The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques

Abstract

Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients with haematological disorders were tested for specific antibodies and viral DNA through the use of ELISA and PCR techniques. Evidence of parvovirus B19 infection was found in 23/79 (29.1%) patients by demonstrating viral DNA and/or specific IgM antibody. B19 infection was established in 3 of 11 patients with chronic myeloid leukemia, in 3 of 11 acute myeloid leukemia, in 2 of 11 patients with multiple myeloma, in 3 of 8 patients with Hodgkin's lymphoma, in 5 of 10 patients with non-Hodgkin's lymphoma, in 1 of 6 patients with myelodysplastic syndrome, in 4 of 11 patients with chronic lymphocytic leukemia, and in 2 of 11 patients with acute lymphocytic leukemia. In 4 of 23 positive patients, only parvovirus B19 DNA could be detected, while 7 patients were tested positive for both parvovirus B19 DNA and specific IgM. Nine patients were tested positive for both B19 DNA and specific IgG. In the remaining 3 positive patients only specific IgM could be detected. Due to the discrepancies between DNA and IgM results, the diagnostic procedures should include a search for specific DNA by PCR methods if specific IgM has been found to be negative.

Clinical and hematological study for Parvovirus b19 infection in children with acute leukemia

“…Again, depending on DNA testing only would lead to missing the diagnosis in theses cases. It is clear that both DNA and serology are needed to diagnose parvovirus B19 infections in immunocompromised children (Us et al. , 2007).…”

Investigation of Parvovirus B19 IgM and IgG Positivity Rates in Pediatric Hematology Patients

“…Both parvovirus B19 specific IgM antibody and viral DNA detection have been recommended in the literature due to the limited diagnostic efficacy of each method. 17,18 The two methods have been shown to be complementary methods to each other for more precise diagnosis of acute parvovirus B19 infections. 17,18 Although a healthy control group is lacking in the present study, we could compare the rates of parvovirus IgM and IgG seropositivity with pediatric patients without hematological disorders.…”

“…17,18 The two methods have been shown to be complementary methods to each other for more precise diagnosis of acute parvovirus B19 infections. 17,18 Although a healthy control group is lacking in the present study, we could compare the rates of parvovirus IgM and IgG seropositivity with pediatric patients without hematological disorders. To the best of our knowledge, comparison between those groups has been rarely studied.…”

The Seropositivity of Parvovirus B19 Among Kidney Transplant Recipients

“…This seropositivity of PV-B19 IgM refers to acute infection. It is known that the PV-B19 infection is either due to reactivation of a latent infection in a general immunosuppression situation (18) or it is the result of a prolonged primary infection in an immunocompromised individual (19,20) . These findings are attributed mainly to reduced RTR immunity by the use of immunosuppressive regimen leading to human PV-B19 reactivation and rapid replication causing the infection in patients.…”