Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays

Teixeira, Rosangela de Oliveira; Camparoto, Marjori Leiva; Mantovani, Mário; Vicentini, Verônica Elisa Pimenta

doi:10.1590/s1415-47572003000400021

Cited by 129 publications

(96 citation statements)

References 10 publications

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“…Allium cepa L. has been used as a first-tier assay for the detection of environmental chemicals that may pose genetic hazards from a wide range of pollutants such as sewage effluents (Ukaegbu and Odeigah, 2009), leacheates Wale-Adeyemo, 2004, Chandra et al, 2005) and chemicals (Seetharaman et al, 2004). Cytotoxicity and environmental pollution have been assessed by the in vivo onion (Allium cepa) root tip cell test which is known to give similar results to in vitro animal cytotoxicity tests (Vicentini et al, 2001;Teixeira et al, 2003). A. cepa test has also been used by many authors to evaluate cytotoxicity and genotoxicity of industrial effluents (Rank and Nielson, 1998;Chauhan et al, 1999;Grover and Kaur, 1999;El-Shahaby et al, 2003, Babatunde andBakare, 2006;Junior et al, 2007;Abdel-Migid et al, 2007;Şık et al, 2009;Samuel et al, 2010).…”

Section: Industriesmentioning

confidence: 99%

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Olorunfemi¹,

Ogieseri²,

Akinboro³

2011

Journal of Applied Sciences and Environmental Management

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ABSTRACT:The potential cytotoxicity and genotoxicity of three industrial wastewaters (brewery, rubber and bottling) in Benin metropolis using the Allium cepa test were investigated. A series of five small onion bulbs were cultivated in 0.01, 0.1, 1, and 10% of the wastewaters (v/v) and after 48 hours, one root tip from each bulb was harvested and processed for cytological studies by the aceto-orcein squash technique. At 72h, their cytotoxic effects on the root tips showed strong growth retardation in high concentrations of all the wastewaters. Compared to the control, treatment with the wastewaters resulted in root growth inhibition with EC50 values of 35, 50 and 62% for bottling, rubber and brewery effluents respectively, and decrease in mitotic index with increasing concentration for all samples and these were statistically significant (p<0.05). Chromosomal aberrations induced in the onion root tip cells were mostly sticky chromosomes and bridges. Chromosomes with disturbed spindles and fragments were also present in appreciable amounts. Based on the EC50 values, the bottling wastewater was most toxic, followed by rubber effluent while effluents from the brewery were least toxic. The findings in this study indicate that there are toxic chemicals present in the wastewaters which are responsible for the observed genotoxic effects on the onion root tip cells. The study also reveals that the Allium test is a useful and reliable tool for the genotoxicity screening of industrial effluents which could be employed by environmental managers before these effluents are finally discharged into the environment. @JASEM

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Section: Industriesmentioning

confidence: 99%

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Olorunfemi¹,

Ogieseri²,

Akinboro³

2011

Journal of Applied Sciences and Environmental Management

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“…Sistemas testes vegetais, principalmente o de A. cepa, têm sido utilizados para estudos preliminares dos efeitos de extratos vegetais, visando à detecção do potencial de genotoxicidade (Teixeira et al, 2003;Fachinetto et al, 2007;Frescura, 2012), servindo como primeira triagem de detecção. O bioensaio é considerado uma das abordagens mais eficientes e é rotineiramente usado para determinar os efeitos tóxicos de compostos químicos no ambiente (Leme & Marin-Morales, 2009).…”

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Efeito genotóxico e antiproliferativo de Mikania cordifolia (L. F.) Willd. (Asteraceae) sobre o ciclo celular de Allium cepa L.

Dias

Canto-Dorow

Coelho

et al. 2014

Rev. bras. plantas med.

