Parâmetros utilizados na avaliação do sêmen congelado ovino para inseminação laparoscópica

Luz, S. L. N.; Neves, Jairo Pereira; Gonçalves, Paulo Bayard Dias

doi:10.1590/s1413-95962000000200010

Cited by 3 publications

(3 citation statements)

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“…For year round lamb production, reproduction management i n the form of induction of estrus by hormonal means has been used with success and has resulted in high reproductive rates in sheep (Luz et al, 2000).…”

Section: Lntrod Uctionmentioning

confidence: 99%

Reproductive Behavior and Efficiency in Pelibuey Ewes Treated with FGA-PMSG and Bred by Mounting or Laparoscopic Intrauterine Insemination

Quintero-Elisea¹,

Vázquez-Armijo

Cienfuegos-Rivas

et al. 2010

Journal of Applied Animal Research

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Section: Lntrod Uctionmentioning

confidence: 99%

Reproductive Behavior and Efficiency in Pelibuey Ewes Treated with FGA-PMSG and Bred by Mounting or Laparoscopic Intrauterine Insemination

Quintero-Elisea¹,

Vázquez-Armijo

Cienfuegos-Rivas

et al. 2010

Journal of Applied Animal Research

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“…The thermoresistance test (TTR) allows determination of the number of spermatozoa active after the test and the fertilization rate, thus enabling the elimination of inadequate semen samples, that is, those with a final motility lower than 20% at the end of incubation [14]. However, previous studies have stated that there is no correlation between this test and the pregnancy rate [2].…”

Section: Discussionmentioning

confidence: 99%

Action of Cholesterol-Loaded Cyclodextrin on Viability of Ram Semen

Silva

Almada

Lima

et al. 2019

Acta Scientiae. Vet.

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Background: Studies report that cyclodextrins have the property of carrying cholesterol to the membrane, but in some cases can also remove this cholesterol from the plasma membrane. The mechanism of action of CLC is not well understood, however, it seems to involve sperm protection during the freezing and thawing process. Studies show that its use enhancing increased osmotic tolerance and reduced premature sperm capacitation reaction. In this sense, studies report that cyclodextrins have the property of carrying cholesterol to the membrane, but in some cases can also remove this cholesterol from the plasma membrane. Improvements were reported in the sperm parameters of buffaloes, bulls, stallions and sheep. Ram naturally present less lipids in their membrane, on average 27%, while bulls have 31%, rabbits 62%, and humans 50%. The aim of the present study was to evaluate the use of cholesterol-loaded cyclodextrin (CLC), a commercial diluent, in the kinetics and viability of frozen and thawed ram spermatozoa. Materials, Methods & Results:Five ejaculates, from five rams of Dorper breed were collected and divided into three groups: control, 1 mg CLC and 2 mg CLC. Semen was diluted in different concentrations of CLC (0, 1, and 2 mg/120×10 6 spermatozoa), and incubated at room temperature (21°C) for 10 min. Samples were conditioned in 0.5 mL straws and incubated at 5°C for 4 h, exposed to LN 2 vapor for 10 min and storing a cryogenic container. The parameters as spermatic kinetics, plasma membrane, acrosomal membrane (MPAI, %), and intracellular levels of superoxide anion (O 2-) were evaluated. Sperm progressive motility (PM), rapid spermatozoa percentage (RAP), linearity (LIN, %), average path velocity (VAP, μm/s) and MPAI (%) were more satisfactory with the use of 1 mg compared to 2 mg (P < 0.05). In addition, 1 mg CLC showed decreased levels of superoxide anion formation (O 2-), a free radical detrimental to spermatozoa (P < 0.05). The use of 2 mg of CLC reduce VAP (P < 0.05) and did not have any beneficial effect on the evaluated parameters. Discussion: Authors did not observe improvement in the parameters of progressive motility when using 1 mg of CLC in goat semen and 2 mg in bull semen with the slow freezing protocol. This differs from our work, as we found that 1 mg of CLC improved the PM parameters, but not at the concentration of 2 mg CLC. Additionally, authors verified that cyclodextrin at 3 mg concentration was effective in protecting the sperm against the deleterious effects of H 2 O 2 . They obtained superior plasma membrane motility, viability, and integrity of the CLC-treated samples compared to the control group. The superoxide anion (O 2-) is a free radical formed from molecular oxygen by the addition of an electron. It is generated spontaneously, mainly in the membrane of the mitochondria, by the respiratory chain and by flavoenzimes, lipoxygenases, and cicloxygenases. In our study, we found a difference between the study group with 1 mg CLC and the control group. Thus, we suggest that CLC may have a ...

