Characterization of different strains of common carp (Cyprinus carpio L.) (Cyprinidae, Cypriniformes) in Bangladesh using microsatellite DNA markers

Abstract

Characterization of different strains of common carp (Cyprinus carpio L.) using molecular markers is essential for the management of this fish in respect to the evaluation of the potential genetic effects induced by hatchery operations and the genetic improvement of carp varieties. Five microsatellite loci (MFW1, MFW2, MFW11, MFW15 and MFW20) were analyzed for the molecular characterization of four common carp strains, i.e. scaled carp, mirror carp, red carp and koi carp. We observed differences in heterozygosities and the average numbers of alleles but not in polymorphic loci (P 95 ) among the strains. Koi carp displayed the highest level of variability in terms of heterozygosity. The N m values and the F ST values indicated a low level of gene flow and high level of differentiation among the strains. The highest genetic distance was observed between the scaled carp and the koi carp whilst the lowest genetic distance was found between the red-and koi carp. The unweighted pair group method with averages (UPGMA) dendrogram resulted in two clusters, one containing only the scaled carp and the other the remaining three varieties. Microsatellite markers have been found to be effective tools for characterization of different strains of common carp.

Genetic Variability Comparison of Wild and Cultured Far Eastern Catfish (Silurus asotus) of Korea using Microsatellite Marker

“…In contrast, the cultured group continued its own production without the introduction of new wild broodstock, and therefore they are considered to be genetically distant from the wild group. Similar results have been reported with cultured carp ( Ma et al, 2006 ; Mondol, 2006 ).…”

“…The wild group in Korea was not as diverse as the Chinese wild samples, but the diversity was comparable to the general wild common carp family ( N A , 16–26) ( Biba et al, 2015 ; Ghelichpour et al, 2013 ). According to a previous study, mortality was reported to occur frequently in groups with N A values ranging from 5 to 9 in the cultured group ( Mondol et al, 2006 ). The diversity of domestic farm groups in our study did not show a value that would exhibit fatality of the species, but it continued to decrease; hence, caution should be exercised to maintain this diversity.…”

The common carp, Cyprinus carpio, in the Mediterranean region: origin, distribution, economic benefits, impacts and management

“…As the oldest domesticated fish (Balon 2004, 2006), carp has been subjected to many genetic interventions (cf. Chistiakov & Voronova 2009), along with geographical isolation, adaptation, accumulation of mutations and natural as well as human selection pressures (Mondol et al. 2006).…”

Karakteristik Fenotipe Dan Genotipe Lima Strain Ikan Mas Di Jawa Barat Dan Banten

“…Private allele merupakan alel yang hanya ditemukan pada satu strain dan tidak ditemukan pada strain lainnya (Syahputra et al, 2016) Jumlah rata-rata alel setiap strain ikan mas dalam penelitian ini berbeda-beda, berkisar antara 5,8 pada strain Majalaya hingga 8,8 pada strain Wildan (Tabel 2). Rata-rata jumlah alel ini relatif sama jika dibandingkan dengan rata-rata jumlah alel yang teridentifikasi pada populasi ikan mas di Asia dan Eropa (Kohlmann et al, 2005), Bangladesh (Mondol et al, 2006), dan Vietnam (Thai et al, 2007). Frekuensi rata-rata alel pada strain Rajadanu sama dengan strain Sinyonya, mengindikasikan bahwa kedua strain tersebut mempunyai struktur genotipe yang relatif sama, tetapi berbeda dengan tiga strain lainnya.…”