Genetic characterization of the Spanish Trotter horse breed using microsatellite markers

Azor, P. J.; Valera, M.; Gómez, María Dolores; Goyache, F.; Molina, A.

doi:10.1590/s1415-47572007000100009

Cited by 22 publications

(2 citation statements)

References 35 publications

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“…Although several studies [2, 8, 9, 12] reported population studies on the genetic pedigree structure of several horse breeds, only limited information is available about data needed in casework such as the power of identity and breed assignment [4, 16]. In this study, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker confirmed as the specific alleles unique to Halla horses.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of Halla horses using microsatellite markers

et al. 2016

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BackgroundCurrently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people’s weighted interest toward Jeju horses.MethodUsing 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed.Results and conclusionIn average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of Hobs was 0.749 with a range from 0.612(HMS1) to 0.857(ASB2). Also, it was found that Hexp and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of Hexp was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was 5.90 × 10−20 when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were 4.08 × 10−15 and 3.56 × 10−8, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.Electronic supplementary materialThe online version of this article (doi:10.1186/s40781-016-0120-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity of Halla horses using microsatellite markers

et al. 2016

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“…The microsatellite markers analyzed were 17 loci (Table 1) recommended by the ISAG for equine parentage and identification testing (LEAR et al, 1999;DIMSOSKI, 2003;AZOR et al, 2007;VAN DE GOOR et al, 2010; EQUINE GENETICS AND PARENTAGE ANALYSIS WORKSHOP, 2012). Microsatellites were co amplified with the Equine Genotypes Panel 1.1 (Thermo Scientific), under conditions recommended by the manufacturer.…”

Section: Pcr Amplification and Capillary Electrophoresismentioning

confidence: 99%

Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed

Stanišić¹,

Dimitrijević²,

Simeunović³

et al. 2017

Genetika

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Genetic diversity and population structure of three Indian horse breeds

2010

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The genetic relationships of three Indian horse breeds-Marwari, Spiti, and Kathiawari were studied by genotyping 96 individuals with 20 polymorphic microsatellite markers. A total of 157 alleles were detected across 20 polymorphic loci. The Marwari population showed the highest allelic diversity (A = 5.7 and Ar = 5.14), followed by Spiti (A = 4.9 and Ar = 4.74) and Kathiawari (A = 4.1 and Ar = 3.82). The gene diversity was highest in the Spiti population (He = 0.67), followed by Marwari (He = 0.66) and Kathiawari (He = 0.59). Within population inbreeding estimates (f) in Marwari, Spiti and Kathiawari breeds were 0.18, 0.08, and 0.07, respectively, suggesting high level of inbreeding in these breeds. Analysis of bottleneck revealed evidence of recent bottleneck in Spiti and Kathiawari populations. Pair-wise Fst analysis, AMOVA and assignment tests demonstrated high genetic differentiation and low gene flow between populations. The information about genetic diversity and population structure will be useful for the future development of effective breeding management in order to preserve these Indian horse breeds.

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Genetic characterization of the Spanish Trotter horse breed using microsatellite markers

Cited by 22 publications

References 35 publications

Genetic diversity of Halla horses using microsatellite markers

Genetic diversity of Halla horses using microsatellite markers

Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed

Genetic diversity and population structure of three Indian horse breeds

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