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“…A inexistência de técnicas rápidas, eficazes e seguras para se avaliar a capacidade fecundante da célula espermática caprina, antes e após o processo de congelamento, representa um dos grandes desafios para se avaliar a eficiência das mmmmmmm biotecnologias utilizadas na reprodução animal (Luz et al, 2000), como a Inseminação Artificial (IA), Produção de Embriões in Vitro (PIV) e Intracytoplasmic Sperm Injection (ICSI). O *Autor para correspondência: E-mail: diasjuliovet@yahoo.com.br Teste de Ligação do Espermatozoide à Membrana Perivitelina da Gema de Ovo permite verificar a capacidade fecundante da célula espermática, e assim, é considerado um valioso teste na análise da eficácia do processo de criopreservação do sêmen (Barbato et al, 1998).…”

Section: Introductionunclassified

Sperm-perivitelline membrane egg binding assay after addition of Ringer Lactate, Sodium Citrate 2.92 % and TRIS Solution in thawed goat semen

Dias

2014

AVB

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RESUMO - (CBRA,1998). A capacidade de ligação dos espermatozoides à membrana perivitelina da gema do ovo foi semelhante entre os tratamentos (P>0,05), mas foi observada correlação positiva entre a motilidade espermática e a capacidade de ligação (P<0,05). A diluição do sêmen criopreservado com soluções isoosmóticas após o descongelamento pode ser útil na manutenção da qualidade espermática e no aumento do volume existente a fim de otimizar a realização de testes e biotecnologias reprodutivas in vitro. Palavras-chave: bode, diluente, espermatozoide.ABSTRACT -The present study was verified by testing for binding of sperm to the perivitelline membrane of the egg yolk fertilizing capacity of these cells after thawing and addition of Ringer Lactate, Sodium Citrate 2.92% and TRIS Solution. The semen of animals was collected and performed the standard procedures of analysis and cryopreservation seminal. After thawing was added the solutions Ringer Lactate, Sodium Citrate 2.92% and TRIS, which contained protective substances to sperm and performed the tests in vitro SpermPerivitelline Membrane Egg Binding and Morphology. The seminal parameters of fresh semen were recommended according to current legislation. The binding capacity of the sperm membrane perivitelline egg yolk was similar between treatments (P>0,05), but there was a positive correlation between sperm motility and binding capacity (P<0,05). Thus, the dilution of semen cryopreserved with solutions isoosmóticas after thawing can be useful in maintaining the quality of sperm and increase existing volume to optimize the testing and reproductive biotechnologies in vitro.

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Parâmetros utilizados na avaliação do sêmen congelado ovino para inseminação laparoscópica

Cited by 3 publications

References 0 publications

Reproductive Behavior and Efficiency in Pelibuey Ewes Treated with FGA-PMSG and Bred by Mounting or Laparoscopic Intrauterine Insemination

Reproductive Behavior and Efficiency in Pelibuey Ewes Treated with FGA-PMSG and Bred by Mounting or Laparoscopic Intrauterine Insemination

Action of Cholesterol-Loaded Cyclodextrin on Viability of Ram Semen

Sperm-perivitelline membrane egg binding assay after addition of Ringer Lactate, Sodium Citrate 2.92 % and TRIS Solution in thawed goat semen

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