Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

Silva-Barbosa, Isvânia; Pereira-Magnata, Símey; Amaral, Ademir; Sotero, Graça; Melo, Homero Cavalcanti

doi:10.1590/s1415-47572005000300021

Cited by 21 publications

(15 citation statements)

References 13 publications

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“…cervical cancer, prostate cancer or Hodgkin's disease. The doses estimated by MN analysis agree quite well with averaged whole body doses calculated from the radiation treatment plans (8,(42)(43)(44)(45). Studies performed in thyroid cancer patients undergoing radioiodine treatment further demonstrated that the CBMN assay is sensitive enough to detect low average whole-body doses from internal exposure scenarios (16,23,46).…”

Section: Applications Of the Cbmn Assay For Biological Dosimetrysupporting

confidence: 57%

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

Vral

Fenech

Thierens³

2010

Mutagenesis

216

133

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Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus (MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected. In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.

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Section: Applications Of the Cbmn Assay For Biological Dosimetrysupporting

confidence: 57%

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

Vral

Fenech

Thierens³

2010

Mutagenesis

216

133

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“…The number of MN in PBMCs of endometrial patients increased significantly after each RT fraction that we examined. Similarly, Silva-Barbosa et al assessed five uterine cancers patients irradiated by the absorbed dose of 0 Gy, 0.08 Gy, and 1.8 Gy, respectively, and found statistical increase in DC and MN, respectively [ 29 ]. A significant work has been conducted in the area of MN analysis and high-throughput biodosimetry by Dr. Brenner’s group [ 30 , 31 ], who recently reported an implementation of commercial robotic high-throughput screening system for large-scale radiological incidents [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

The first in vivo multiparametric comparison of different radiation exposure biomarkers in human blood

et al. 2018

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The increasing risk of acute large-scale radiological/nuclear exposures of population underlines the necessity of developing new, rapid and high throughput biodosimetric tools for estimation of received dose and initial triage. We aimed to compare the induction and persistence of different radiation exposure biomarkers in human peripheral blood in vivo. Blood samples of patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before the first treatment and then after 24 h, 48 h, and 5 weeks; i.e. after 1, 2, and 25 fractionated RT procedures. We collected circulating peripheral blood from ten patients with tumor of endometrium (1.8 Gy per fraction) and eight patients with tumor of head and neck (2.0–2.121 Gy per fraction). Incidence of dicentrics and micronuclei was monitored as well as determination of apoptosis and the transcription level of selected radiation-responsive genes. Since mitochondrial DNA (mtDNA) has been reported to be a potential indicator of radiation damage in vitro, we also assessed mtDNA content and deletions by novel multiplex quantitative PCR. Cytogenetic data confirmed linear dose-dependent increase in dicentrics (p < 0.01) and micronuclei (p < 0.001) in peripheral blood mononuclear cells after PBI. Significant up-regulations of five previously identified transcriptional biomarkers of radiation exposure (PHPT1, CCNG1, CDKN1A, GADD45, and SESN1) were also found (p < 0.01). No statistical change in mtDNA deletion levels was detected; however, our data indicate that the total mtDNA content decreased with increasing number of RT fractions. Interestingly, the number of micronuclei appears to correlate with late radiation toxicity (r2 = 0.9025) in endometrial patients suggesting the possibility of predicting the severity of RT-related toxicity by monitoring this parameter. Overall, these data represent, to our best knowledge, the first study providing a multiparametric comparison of radiation biomarkers in human blood in vivo, which have potential for improving biological dosimetry.

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“…Por exemplo, trabalhadores expostos ocupacionalmente a substâncias como benzeno e outros solventes orgânicos têm uma maior incidência de aberrações cromossômicas em comparação à população em geral 8 . Essas alterações citogenéticas são também utilizadas como indicadores de efeitos precoces nos estudos de monitoramento biológico em populações expostas a diversos agentes genotóxicos 7,10 , como verificado em (poucos) estudos brasileiros [55][56][57][58][59][60][61][62][63][64][65] .…”

Section: ▄ Discussãounclassified

Alterações respiratórias, auditivas e citogenéticas em trabalhadores de um estaleiro no Rio de Janeiro: estudo de caso

et al. 2017

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Resumo Introdução Este estudo avaliou alterações respiratórias, auditivas e citogenéticas em trabalhadores de um estaleiro em Angra dos Reis, no Estado do Rio de Janeiro, relacionadas à exposição de pintores a solventes, metais e ruído no ambiente de trabalho. Métodos Foram avaliados função pulmonar, perdas auditivas e índice de reconhecimento da fala, e alterações citogenéticas pelo teste de aberrações cromossômicas. Foram avaliados manganês e chumbo em sangue por espectrometria de absorção atômica. Os indicadores de efeito utilizados para chumbo foram ALAD e ALA-U, determinados por espectrofotometria e cromatografia líquida, respectivamente. Resultados Seis dos 9 trabalhadores avaliados apresentaram alteração funcional respiratória. Quase 70% dos 18 trabalhadores avaliados apresentaram audição reduzida, com associação entre PAIR e chumbo em sangue. O percentual médio de recuperação da ALAD foi de 32,9%, com médias de ALA-U de 1,7 mg g-1 creatinina, 4,65 µg dL-1 para Pb-S e 10 µg L -1 para Mn-S entre os trabalhadores. Foram observadas associações entre ALA-D ativada com Mn-S e com a presença de aberrações cromossômicas. As alterações citogenéticas identificadas foram aneuploidias, separação prematura centromérica e aberrações como formação de anéis, quebras e união de cromátides irmãs. Conclusão os trabalhadores do estaleiro apresentam alterações que podem ser associadas à exposição ocupacional.

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Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

Cited by 21 publications

References 13 publications

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

The first in vivo multiparametric comparison of different radiation exposure biomarkers in human blood

Alterações respiratórias, auditivas e citogenéticas em trabalhadores de um estaleiro no Rio de Janeiro: estudo de caso

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