Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)

Azevedo, Andréia Cristiane Souza; Sosa–Gómez, Daniel Ricardo; Faria, Marcos; Fungaro, Maria Helena Pelegrinelli

doi:10.1590/s1415-47572000000100010

Cited by 8 publications

(6 citation statements)

References 19 publications

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“…Mycoviral infections can be very persistent and difficult to eliminate from their hosts (Martins et al, 1999;Romo et al, 2007). Different attempts to cure fungi from viruses have been reported, e.g., cycloheximide treatments, single conidium subculture, hyphal tip transfer, incubation at low or high temperatures (Carroll and Wickner, 1995;Romo et al, 2007;Souza-Azevedo et al, 2000), but they were not always successful. These difficulties to cure fungi from viral infections, plus the lack of simple methods for the artificial inoculation of mycoviruses, have greatly hampered progress in exploring mycovirus-host interactions (Ghabrial and Suzuki, 2009).…”

mentioning

confidence: 99%

Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum

Herrero

Zabalgogeazcoa

2011

Virus Research

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Herrero N, Zabalgogeazcoa I. 2011. Mycoviruses infecting the entomopathogenic fungus Tolypocladium cylindrosporum. Virus Research 160: [409][410][411][412][413] A mixed virus infection in a strain of the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum was deduced from a study of the transmission to conidia of several double-stranded RNA (dsRNA) elements. The transmission rates of each dsRNA were different, and monosporic isolates harbouring different combinations of the original set of six dsRNAs were obtained. A 5196 bp dsRNA element was sequenced and represents the genome of Tolypocladium cylindrosporum virus 1 (TcV1), a new member of the genus Victorivirus in the Totiviridae family.This virus was transmitted to 81.4% of the conidia; in contrast, four dsRNAs of 3.1-3.7 kbp were transmitted only to 4.7% of the monosporic isolates obtained from the infected parental strain.These four dsRNAs did not show segregation during transmission, one of them was sequenced and encoded an RdRp, suggesting that the four molecules might represent the whole genome of a multipartite chrysovirus. A third possible virus with a genome of approximately 4.2 kbp was transmitted to 79.1% of the monosporic isolates produced by the infected strain. Ribavirin was used to cure T. cylindrosporum from viruses, and TcV1 was sensitive to this drug. All monosporic cultures derived from the infected strain treated with 80 and 100 µM concentrations of the drug were free of TcV1. Keywords: mycovirus, dsRNA, ribavirin, transmission, victorivirusThe ascomycete Tolypocladium cylindrosporum (Fam. Ophiocordycipitaceae) was first reported as a soil-borne species, and later as a pathogen of several species of insects, including mosquito genera like Anopheles and Aedes (Gams, 1971; Lam et al., 1988). The fungus is also pathogenic to crustaceans and arachnids like the ticks Ornithodoros erraticus and O. moubata (Herrero et al., 2011). In addition, this fungus has been isolated as an endophyte from leaves of some grasses (Sánchez Márquez et al., 2010). Other entomopathogenic fungi like Beauveria bassiana, Lecanicillium lecanii, or Metarhizium anisopliae have also been reported as endophytes and soil inhabitants, and have been tested as biological control agents for invertebrate plant pests (Vega et al., 2008).The presence of dsRNA molecules of viral origin has been reported in endophytic strains of T. cylindrosporum, and other species of entomopathogenic fungi such as B. bassiana, M. anisopliae and Paecilomyces spp. (Herrero et al., 2009; Inglis and Valadares-Inglis, 1997; Melzer and Bidochka, 1998). However, in none of these cases were these viruses classified.Mycoviral infections can be very persistent and difficult to eliminate from their hosts (Martins et al., 1999; Romo et al., 2007). Different attempts to cure fungi from viruses have been reported, e.g., cycloheximide treatments, single conidium subculture, hyphal tip transfer, incubation at low or high temperatures (Carroll and Wickner, 1995; Romo et al., 2007; Souza-Aze...