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INTRODUÇÃOAs plantas com potencial medicinal têm sido muito utilizadas para o tratamento de doenças no Brasil, sendo, algumas vezes, o único medicamento disponível à população (Fachinetto & Tedesco, Efeito genotóxico e antiproliferativo de Mikania cordifolia (L. For each one, two treatments were prepared in different concentrations: 4g/500mL and 16g/500mL. A positive control consisting of 10% glyphosate in 90% water, which is an herbicide widely used and known for its genotoxic potential, was also prepared. Distilled water was used as negative control. After a 24-hour period, the infusion of the root tips was collected, fixed in ethanol-acetic acid (3:1) for 24 hours and stored in 70% ethanol. Cells were analyzed in all phases of the cell cycle of A. cepa, a total of 2500, for each group of bulbs. The mitotic index (MI) was calculated and statistically analyzed by the c 2 test at 5%. Results showed that, in both populations of M. cordifolia, a reduction of the MI in all treatments compared with the negative control was observed. In both populations, an increase in the mitotic index values was obtained with increasing concentration of the infusion. Chromosomal aberrations were observed in both populations studied. In conclusion, infusions of M. cordifolia possess antiproliferative and mutagenic effects for the concentrations tested on the cell cycle of A. cepa.

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“…This, ayurvedic product may have better antioxidant activity endow with the ability to intercept the free radicals generated by cellular metabolism or exogenous sources, such as those resulting from the action of cyclophosphamide, thereby preventing their damage to lipids, amino acids, proteins, polyunsaturated fatty acid double bonds, and DNA bases [25]. The aqueous extracts of PM and BF can act directly on compounds that induce mutations in DNA, chemically or enzymatically inactivating them, may inhibit the metabolic activation of promutagenic agents, or may scavenge reactive molecules, as explained [23].…”

Section: Discussionmentioning

confidence: 99%

Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

Manipura

2017

Asian J Pharm Clin Res

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Objective: Liv52, a nontoxic herbal preparation is reported to be clinically active in hepatotoxicity and a wide range of hepatic disorders. This study was undertaken to evaluate the mutagenic/antimutagenic potential of Liv52 using Salmonella mutagenicity test. Methods:Ames Salmonella/Mammalian -microsome mutagenicity test was used to evaluate the mutagenic or antimutagenic potential of Liv52. Salmonella typhimurium tester strains TA1537, TA1538, TA100, and TA102 were used for mutagenicity testing. The antimutagenicity study was carried out in tester strains TA1538 and TA100 against various standard mutagens with and without metabolic activation.Results: Liv52 did not show any mutagenic potential both with and without metabolic activation, whereas in TA1538 and TA 100 tester strains, Liv52 showed 48.4% and 47.2% of inhibition of his + revertants induced by 4-nitro-O-phenylenediamine, respectively. Further with metabolic activation in TA1538, Liv52 showed 99.8%, 99.8%, and 100% inhibition of his + revertants induced by 2-aminofluorene, 2-anthranine, and cigarette smoke condensate, respectively. In TA100 maximum of 100%, 100%, 97.7%, and 100% inhibition of his + revertants induced by 2-aminofluorene, 2-anthranine, benzo(a)pyrene, and cigarette smoke condensate, respectively, were observed. A significant enhancement of inhibition of his + revertants induced by all the above said mutagens were observed in preincubation modification method. Conclusion:Liv52 was found to be nonmutagenic in Salmonella assay, whereas manifested the antimutagenic potential both with and without metabolic activation. The enhanced antimutagenic activity of Liv52 on preincubation indicates that the antimutagenic factor(s) may be desmutagenic in nature. The exact mechanism by which Liv52 exerts antimutagenic potential is not known. The possibility of having diverse antimutagenic factors in Liv52 which act by different mechanisms are strongly indicated.

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Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays

Cited by 129 publications

References 10 publications

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Efeito genotóxico e antiproliferativo de Mikania cordifolia (L. F.) Willd. (Asteraceae) sobre o ciclo celular de Allium cepa L.

Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

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