Comparação de três testes sorológicos aplicados ao diagnóstico da infecção de caninos por Brucella canis

Azevêdo, Sérgio Santos de; Vasconcellos, Sílvio Arruda; Keid, Lara Borges; Grasso, Lília Márcia Paulin da Silva; Pinheiro, Sônia Regina; Mascolli, Roberta; Alves, Clebert José

doi:10.1590/s1413-95962004000200005

Cited by 6 publications

(6 citation statements)

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“…During this study significant statistical differences were not observed when the sex of the dog infected was compared (Table 1); similar results have been described (MORAES et al, 2002;ALMEIDA et al, 2004;CAVALCANTI et al, 2006;PORTO;PINHEIRO JUNIOR;MOTA, 2008). These results might suggest that sexual preference is not an important epidemiological factor associated with the transmission of CB.…”

supporting

confidence: 88%

“…Further, most studies of CB were done within the states of the Southeastern region of Brazil AZEVEDO et al, 2004;FERREIRA et al, 2007), with few epidemiological surveys realized in northern (AGUIAR et al, 2005), and north-eastern (CAVALCANTI et al, 2006;PORTO;PINHEIRO JUNIOR;MOTA, 2008) Brazil. There is one epidemiological survey of CB within southern Brazil (VARGAS et al, 1996).…”

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confidence: 99%

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Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

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This study evaluated the presence of circulating antibodies of anti-Brucella canis and anti-B. abortus in shelter dogs maintained in four cities of Northern Paraná. Serum samples of 100 dogs, of both sexes, from the cities of Apucarana, Arapongas, Londrina and Rolândia were collected for the determination of the serological presence of B. canis by the utilization of agarose gel immunodiffusion and B. abortus by the buffered acidified antigen assay. Only 4% (4/100) of the samples evaluated demonstrated seroreactivity to B. canis, with seropositivity varying between 4.17 -10%. However, positive samples originated only from the cities of Apucarana (4.17%; 2/48) and Londrina (10%; 2/20). Seroreactivity to B. abortus antibodies was not verified within the canine population evaluated. These results suggest that B. canis is circulating within the dog population of Northern Paraná. Key words: Canine disease, epidemiology, brucellosis, serology ResumoEste estudo avaliou a presença de anticorpos circulantes anti-Brucella canis e anti-B. abortus em cães mantidos em abrigos situados em quatro municípios da região Norte do Paraná. Amostras séricas de 100 cães, de ambos os sexos, oriundos das cidades de Apucarana, Arapongas, Londrina e Rolândia foram colhidas para determinação sorológica da presença de anticorpos contra B. canis por meio da técnica de imunodifusão em gel de agarose e de anticorpos contra B. abortus por meio da prova do antígeno acidificado tamponado. Somente 4% (4/100) das amostras avaliadas foram sororreagentes a B. canis, com soropositividade variando entre 4.17 -10%. Contudo, as amostras positivas foram oriundas somente das cidades de Apucarana (4.17%; 2/48) e Londrina (10%; 2/20). Não foram verificadas amostras sororreagentes a B. abortus na população canina avaliada. Esses resultados sugerem que B. Canis circula na população de cães da região Norte do Paraná.

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confidence: 88%

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confidence: 99%

Serological detection of Brucella canis in shelter dogs from Northern Paraná

et al. 2012

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“…The technique was conducted according to the manufacturer's recommendations using lipopolysaccharides and proteins antigens of Brucella ovis, sample Reo 198. To confirm, the same technique was applied in serum treated by 2-mercaptoetanol (AGID+2-ME) according to AZEVEDO et al (2004). For the anti-B.abortus sp.…”

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confidence: 99%

Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil

et al. 2021

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Canine brucellosisis an infectious disease caused by bacteria of the genus Brucella, with world wide distribution and zoonotic impact, and in humans and animals is a neglected disease. In the present study, the sero prevalence of B. canis and B. abortus were determined in a probabilistic sample of housed dogs from the Atlantic Rainforest area of the state of Paraíba, Brazil, and the factors associated with sero positivity. A total of 386 dogs over three months of age were used. For the search for anti-B.canis antibodies the agar gel immune diffusion test (IDGA) was used as a screening and IDGA+2ME as confirmatory test, and to search for anti-B. abortus antibodies the Rose Bengal test (RBT) test was used. Apparent and real prevalences were calculated, and robust Poisson regression was used to identify factors associated with prevalence. The real prevalence fB. Canis was 12.6% and of B. abortus was 22.8%. The factors associated with sero positivity for B. canis were age greater than 10 years (prevalence ratio; PR = 6.38; P = 0.024) and dogs reared in they ard (PR = 5.20; P = 0.035) and for B. abortus was no treplacement of water of animals everyday (PR = 1.48; P = 0.033). It can be concluded that the prevalence of B. canis and B. Abortus in the region is high, which warns to the adopting of control and prevention measures, as well as greater care in the management of animals, especially for elderly dogs.

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“…Canine brucellosis, a zoonotic disease caused by various Brucella species, is currently of great concern to public health (AZEVEDO et al, 2004), and is mainly transmitted by contact between dogs and children. Although the main etiological agent of canine brucellosis is B. canis, dogs may also be infected with B. abortus, B. suis, or B. melitensis, becoming potential sources of infection for other animals and humans (OLIVEIRA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Detection of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs

Hafemann¹,

Merlini²,

Gonçalves³

et al. 2018

Semina: Ciênc. Agrár.

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Dogs can act as intermediary hosts, reservoirs, and sentinel animals for zoonotic diseases such as brucellosis, toxoplasmosis and leptospirosis, and human contact with domestic animals can spread these infections. These diseases are globally distributed, and are a uniquely severe health issue, since they can infect a great range of animals, including humans. The purpose of this work was to determine the prevalence of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs. Blood samples were collected from 181 stray dogs and used for serological diagnosis. Of the analyzed samples, 36.46%, 16.57%, and 9.39% were positive for T. gondii, Leptospira spp., and B. canis. The results indicate that these zoonotic diseases are highly prevalent in stray dogs in the northwestern region of the state of Paraná. The high infection rates for these zoonotics in the canine population is an indication that the environment is contaminated with a variety of different microorganisms, exposing both humans and dogs to different sources of infection. Key words: Brucella canis. Dogs. Leptospira spp. Toxoplasma gondii. Public health. ResumoO contato do homem com alguns animais domésticos faz com que algumas zoonoses como brucelose, toxoplasmose e leptospirose, tornem-se infecções importantes e, com isso os cães atuam como hospedeiros intermediários, reservatórios e animais sentinelas para estas infecções. Essas enfermidades tem distribuição mundial, e é um grave problema de saúde pública, podendo infectar uma grande variedade de animais, entres os quais, se destacam o cão e o homem. O objetivo deste trabalho foi detectar a ocorrência de anticorpos contra Leptospira spp., Brucella spp. eToxoplasma gondii em cães

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Comparação de três testes sorológicos aplicados ao diagnóstico da infecção de caninos por Brucella canis

Cited by 6 publications

References 0 publications

Serological detection of Brucella canis in shelter dogs from Northern Paraná

Serological detection of Brucella canis in shelter dogs from Northern Paraná

Zoonotic smooth and rough Brucella in dogs: seroprevalence and associated factors in an Atlantic Rainforest area of the state of Paraíba, Northeastern Brazil

Detection of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs

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