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confidence: 99%

Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum

Herrero

Zabalgogeazcoa

2011

Virus Research

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“…Strain 3398M is only infected by TcV2 and TcV3. The size of the genomes of these three viruses is similar to that of some dsRNA elements reported in other hypocrealean entomopathogenic fungi such as M. anisopliae, M. flavoviride, B. bassiana, P. amoenoroseus, or P. fumosoroseus (Bidochka et al, 2000;Dalzoto et al, 2006;Inglis and Valadares-Inglis, 1997;Melzer and Bidochka, 1998;Souza Azevedo et al, 2000).…”

Section: Discussionsupporting

confidence: 77%

“…Mycoviral infections can be very persistent, and difficult to eliminate from their hosts (Martins et al, 1999;Romo et al, 2007). Many attempts to cure fungi from viruses using different treatments have been reported, e.g., cycloheximide treatments, single conidium subculture, hyphal tip transfer, incubation at low or high temperatures (Carroll and Wickner, 1995;Marquez et al, 2007;Romo et al, 2007;Souza Azevedo et al, 2000), but they were not always successful. These difficulties to cure fungi from viral infections, and the lack of simple methods for the artificial inoculation of mycoviruses, have greatly hampered progress in exploring mycovirus-host interactions (Ghabrial and Suzuki, 2009).…”

Section: Introductionmentioning

confidence: 99%

Micovirus asociados a los hongos endofíticos y entomopatógenos. Tolypocladium cylindrosporum y beauveria bassiana

Asensio¹

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“…Fargues et al (2002) reported on the genetic variability among P. fumosoroseus isolates as a function of geographical and host insect origins. Azevedo et al (2000b) identified isolates with double stranded RNA, but they exhibited no differences in virulence from isolates with single stranded RNA. Cantone and Vandenberg (1998) considered that DNA fingerprinting with RAPD-PCR could be useful in tracking the fate of fungi released into the environment as biological control agents.…”

Section: Genetic and Molecular Studiesmentioning

confidence: 96%

Entomopathogenic Fungi for Control of Bemisia tabaci Biotype B: Foreign Exploration, Research and Implementation

Lacey¹,

Wraight²,

Kirk

2008

Classical Biological Control of Bemisia Tabaci in the United States - A Review of Interagency Research and Implementation

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Prior to the global outbreak of Bemisia tabaci type B in the early 1990s, very little attention was paid to the potential of fungal pathogens for control of this whitefly. A massive foreign exploration effort was mounted in 1991 by the USDA Agricultural Research Service to collect and develop fungi and other natural enemies of whiteflies. From 1990 until 1996, dozens of trips were made by scientists from the USDA European Biological Control Laboratory (EBCL) in Montpellier, France specifically for the purpose of finding natural enemies of whiteflies. The countries visited by EBCL ranged from European countries bordering the Mediterranean (Spain, France, Italy, Greece, Cyprus), to the Middle East (Israel, Egypt), Western Asia (Pakistan, India, Nepal), Southeast Asia (Thailand, Malaysia) and Latin America (Argentina and Brazil). Other exploratory efforts for entomopathogenic fungi within the USA and in other foreign countries (Philippines, Indonesia, Taiwan) were also undertaken by USDA-ARS personnel in Weslaco, Texas. The most prevalent fungus attacking B. tabaci in the field was Paecilomyces fumosoroseus. Fungi from whiteflies or from other insects with good activity against the silverleaf whitefly include Paecilomyces spp., Lecanicillium lecanii (=Verticillium), Beauveria bassiana and Aschersonia spp. Hundreds of isolates of these fungi were collected and shipped to the USA. Subsequent research involved screening of isolates for activity, study of factors that limited or enhanced their activity, and evaluation of candidate fungi in field and glasshouse crops. The literature on research for the development and implementation of these fungi is reviewed with recommendations for future avenues of research and development.

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Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)

Cited by 8 publications

References 19 publications

Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum

Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum

Micovirus asociados a los hongos endofíticos y entomopatógenos. Tolypocladium cylindrosporum y beauveria bassiana

Entomopathogenic Fungi for Control of Bemisia tabaci Biotype B: Foreign Exploration, Research and Implementation

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