Phylogenetic relationships and karyotype evolution in the sigmodontine rodent Akodon (2n = 10 and 2n = 16) from Brazil

Silva, Maria José de J.; Patton, James L.; Yonenaga‐Yassuda, Yatiyo

doi:10.1590/s1415-47572006000300012

Cited by 18 publications

(20 citation statements)

References 25 publications

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“…The phenotypic uniformity stays in contrast to the high karyotypic divergence observed in these rodents, ranging from 2n = 10 to 2n = 46 (Sbalqueiro et al 1986, Christoff et al 2000. The number of autosomal chromosome arms (FNa = 14Y16 to 46) (Sbalqueiro & Nascimento 1996, Silva et al 2006, makes this karyotype divergence even more intricate.…”

Section: Introductionmentioning

confidence: 93%

Chromosomal phylogeny of four Akodontini species (Rodentia, Cricetidae) from Southern Brazil established by Zoo-FISH using Mus musculus (Muridae) painting probes

2008

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We established chromosome homology maps between Mus musculus (MMU) and five species of the Akodontini tribe, Akodon cursor (2n = 14, 15 and 16), A. montensis (2n = 24), A. paranaensis (2n = 44), A. serrensis (2n = 46) and Oligoryzomys flavescens (2n = 66) by Zoo-FISH (fluorescence in situ hybridization) using mouse chromosome-specific probes. The aims of this study were (1) to detect the chromosomal rearrangements responsible for the karyotype variation in this tribe and (2) to reconstruct the phylogenetic relationships among these species. We observed four common syntenic associations of homologous chromosome segments, of which the MMU 7/19 has been described previously in other rodents from Africa, Asia and Europe, and might represent a phylogenetic link between the Old World and Neotropical rodents. The remaining three associations (3/18, 6/12 and 8/13) have been observed exclusively in Neotropical rodents so far, which at present can be considered synapomorphic traits of this group. Five further mouse chromosomes (MMU 4, 9, 14, 18 and 19) were each found evolutionarily conserved as a separate syntenic unit. Our phylogenetic analysis using parsimony and heuristic search detected one consistent group, separating the Akodontini from other rodents.

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Section: Introductionmentioning

confidence: 93%

Chromosomal phylogeny of four Akodontini species (Rodentia, Cricetidae) from Southern Brazil established by Zoo-FISH using Mus musculus (Muridae) painting probes

2008

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“…A comparison between the karyotype of Akodon sp. n. and A. cursor was made by Silva et al (2006), but the authors were unable to establish complete correspondence between both complements since a distal part of pair 3 of the former species did not show homology to any chromosome pair of the latter using G banding. After telomeric FISH, both homologs of pairs ASP 1 and ASP 3 showed ITS, which did not coincide with the presumed breakpoints involved in the proposed chromosomal rearrangements.…”

Section: Introductionmentioning

confidence: 99%

Chromosome homologies of the highly rearranged karyotypes of four Akodon species (Rodentia, Cricetidae) resolved by reciprocal chromosome painting: the evolution of the lowest diploid number in rodents

et al. 2009

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Traditionally comparative cytogenetic studies are based mainly on banding patterns. Nevertheless, when dealing with species with highly rearranged genomes, as in Akodon species, or with other highly divergent species, cytogenetic comparisons of banding patterns prove inadequate. Hence, comparative chromosome painting has become the method of choice for genome comparisons at the cytogenetic level since it allows complete chromosome probes of a species to be hybridized in situ onto chromosomes of other species, detecting homologous genomic regions between them. In the present study, we have explored the highly rearranged complements of the Akodon species using reciprocal chromosome painting through species-specific chromosome probes obtained by chromosome sorting. The results revealed complete homology among the complements of Akodon sp. n. (ASP), 2n = 10; Akodon cursor (ACU), 2n = 15; Akodon montensis (AMO), 2n = 24; and Akodon paranaensis (APA), 2n = 44, and extensive chromosome rearrangements have been detected within the species with high precision. Robertsonian and tandem rearrangements, pericentric inversions and/or centromere repositioning, paracentric inversion, translocations, insertions, and breakpoints, where chromosomal rearrangements, seen to be favorable, were observed. Chromosome painting using the APA set of 21 autosomes plus X and Y revealed eight syntenic segments that are shared with A. montensis, A. cursor, and ASP, and one syntenic segment shared by A. montensis and A. cursor plus five exclusive chromosome associations for A. cursor and six for ASP chromosome X, except for the heterochromatin region of ASP X, and even chromosome Y shared complete homology among the species. These data indicate that all those closely related species have experienced a recent extensive process of autosomal rearrangement in which, except for ASP, there is still complete conservation of sex chromosomes homologies.

