Cytogenetic studies in Phaseolus L. (Fabaceae)

Mercado-Ruaro, Pedro; Delgado‐Salinas, Alfonso

doi:10.1590/s1415-47572000000400043

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…Glycine max and G. soja [ 68 ]; Lens culinaris and L. ervoides [ 69 ]; Medicago truncatula and M. sativa [ 70 ]. The base chromosome number in the genus Phaseolus is x = 11 [ 71 , 72 ] and most species are diploid with 2n = 22 chromosomes. Tepary bean is estimated to have a slightly larger genome (647 Mbp) than that of Lima bean ( P. lunatus , 622 Mbp) and common bean (637 Mbp) [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Gene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mapping

et al. 2016

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BackgroundCommon bean (Phaseolus vulgaris) is an important grain legume and there has been a recent resurgence in interest in its relative, tepary bean (P. acutifolius), owing to this species’ ability to better withstand abiotic stresses. Genomic resources are scarce for this minor crop species and a better knowledge of the genome-level relationship between these two species would facilitate improvement in both. High-throughput genotyping has facilitated large-scale single nucleotide polymorphism (SNP) identification leading to the development of molecular markers with associated sequence information that can be used to place them in the context of a full genome assembly.ResultsTranscript-based SNPs were identified from six common bean and two tepary bean accessions and a subset were used to generate a 768-SNP Illumina GoldenGate assay for each species. The tepary bean assay was used to assess diversity in wild and cultivated tepary bean and to generate the first gene-based map of the tepary bean genome. Genotypic analyses of the diversity panel showed a clear separation between domesticated and cultivated tepary beans, two distinct groups within the domesticated types, and P. parvifolius was confirmed to be distinct. The genetic map of tepary bean was compared to the common bean genome assembly to demonstrate high levels of collinearity between the two species with differences limited to a few intra-chromosomal rearrangements.ConclusionsThe development of the first set of genomic resources specifically for tepary bean has allowed for greater insight into the structure of this species and its relationship to its agriculturally more prominent relative, common bean. These resources will be helpful in the development of efficient breeding strategies for both species and will facilitate the introgression of agriculturally important traits from one crop into the other.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2499-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Gene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mapping

et al. 2016

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“…Chromosomal reduction is also found in three Phaseolus species with 2 n = 2 x = 20 ( Phaseolus leptostachyus Benth., Phaseolus micranthus Hook. & Arn., and Phaseolus macvaughii A. Delgado) (Mercado‐Ruaro and Delgado‐Salinas, 1998, 2000). It is still unclear what caused these chromosomal variations although chromosomal fusion is an obvious hypothesis where the chromosome number is reduced.…”

Section: Nuclear Dna Content and Chromosomes Of Legumesmentioning

confidence: 99%

Cytogenetics of Legumes in the Phaseoloid Clade

2013

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Cytogenetics played an essential role in studies of chromosome structure, behavior, and evolution in numerous plant species. The advent of molecular cytogenetics combined with recent development of genomic resources has ushered in a new era of chromosome studies that have greatly advanced our knowledge of karyotypic diversity, genome and chromosome organization, and chromosomal evolution in legumes. This review summarizes some of the achievements of cytogenetic studies in legumes in the Phaseoloid clade, which includes several important legume crops such as common bean (Phaseolus, and pigeonpea [Cajanus cajan (L.) Huth]. In the Phaseoloid clade, karyotypes are mostly stable. There are, however, several species with extensive chromosomal changes. Fluorescence in situ hybridization has been useful to reveal chromosomal structure by physically mapping transposons, satellite repeats, ribosomal DNA genes, and bacterial artificial chromosome clones onto chromosomes. Polytene chromosomes, which are much longer than the mitotic chromosomes, have been successfully found and used in cytogenetic studies in some Phaseolus and Vigna species. Molecular cytogenetics will continue to be an important tool in legume genetics and genomics, and we discuss future applications of molecular cytogenetics to better understand chromosome and genome structure and evolution in legumes.

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“…The chromosome number in this genus was first reported in 1925 when Karpetschenko (1925) showed it to be 2n = 22 for some species, including Phaseolus vulgaris L., which is one of the most important species of the genus (Mercado-Ruaro and Delgado-Salinas, 2000;Bonifácio et al, 2012). Initial work aimed to establish the basic chromosome number, which is x = 11 (Lackey, 1979;Mercado-Ruaro and Delgado-Salinas, 1998).…”

Section: Introductionmentioning

confidence: 99%

Implications of mitotic and meiotic irregularities in common beans (Phaseolus vulgaris L.)

et al. 2016

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ABSTRACT. The common bean has great social and economic importance in Brazil and is the subject of a high number of publications, especially in the fields of genetics and breeding. Breeding programs aim to increase grain yield; however, mitosis and meiosis represent under explored research areas that have a direct impact on grain yield. Therefore, the study of cell division could be another tool available to bean geneticists and breeders. The aim of this study was to investigate irregularities occurring during the cell cycle and meiosis in common bean. The common bean cultivar used was BRSMG Talismã, which owing to its high yield and grain quality is recommended for cultivation in Brazil. We classified the interphase nuclei, estimated the mitotic and meiotic index, grain pollen viability, and percentage of abnormalities in both processes. The mitotic index was 4.1%, the interphase nucleus was non-reticulated, and 19% of dividing somatic cells showed abnormal behavior. Meiosis also presented irregularities resulting in a meiotic index of 44.6%. Viability of pollen grains was 94.3%. These results indicate that the common bean cultivar BRSMG Talismã possesses repair mechanisms that compensate for changes by producing a large number of pollen grains. Another important strategy adopted by bean plants to ensure stability is the elimination of abnormal cells by apoptosis. As the common bean cultivar BRSMG Talismã is recommended for cultivation because of its good agronomic performance, it can be concluded that mitotic and meiotic irregularities have no negative influence on its grain quality and yield.

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Cytogenetic studies in Phaseolus L. (Fabaceae)

Cited by 17 publications

References 17 publications

Gene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mapping

Gene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mapping

Cytogenetics of Legumes in the Phaseoloid Clade

Implications of mitotic and meiotic irregularities in common beans (Phaseolus vulgaris L.)

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