeptides into cells (Wium, Botes, & Bellstedt, 2015).…”

Section: Discussionmentioning

confidence: 99%

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

et al. 2019

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China launched the Tiangong‐2 space laboratory in 2016 and will eventually build a basic space station by the early 2020s. These spaceflight missions require astronauts to stay on the space station for more than 6 months, and they inevitably carry microbes into the space environment. It is known that the space environment affects microbial behavior, including growth rate, biofilm formation, virulence, drug resistance, and metabolism. However, the mechanisms of these alternations have not been fully elucidated. Therefore, it is beneficial to monitor microorganisms for preventing infections among astronauts in a space environment. Salmonella enteritidis is a Gram‐negative bacterial pathogen that commonly causes acute gastroenteritis in humans. In this study, to better understand the effects of the space environment on S. enteritidis, a S. enteritidis strain was taken into space by the Shenzhou‐11 spacecraft from 17 October 2016 to 18 November 2016, and a ground simulation with similar temperature conditions was simultaneously performed as a control. It was found that the flight strain displayed an increased growth rate, enhanced amikacin resistance, and some metabolism alterations compared with the ground strain. Enrichment analysis of proteome revealed that the increased growth rate might be associated with differentially expressed proteins involved in transmembrane transport and energy production and conversion assembly. A combined transcriptome and proteome analysis showed that the amikacin resistance was due to the downregulation of the oppA gene and oligopeptide transporter protein OppA. In conclusion, this study is the first systematic analysis of the phenotypic, genomic, transcriptomic, and proteomic variations in S. enteritidis during spaceflight and will provide beneficial insights for future studies on space microbiology.

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ABC transporters in Mycoplasma hyopneumoniae and Mycoplasma synoviae: insights into evolution and pathogenicity

Cited by 20 publications

References 73 publications

Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an Erythrocyte Niche

Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an Erythrocyte Niche

Population Genomic Analysis of Mycoplasma bovis Elucidates Geographical Variations and Genes associated with Host-Types

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

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