2005
DOI: 10.1590/s1415-47572005000500014
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Abstract: Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (½ HF x ½ Hariana). In all the cattle we amp… Show more

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Cited by 29 publications
(36 citation statements)
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“…of the present study in enzyme digestion and observed fragment length agreed with those in Norwegian cattle (17) and in Indian Bos indicus and Bos taurus cattle (20) . Figure 1 shows separation of 522, 441 and 81 bp fragments and restriction pattern of three genotypes AA, AB and BB upon digestion of the leptin locus using BsaAI restriction enzyme.…”
Section: Resultssupporting
confidence: 89%
See 1 more Smart Citation
“…of the present study in enzyme digestion and observed fragment length agreed with those in Norwegian cattle (17) and in Indian Bos indicus and Bos taurus cattle (20) . Figure 1 shows separation of 522, 441 and 81 bp fragments and restriction pattern of three genotypes AA, AB and BB upon digestion of the leptin locus using BsaAI restriction enzyme.…”
Section: Resultssupporting
confidence: 89%
“…The results de digestión, incluyendo el patrón A, un fragmento intacto de 522 bp (sin sitio de corte de la enzima) y un patrón B que exhibió fragmentos de 441 y 81 bp. Los resultados del presente estudio en la digestión de la enzima y longitud del fragmento observado concuerdan con los del ganado Noruego (17) y el Indio Bos indicus y Bos taurus (20) . La Figura 1 muestra la separación de fragmentos 522, 441 y 81 bp y el patrón de restricción de tres genotipos AA, AB y BB sobre la digestión del locus leptina usando la enzima de restricción BsaAI.…”
Section: Resultsunclassified
“…A recent study in Brazilian Nellore cattle of E2FB marker reported frequencies of 0.88 for allele C and 0.12 for allele T (Souza et al, 2010), which are close to data reported in this study. E2FB marker was polymorphic in Brazilian Nellore cattle diverging from the hypothesis of Choudhary et al (2005); this is probably due to the participation of Bos taurus dams in the formation of this breed in Brazil, as reported by Meirelles et al (1999). This is the first study describing and studying the association of carcass traits with polymorphisms in the promoter region of the leptin gene in Nellore cattle.…”
Section: Descriptive Analysis and Allelic And Genotypic Frequenciesmentioning
confidence: 71%
“…Choudhary et al (2005) studied the allelic and genotypic frequencies of E2FB marker and reported the fixation of the C allele in Hariana, Sahiwal, Gyr, Table 1. Descriptive analysis for growth and carcass traits.…”
Section: Descriptive Analysis and Allelic And Genotypic Frequenciesmentioning
confidence: 99%
“…The y-intercept (a) estimates the average value of the CC genotype and dominance deviations (d) are estimated by the difference between observed and predicted values at the TC genotype. (Buchanan et al, 2003); Angus, 0.58;Herford, 0.55;Charolais, 0.34;and Simmental, 0.32 (Buchanan et al, 2002) and Holstein Friesien cattle, 0.40; Jersey, 0.56 (Choudhary et al, 2005) and Polish Black and White proven bulls 0.46 (Madeja et al, 2004).…”
Section: Methodsmentioning
confidence: 99%