1999
DOI: 10.1590/s1415-47571999000400010
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Abstract: Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum … Show more

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Cited by 1 publication
(4 citation statements)
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References 14 publications
(17 reference statements)
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“…Muramatsu et al (5) used tungsten particles and 285 psi nitrogen to obtain 45.8% transformation efficiency and 42.9% survival rate. However, these values as well as the number and intensity of expression units were lower than those reported by Ribeiro et al (7). These investigators obtained 68% transformation efficiency, 31.2% survival rate and an average of 100 expression units in the embryo body using gold particles, 600 mmHg vacuum and 600 psi helium pressure.…”
Section: Gfp Expressionmentioning
confidence: 69%
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“…Muramatsu et al (5) used tungsten particles and 285 psi nitrogen to obtain 45.8% transformation efficiency and 42.9% survival rate. However, these values as well as the number and intensity of expression units were lower than those reported by Ribeiro et al (7). These investigators obtained 68% transformation efficiency, 31.2% survival rate and an average of 100 expression units in the embryo body using gold particles, 600 mmHg vacuum and 600 psi helium pressure.…”
Section: Gfp Expressionmentioning
confidence: 69%
“…Efforts to improve particle dispersion by increasing the distance of DNA-coated microparticles from the embryo resulted in fewer transformed cells. Previous reports have shown that the biolistic process can be used to transfer genes to chickens in ovo (5,7,17). Li et al (17) transformed primordial chicken embryo cells using an adapted surgical syringe and were able to detect the transgene in 7.5% of the bombarded embryos.…”
Section: Gfp Expressionmentioning
confidence: 99%
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