Somatic and germ cell cytogenetic studies and AZF microdeletion screening in infertile men

Abstract

Clinical and cytogenetic studies were performed in 65 infertile individuals, and 56 of them were also screened for microdeletions in Yq11 (AZF region). Relevant environmental etiological factors were identified in 10 cases (15.4%). Sertoli-cell-only syndrome was diagnosed in six patients (9,2%). Karyotype abnormalities were detected in six individuals, and five other patients presented desynapsis of bivalents in meiosis. Three out of the 56 patients studied were carriers of microdeletions in the AZF region, one of them also presenting a chromosomal mosaicism for an extra i(22p).

Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males

“…Vogt considered the oligozoospermic criteria less than 2 mil/ml sperm in his study [7]. In Other investigations oligozoospermic criteria was considered less than one mil/ml, which obviously can highly influence the results of the studies [23,24]. In our study less than 20 mil/ml was considered as oligozoospermia, which may cause significant increase in the amount of gonadotropin hormones and decreased testosterone serum level in compare to normal fertile group.…”

Analysis of Partial AZFc (gr/gr, b1/b3, and b2/b3) Deletions in Iranian Oligozoospermia Male Patients as The Candidates of Intra-Cytoplasmic Sperm Injection (ICSI)

“…However, there are several factors that affect these results, such as demographic variations, differences in patient selection values, AZF in variable deletion regions, applying different STS markers, and environmental effects, and also the classification method used to define oligospermia with different amount of sperms varied, i.e. 5 × 10 6 , 2 × 10 6 , and 1 × 10 6 , where Vog considered 2 × 10 6 > sperms per milliliter as an oligospermia standard (23) and in other studies performed by Carrara (24) and Hellani (25) this standard was considered 1 × 10 6 > sperms per milliliter, which certainly had an important influence on the results of these studies (26). However, in the present study, the standard for selecting patients among the oligospermia men was a sperm count of less than 20 × 10 6 per milliliter.…”

Y-chromosome microdeletion and male infertility: a systematic review

“…Studies have good methodological accuracy. The sample universe is quite heterogeneous, given the very design of the studies (22 case reports with 02 individuals up to 33 cross-sectional studies with 4, 441 individuals), age differences between individuals in the samples ranging from 17 years [10] to 66 years [21], as well as the origin of the patients who were: India [2,23,25,35], Brazil [3,31,34,36], China [4,10,17,18], Iran [1,7,19,21] Turkey [5,26,27] [20], Denmark [37], USA [22], england [38], Mexico [30], Serbia [28] and Venezuela [32]. Because of this situation, the data becomes divergent and the comparative analysis becomes troublesome.…”

“…al. [34] highlight the following chromosomal mosaicism for patient # I-9, 47, XY, +i(22p) [10]/46, XY [90]. In this sample [34], three patients had Y-chromosome microdeletion.…”

“…[34] highlight the following chromosomal mosaicism for patient # I-9, 47, XY, +i(22p) [10]/46, XY [90]. In this sample [34], three patients had Y-chromosome microdeletion. Mozdarani and Ghoraeian [7] simultaneously detected the DAZ gene (Deleted in azoospermia) in centromeres of X and Y chromosomes, and aneuploidies.…”