Biofilms: microbes and disease

Aparna, M; Yadav, Sarita

doi:10.1590/s1413-86702008000600016

Cited by 173 publications

(152 citation statements)

References 23 publications

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“…16 There are various methods for biofilm detection. [7][8][9][10][11][12] In this study we evaluated 110 isolates by three screening methods for their ability to form biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…6 There are various methods to detect biofilm production. These include the Tissue Culture Plate (TCP), 7 Tube method (TM), 8 Congo Red Agar method (CRA), 9 bioluminescent assay, 10 piezoelectric sensors, 11 and fluorescent microscopic examination. 12 We screened 110 organisms by three different methods, which could be used in a routine clinical laboratory, for determining their ability to form biofilm.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of different detection methods of biofilm formation in the clinical isolates

Hassan¹,

Usman²,

Kaleem³

et al. 2011

Braz J Infect Dis

215

199

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BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard microbiological procedures. Biofilm detection was tested by TCP, TM and CRA. Antibiotic susceptibility test of biofilm producing bacteria was performed by using the Kirby-Bauer disc diffusion technique according to CLSI guidelines. RESULTS: The TCP method was considered to be superior to TM and CRA. From the total of 110 clinical isolates, TCP method detected 22.7% as high, 41% moderate and 36.3% as weak or non-biofilm producers. We have observed higher antibiotic resistance in biofilm producing bacteria than non-biofilm producers. CONCLUSION: We can conclude from our study that the TCP method is a more quantitative and reliable method for the detection of biofilm forming microorganisms as compared to TM and CRA methods, and it can be recommended as a general screening method for detection of biofilm producing bacteria in laboratories

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“…16 There are various methods for biofilm detection. [7][8][9][10][11][12] In this study we evaluated 110 isolates by three screening methods for their ability to form biofilms.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of different detection methods of biofilm formation in the clinical isolates

Hassan¹,

Usman²,

Kaleem³

et al. 2011

Braz J Infect Dis

215

199

View full text Add to dashboard Cite

show abstract

“…[1] Although seemingly trivial at that time, this discovery has had a profound impact on our understanding of the pathogenesis and treatment of bacterial infections. [2][3][4][5] Today, treating biofilm infections is one of the major challenges the medical community is facing and is expected to remain so for many years to come.…”

Section: Introductionmentioning

confidence: 99%

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Forier

Raemdonck

Smedt

et al. 2014

Journal of Controlled Release

360

320

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Biofilms are matrix-enclosed communities of bacteria that show increased antibiotic resistance and the capability to evade the immune system. They can cause recalcitrant infections which cannot be cured with classical antibiotic therapy. Drug delivery by lipid or polymer nanoparticles is considered a promising strategy for overcoming biofilm resistance. These particles are able to improve the delivery of antibiotics to the bacterial cells, thereby increasing the efficacy of the treatment. In this review we give an overview of the types of polymer and lipid nanoparticles that have been developed for this purpose. The antimicrobial activity of nanoparticle encapsulated antibiotics compared to the activity of the free antibiotic is discussed in detail. In addition, targeting and triggered drug release strategies to further improve the antimicrobial activity are reviewed. Finally, ample attention is given to advanced microscopy methods that shed light on the behavior of nanoparticles inside biofilms, allowing further optimization of the nanoformulations. Lipid and polymer nanoparticles were found to increase the antimicrobial efficacy in many cases. Strategies such as the use of fusogenic liposomes, targeting of the nanoparticles and triggered release of the antimicrobial agent ensured the delivery of the antimicrobial agent in close proximity of the bacterial cells, maximizing the exposure of the biofilm to the antimicrobial agent. The majority of the discussed papers still present data on the in vitro anti-biofilm activity of nanoformulations, indicating that there is an urgent need for more in vivo studies in this field.

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“…Transitioning from acute to chronic infection is frequently associated with biofilm formation. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection (6). Bacterial attachment is mediated by fimbriae, pilli, flagella and EPS that from a bridge between bacteria and the biofilm (7).…”

Section: Introductionmentioning

confidence: 99%

Bacterial Biofilms; Links to Pathogenesis and Résistance Mechanism 

Bekele¹,

Abay²,

Gelaw³

et al. 2018

Preprint

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Biofilms are usually defined as surface-associated microbial communities, surrounded by an extracellular polymeric substance matrix. There are three major steps that are observed in biofilm formation: initial attachment events, microcolony formation and construction of mushroom-like structure with secretion of extracelluar polymeric substances. These substances can be considered a mechanism to protect the bacterial community from external insults.Biofilms, significantly increase the ability of the pathogen to evade both host defenses and antibiotics and they are being implicated in the pathogenesis and also clinical manifestation of Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Biofilms: microbes and disease

Cited by 173 publications

References 23 publications

Evaluation of different detection methods of biofilm formation in the clinical isolates

Evaluation of different detection methods of biofilm formation in the clinical isolates

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Bacterial Biofilms; Links to Pathogenesis and Résistance Mechanism

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Biofilms: microbes and disease

Cited by 173 publications

References 23 publications

Evaluation of different detection methods of biofilm formation in the clinical isolates

Evaluation of different detection methods of biofilm formation in the clinical isolates

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Bacterial Biofilms; Links to Pathogenesis and R&eacute;sistance Mechanism&nbsp;

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Bacterial Biofilms; Links to Pathogenesis and Résistance Mechanism