Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Abstract

Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1 st , 2007 to June 31 st , 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.

Screening of the production of metallo-beta-lactamases in multidrug-resistant Pseudomonas aeruginosa isolates existing carbapenemaze activity from Intensive Care Units.

“…P. aeruginosa ile enfekte olan hastaların fatalite oranları gram-negatif herhangi bir başka ajanla enfekte hastalara göre daha yüksektir (15). Hastanede uzun süre yatmak ve bu süre içerisinde kateter ya da mekanik ventilatör gibi invaziv girişimlere maruz kalmak, yoğun bakım ünitelerini P. aureginosa yayılımı için oldukça elverişli yerler haline getirmektedir (16).…”

Occurrence of carbapenem multidrug-resistant Pseudomonas aeruginosa carrying blaVIM metallo-β-lactamases and their biofilm phenotypes in Al-Azhar University Hospita

“…PCR amplification was accomplished using specified primers (Table 1), using the thermocycler (Personal Thermocycler Model, Biometra, Germany). An amplification program for the detection of bla IMP-1 and bla VIM-1 genes was conducted according to Rodrigues et al (2011) and Shibata et al (2003).…”

Antibiotic resistance patterns of efflux pumps MexAB-Opr M in pathogenic Pseudomonas aeruginosa isolates

“…Pseudomonas aeruginosa is a pathogenic, Gram-negative, Opportunist, movable and mandatory aerobic bacteria. Occasionally, the formation of lethal infections in their hosts, can be a most important factors causing secondary Hospital-acquired infections in patients with burns and ICU (1,2). Pseudomonas infections are commonly reported associated to infections, urinary tract infections and pulmonary diseases such as cystic brosis (3).…”