Use of the TSPY gene for sexing cattle

Lemos, Daniela Cristina; Rios, Álvaro Fabrício Lopes; Caetano, Leony Cristina; Lôbo, Raysildo Barbosa; Vila, Reginaldo Aparecido; Martelli, Lúcia Regina; Takeuchi, Paula Lumy; Ramos, Ester Silveira

doi:10.1590/s1415-47572005000100020

Cited by 16 publications

(16 citation statements)

References 17 publications

(23 reference statements)

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“…In a previous study, we reported that PCR using primers derived from a TSPY sequence allows the detection of the Y chromosome in male animals (Nelore cattle) even at low DNA concentrations. These preliminary results showed the potential of this gene as a male-specific marker (Lemos et al, 2005).…”

Section: Discussionmentioning

confidence: 58%

“…The application of polymerase chain reaction (PCR) for the analysis of a single cell presents a challenge since limited DNA is available in one single diploid cell, and this can lead to a reduction in amplification efficiency. Lemos et al (2005) described a study on the TSPY (testis-specific protein Y-encoded) gene as a male-specific marker for cattle sexing in very low DNA concentrations. The TSPY gene is advantageous compared to other markers because it is a Y-specific gene of multiple copies both in human and bovine genomes.…”

Section: Introductionmentioning

confidence: 99%

“…The TSPY gene is advantageous compared to other markers because it is a Y-specific gene of multiple copies both in human and bovine genomes. After embryo biopsy, the DNA from only one cell is amplified, so, using genes with multiple copies, we could increase the efficiency of the amplification (Bartmann et al, 2004;Lemos et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we tested a nested PCR-based protocol, modified from Lemos et al (2005), in order to sex single cells from bovine preimplantation embryos using a sequence of the TSPY gene (also known as DYS14 in humans).…”

Section: Introductionmentioning

confidence: 99%

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Sexing single bovine blastomeres using TSPY gene amplification

Carneiro

Takeuchi²,

Araújo³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same embryo were concordant in all cases. All blastomeres (16/16) from eight embryos produced with sexed sperm (specific for production of male embryos) were TSPY-positive. We conclude that TSPY is a good male-specific marker, the usefulness of which is probably enhanced by the high copy number. Other methods that are less time-consuming, such as real-time PCR, could be improved with the use of the TSPY gene sequences to generate primers and/or probes. This is the first report to demonstrate the applicability of the TSPY gene for sexing single cells in cattle.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sexing single bovine blastomeres using TSPY gene amplification

Carneiro

Takeuchi²,

Araújo³

et al. 2011

Genet. Mol. Res.

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“…Por otro lado, la detección del sexo a partir de una muestra de ADN mediante PCR puede ser realizada usando primers específicos de distintos genes como el SRY, TSPY1, y otros. Dado que el gen TSPY1 es multicopia, es decir, presenta varias copias en bajas concentraciones de ADN, resulta más útil y eficiente como marcador masculino a diferencia de otros genes como el SRY que es de 1 sola copia (Lemos et al, 2005).…”

Section: Montano Vizcarra D Y Colunclassified

Amplificación del gen TSPY1 como método de sexaje en bovinos

Vizcarra¹,

Chaparro²

2018

Salud tecnol. vet.

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RESUMENLa investigación tuvo como objetivo optimizar un protocolo existente para el sexaje de ganado bovino usando primers específicos para el gen TSPY1 mediante la técnica de Reacción en Cadena de la Polimerasa (PCR). Para este propósito, muestras de sangre fueron obtenidas de centros de beneficio en la ciudad de Lima. El tamaño de la muestra fue de 60 animales (30 machos y 30 hembras). Así mismo, la técnica fue probada en muestras de ADN de embriones. El ADN extraído de muestras provenientes de animales machos mostró una amplificación del gen TSPY1 mientras que aquel obtenido de animales hembras no mostró dicha amplificación. Similares resultados fueron obtenidos de muestras de ADN obtenidas de embriones.PALABRAS CLAVE: Gen TSPY1, sexaje, PCR, transferencia embrionaria, bovino. SUMMARYThis research was undertaken to optimize a previously described protocol for sexing cattle using the Polymerase Chain Reaction (PCR) molecular technique with specific TSPY1 gene primers. For this purpose, blood samples were obtained from slaughterhouses in the city of Lima. The sample size was 60 animals (30 males and 30 females). Likewise, the technique was tested in DNA samples obtained from embryos. The extracted DNA obtained from male samples showed an amplification of the TSPY1 gen while the one obtained from female samples did not show any amplification. Similar results were obtained from DNA samples obtained from embryos.KEY WORDS: TSPY1 gen, sexing, PCR, embryo transfer, bovine. INTRODUCCIÓNLos sistemas de producción bovina intensivos buscan obtener altos rendimientos, ya sea en ganado de carne como de leche, en el menor tiempo posible y minimizando costos de producción. En ese aspecto, los temas reproductivos juegan un rol importante para asegurar la progenie según el tipo de producción. Para ello se cuenta con la inseminación artificial usando semen de toros previamente seleccionados de acuerdo a las características productivas deseadas y la transferencia embrionaria que viene siendo utilizada en distintos países de Latinoamérica, la cual involucra el correcto sexaje de los embriones antes de ser transferidos (Almeyda y Díaz, 2006).El sexaje de las crías brinda muchas oportunidades para el productor porque permite seleccionar a los animales más adecuados para los sistemas de producción que se requieran (Divar, Sharifiyazdi y Kafi, 2012). Una manera práctica y eficiente de realizarlo es mediante la identificación del cromosoma Y que permite reconocer a los machos y diferenciarlos de las muestras que no amplifican dicho cromosoma, las cuales son clasificadas como hembras (Hamilton et al., 2011).

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