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“…The karyotype of a new species of Akodon with 2n = 10 was compared to the A. cursor 2n = 16 karyotype and rearrangements such as pericentric inversions, tandem fusions, and loss of telomeres were proposed, supported by the analysis of telomeric FISH, Ag-NORs and G-banding (Silva and Yonenaga-Yassuda, 1998a;Silva et al, 2006). ITS were observed in the 2n = 10 karyotype in addition to the telomeric signals, however those ITS do not seem to correspond to the exact putative positions involved in the chromosomal rearrangements.…”

mentioning

confidence: 99%

Non-telomeric sites as evidence of chromosomal rearrangement and repetitive (TTAGGG)<sub>n </sub>arrays in heterochromatic and euchromatic regions in four species of <i>Akodon</i> (Rodentia, Muridae)

Ventura¹,

Silva²,

Fagundes³

et al. 2006

Cytogenet Genome Res

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Comparative studies among four species – Akodonazarae (2n = 38), A. lindberghi (2n = 42), A. paranaensis (2n = 44) and A. serrensis (2n = 46) – employing classic cytogenetics (C- and G-bands) and fluorescence in situ hybridization with telomeric (TTAGGG)_n sequencesare reported here. Non-telomeric signals in addition to the regular telomeric sites were detected in three species:A. azarae, A. lindberghi and A. serrensis. One interstitial telomeric site (ITS) was observed proximally at the long arm of chromosome 1 of A. azarae. The comparison of G-banding patterns among the species indicated that the ITS was due to a tandem fusion/fission rearrangement. Non-telomeric signals of A. lindberghi and A. serrensis were not related to chromosomal rearrangements; instead, the sequences co-localized with (i) heterochromatic regions of all chromosomes in A. serrensis; (ii) some heterochromatic regions in A. lindberghi, and (iii) both euchromatic and heterochromatic regions in the metacentric pair of A. lindberghi. These exceptional findings revealed that ITS in Akodon can be related to chromosomal rearrangements and repetitive sequences in the constitutive heterochromatin and that the richness of TTAGGG-like sequences in the euchromatin could be hypothesized to be a result of amplification of the referred sequence along the chromosome arms.

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Phylogenetic relationships and karyotype evolution in the sigmodontine rodent Akodon (2n = 10 and 2n = 16) from Brazil

Cited by 18 publications

References 25 publications

Chromosomal phylogeny of four Akodontini species (Rodentia, Cricetidae) from Southern Brazil established by Zoo-FISH using Mus musculus (Muridae) painting probes

Chromosomal phylogeny of four Akodontini species (Rodentia, Cricetidae) from Southern Brazil established by Zoo-FISH using Mus musculus (Muridae) painting probes

Chromosome homologies of the highly rearranged karyotypes of four Akodon species (Rodentia, Cricetidae) resolved by reciprocal chromosome painting: the evolution of the lowest diploid number in rodents

Non-telomeric sites as evidence of chromosomal rearrangement and repetitive (TTAGGG)<sub>n </sub>arrays in heterochromatic and euchromatic regions in four species of <i>Akodon</i> (Rodentia, Muridae)

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