2006
DOI: 10.1590/s1415-47572006000400023
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Abstract: The Gram-negative bacterium Pseudomonas aeruginosa has a wide environmental and ecological distribution. It is an opportunistic pathogen that acquires resistance to multiple antimicrobial agents and can infect plants, animals and humans. We used rDNA and tDNA PCR markers to characterize the bacterial diversity of P. aeruginosa strains isolated at a Brazilian teaching hospital (Oswaldo Cruz University Hospital, Recife, Brazil) between March 2003 and February 2004. Clonal groups of P. aeruginosa clinical isolate… Show more

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Cited by 4 publications
(10 citation statements)
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References 35 publications
(29 reference statements)
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“…In addition to the research of resistance genes, another indispensable tool in controlling infections is the application of genotyping methods for the determination of genetic relationships among the microorganisms, thus allowing mapping the dynamics of infection transmission 16,17 . The utilization of the enterobacterial repetitive intergenic consensus sequence by polymerase chain reaction (ERIC-PCR) has demonstrated its efficacy, efficiency, and usefulness for epidemiological surveillance [17][18][19] .…”
Section: Introductionmentioning
confidence: 99%
“…In addition to the research of resistance genes, another indispensable tool in controlling infections is the application of genotyping methods for the determination of genetic relationships among the microorganisms, thus allowing mapping the dynamics of infection transmission 16,17 . The utilization of the enterobacterial repetitive intergenic consensus sequence by polymerase chain reaction (ERIC-PCR) has demonstrated its efficacy, efficiency, and usefulness for epidemiological surveillance [17][18][19] .…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, some authors have emphasized the importance of tDNA-PCR as a tool for the identification of bacterial species. On the other hand, different profiles were detected by tDNA-PCR for S. aureus, Staphylococcus haemolyticus (Maes et al 1997), Bacillus licheniformis (Daffonchio et al 1998) and P. aeruginosa (Spacov et al 2006). In the present report tDNA-PCR allowed the discrimination of the clinical K. pneumoniae isolates from Brazil into three major patterns, with a predominance of the group that showed the same profile as the K. pneumoniae reference strains.…”
Section: Discussionmentioning
confidence: 45%
“…Meanwhile, this is expensive and requires a high degree of technical competency to perform (Olive & Bean 1999) and therefore not recommended for routine identification of bacteria. In contrast, PCR-based methods have been widely used for the analysis of the genetic diversity of many microorganisms (Agodi et al 2000, Souza Lopes et al 2005, Spacov et al 2006. Depending on the primers and amplification conditions employed, the results allow discrimination of organisms at the genus, species or strain level.…”
mentioning
confidence: 99%
“…Besides, they are grouped and disconnected by spacers whose length and sequences are subjected to great variation. 10 Assessment of the efficiency of the typing method depends on several factors such as typeability and discrimination. Typeability is simply the percentage of bacterial isolates that can be positively typed by the typing marker.…”
Section: Introductionmentioning
confidence: 99%