Apoptosis as a prognostic marker in canine mammary tumors by TUNEL

Pereira, Kariny Sanches; Zuccari, Débora Aparecida Pires de Campos; Cury, Patrí­cia Maluf; Cordeiro, José Antônio

doi:10.1590/s1413-95962003000500007

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…44 Moreover, an increase in apoptotic index in malignant tumours, rather than in benign tumours, has been reported in dogs. 45,46 Elevation of the apoptotic index in malignant tissues in the present and previous studies may be explained by anastasis, whereby even late-stage apoptosis is reversed in cancer cells. 44 Since apoptotic cells acquire genomic instability originating from DNA fragmentation and recovery, they could be tumour repopulating cells.…”

Section: Discussionsupporting

confidence: 60%

“…Some studies have suggested that increased apoptosis is involved in aggressive behaviour, histologic grade and tumour size 44 . Moreover, an increase in apoptotic index in malignant tumours, rather than in benign tumours, has been reported in dogs 45,46 . Elevation of the apoptotic index in malignant tissues in the present and previous studies may be explained by anastasis, whereby even late‐stage apoptosis is reversed in cancer cells 44 .…”

Section: Discussionsupporting

confidence: 59%

See 1 more Smart Citation

Tumour necrosis factor‐related apoptosis‐inducing ligand (TRAIL) loss in canine mammary carcinoma

Kim

Seung

Bae

et al. 2021

Vet Comparative Oncology

View full text Add to dashboard Cite

Escaping apoptosis is a hallmark of cancer. Tumour necrosis factor-related apoptosisinducing ligand (TRAIL), a central molecule that regulates the extrinsic apoptotic pathway, has been widely investigated in human oncology; however, investigations focusing on the endogenous expression of TRAIL in canine tumours are lacking.Therefore, we aimed to examine the expression of endogenous TRAIL in canine mammary tumours and analysed its correlation with downstream molecules Fas-associated protein with death domain (FADD) and caspase-3, and to the apoptotic index. A total of 147 samples, classified as normal mammary gland (n = 9), mammary adenoma (n = 30), low-grade carcinoma (n = 42) and high-grade carcinoma (n = 66), were included in the immunohistochemical analyses, and 43 samples with sufficient levels of RNA were analysed via RNA in situ hybridization and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. In immunohistochemistry, TRAIL protein expression was significantly decreased in high-grade carcinoma compared to those in normal mammary gland and adenoma, with similar downregulation of TRAIL mRNA expression. Also, FADD and caspase-3 expression positively correlated with TRAIL expression. However, the apoptotic index was paradoxically elevated in high-grade tumours. Overall, these results suggest that the loss of TRAIL accompanied by dysregulation of TRAIL-induced extrinsic apoptotic pathway molecules could affect malignant progression of canine mammary tumours.

show abstract