Influência de diferentes diluentes e temperaturas de refrigeração sobre a qualidade de sêmen suíno

Kotzias-Bandeira, Edna

doi:10.1590/s1413-95961999000400005

Cited by 3 publications

(1 citation statement)

References 1 publication

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“…Four ejaculates were obtained from each boar at a regime of one ejaculate each second day. Ejaculates were diluted with Gold Life (Schippers, Europe BV, AH, The Netherlands) to a final concentration of 350 × 10 6 sperm/ml, equilibrated to approximately 38°C and used immediately.…”

Section: Methodsmentioning

confidence: 99%

Effect of sexual steroids on boar kinematic sperm subpopulations

Ayala¹,

Martínez

2017

Cytometry Pt A

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Here, we show the effects of sexual steroids, progesterone, testosterone, or estradiol on motility parameters of boar sperm. Sixteen commercial seminal doses, four each of four adult boars, were analyzed using computer assisted sperm analysis (CASA). Mean values of motility parameters were analyzed by bivariate and multivariate statistics. Principal component analysis (PCA), followed by hierarchical clustering, was applied on data of motility parameters, provided automatically as intervals by the CASA system. Effects of sexual steroids were described in the kinematic subpopulations identified from multivariate statistics. Mean values of motility parameters were not significantly changed after addition of sexual steroids. Multivariate graphics showed that sperm subpopulations were not sensitive to the addition of either testosterone or estradiol, but sperm subpopulations responsive to progesterone were found. Distribution of motility parameters were wide in controls but sharpened at distinct concentrations of progesterone. We conclude that kinematic sperm subpopulations responsive to progesterone are present in boar semen, and these subpopulations are masked in evaluations of mean values of motility parameters. © 2017 International Society for Advancement of Cytometry.

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Section: Methodsmentioning

confidence: 99%

Effect of sexual steroids on boar kinematic sperm subpopulations

Ayala¹,

Martínez

2017

Cytometry Pt A

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Cysteine addition on short-term cooled boar semen preservation and its relationship with swine field fertility

Severo

Pereira

et al. 2011

Pesq. Vet. Bras.

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Artificial insemination is routinely used in the swine industry to reduce the costs of production through to increase the efficiency of the refrigerated boar semen process. The objective of this study was to evaluate the effect of different levels of cysteine (CYS) added to the Beltsville Thawing Solution (BTS) extender semen during cooling for up to 72 hours. Ejaculated from three boars were collected with the gloved-hand technique and semen aliquots were diluted in BTS as follow: BTS only (BTS), BTS + 0.1mM cysteine (CYS0.1), BTS + 0.5mM cysteine (CYS0.5), BTS + 1.0mM cysteine (CYS1.0), BTS + 2.5mM cysteine (CYS2.5), BTS + 5.0mM cysteine (CYS5.0), BTS + 10.0mM cysteine (CYS10.0), and BTS + 20.0mM cysteine (CYS20.0). Evaluation of sperm integrity were analyzed using 0.5mg/ml propidium iodide (plasma membrane), 100µg/ml isothiocynate-conjugated Pisum sativun agglutinin (acrosomal membrane) and 153µM 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide (mitochondria potential) after semen dilution at specific times (0, 24, 48 and 72 hours). Additionally, we also evaluated the effects of 5.0 mM CYS addition in the BTS extender on the maintenance of sperm quality and their influence on fertility in the swine production. After artificial insemination, animals were evaluated based on the estrous return and the number of piglet's born. Cysteine at concentrations of 10.0 and 20.0mM resulted in more pronounced reductions even at the time zero. Semen viability decreased to levels below 10% at these high levels of CYS in the first 24 hour of storage at 17ºC. At the end of the storage time, less than 65% of sperm cells had intact plasma membrane in all groups. The sperm viability decreased significantly when the semen was added at high concentrations of CYS (time "0"; CYS10.0 and CYS20.0; p<0.05), when compared to the other CYS concentrations. The BTS (10.20±0.39) treated group showed a lower rate of estrus return when compared to other (BTSCYS; 86.05±039), and it showed also the highest total number of piglets borne per treatment (12.71±3.38 vs. 9.00±3.38, respectively). In conclusion, the addition of CYS in the BTS semen extender did not maintain spermatic viability of boar cooled spermatozoa and it results in a higher percentage of return to estrus and lower number of piglets borne.

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Viabilidade de sêmen suíno armazenado a 5°C de acordo com a taxa de resfriamento e incubação prévia

et al. 2005

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Viability of swine semen stored at 5ºC according to the cooling rate and previous incubation RESUMO MOT, ACN and MI between T1 and T3 and also between T2 and T4. Storage at 17ºC (T1 and T3) presented better MOT and MI (P<0.05) Foram realizados dois experimentos para avaliar o efeito da incubação prévia e da taxa de resfriamento sobre a viabilidade de sêmen suíno resfriado. Doses de sêmen de 100ml, com três bilhões de espermatozóides, foram armazenadas a 17ºC ou 5ºC. Foram avaliados os percentuais de motilidade (MOT), de acrossomas normais (ACN) e de membranas íntegras (MI

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Influência de diferentes diluentes e temperaturas de refrigeração sobre a qualidade de sêmen suíno

Cited by 3 publications

References 1 publication

Effect of sexual steroids on boar kinematic sperm subpopulations

Effect of sexual steroids on boar kinematic sperm subpopulations

Cysteine addition on short-term cooled boar semen preservation and its relationship with swine field fertility

Viabilidade de sêmen suíno armazenado a 5°C de acordo com a taxa de resfriamento e incubação prévia

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