Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae

Pantano, T; Mello, Marco Roberto Bourg de; Garcia, José Fernando; Ho, Linda Lee; Visintin, José Antônio

doi:10.1590/s1413-95962000000300012

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Cocero, et al [29] obtained better embryo viability rates after cryopreservation of ovine embryos with ethylene glycol instead of glycerol. Similar results were reported by Pantano, et al [30], with a superiority of ethylene glycol in comparison with propylene glycol and glycerol for freezing mouse embryos. Conversely, Martinez and Matkovic [31] compared embryo development and hatching rates of ovine embryos, and did not find statistical differences between either cryoprotectant.…”

Section: Resultssupporting

confidence: 90%

Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

et al. 2012

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The objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from M0 were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5°C under an atmosphere of 5% CO(2) with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 ± 4.8 and 57.3 ± 4.8, respectively, mean ± SEM), or among M0 (62.3 ± 5.7%), M3 (56.9 ± 6.0%), and M6 (66.5 ± 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture.

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Section: Resultssupporting

confidence: 90%

Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

et al. 2012

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“…Selain itu dalam penelitian ini juga terdapat sejumlah embrio yang hilang pada saat thawing (sekitar 14,7%). Kehilangan embrio pada penelitian ini tidak berbeda dengan hasil penelitian yang dilakukan oleh Pantano et al (2000) yang menunjukkan embrio yang hilang pada saat thawing mencapai 1,7% sampai 13,3%. Kehilangan embrio bisa disebabkan oleh embrio yang menempel/lengket pada dinding straw sehingga sulit dilepaskan pada saat thawing.…”

Section: Diskusiunclassified

Kualitas, Kemampuan Implantasi dan Viabilitas in-vivo Embrio Mencit (Mus muculus) Galur Swiss Webster Setelah Pembekuan Dengan Metode Vitrifikasi

Madihah¹,

Kusumaningtyas²,

Boediono³

et al. 2019

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.

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Effect of Prostatic Fluid and Different Cryoprotectants on post-thaw Dog Spermatozoa

Kusum¹,

Gupta²,

Roy³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae

Cited by 3 publications

References 10 publications

Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

Kualitas, Kemampuan Implantasi dan Viabilitas in-vivo Embrio Mencit (Mus muculus) Galur Swiss Webster Setelah Pembekuan Dengan Metode Vitrifikasi

Effect of Prostatic Fluid and Different Cryoprotectants on post-thaw Dog Spermatozoa

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