Role of the Parasight-F test in the diagnosis of complicated Plasmodium falciparum malarial infection

Arora, Sandeep; Gaiha, M; Arora, Anju

doi:10.1590/s1413-86702003000500008

Cited by 11 publications

(6 citation statements)

References 8 publications

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“…The accuracy of the test was 89% and I-index 0.77 in comparison with gold standard. The above results are comparable to other studies 11,12 Out of 262 microscopy positive children, 21 were found to be negative on RDT and the possible reason for this false negativity may be occasional failure of rapid test to detect high parasite densities as recorded earlier. [10][11][12] Pf HRP-2 deleted mutants have been reported but their prevalence is not known.…”

Section: Discussionsupporting

confidence: 90%

“…The above results are comparable to other studies 11,12 Out of 262 microscopy positive children, 21 were found to be negative on RDT and the possible reason for this false negativity may be occasional failure of rapid test to detect high parasite densities as recorded earlier. [10][11][12] Pf HRP-2 deleted mutants have been reported but their prevalence is not known. 7 Of the 270 children, who were positive on RDT, 241 were positive on microscopy and 29 children were negative on microscopic examination.…”

Section: Discussionsupporting

confidence: 90%

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COMPARATIVE STUDY OF RDTS v/s MICROSCOPY FOR THE DIAGNOSIS OF MALARIA IN CHILDREN

Yadav¹,

Singh²,

Singh³

et al. 2014

jemds

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Malaria presents a diagnostic challenge in most tropical countries. Rapid detection of the malaria parasite and early treatment of infection still remain the most important goals of disease management. Therefore, performance characteristics of the indigenous RDTs was determined among children with suspected malaria fever attending pediatrics OPD or admitted in indoor of UP RIMS n R Saifai central India, to assess whether this rapid diagnostic test (RDT) could be used for diagnosis of malaria and results were compared with Gold Standard microscopy test. We also assessed the logical utilization of RDTs to monitor treatment outcome. MATERIALS AND METHODS: 03 months to 12 years old children who were presented with acute fever without any focus to the OPD or IPD of our department from May 2011 to April 2013 were selected for the study. A finger prick blood sample was collected from each clinically suspected case of malaria to prepare blood smear and for testing with the RDT after taking informed consent. The blood smears were read by an experienced microscopist blinded to the RDT results and clinical status of the subjects. The figures for specificity, sensitivity, accuracy and predictive values were calculated using microscopy as gold standard. RESULTS: Analysis revealed that overall sensitivity, specificity and accuracy of the RDT were approx. 90%, while RDT is useful to confirm the diagnosis of new symptomatic cases of suspected malaria infection, the persistence of parasite antigen leading to false positives even after clearance of asexual Parasitaemia has limited its utility as a prognostic tool. The study showed that RDTs was easy to use, reliable and cheap for diagnosing new malaria cases, and is an appropriate test for the use in the fields and remote areas.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

COMPARATIVE STUDY OF RDTS v/s MICROSCOPY FOR THE DIAGNOSIS OF MALARIA IN CHILDREN

Yadav¹,

Singh²,

Singh³

et al. 2014

jemds

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“…Other studies also reported high sensitivity ranges from 100% to 96% [7,8]. Which is higher than the threshold of 95% recommended by the World Health Organization (WHO) some other studies reported from 89% to 100% [9][10][11]? The specificity in these studies range from 80% to 100%.…”

Section: Resultsmentioning

confidence: 96%

Comparison of Microscopic Determination and Rapid Diagnostic Tests (RDTs) in the Detection of Plasmodium Infection

Naz¹,

Khan²,

Farooqui³

et al. 2016

SJAMS

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Malaria is a protozoan disease caused by the parasites of the genus Plasmodium; Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum, and Plasmodium ovale. Microscopy is the gold standard for the laboratory diagnosis of malaria parasite but its turnaround time is much more than that of RDT and it requires adequate training. RDTs are alternative diagnostic methods because they are quick and easy to carry out. We studied 500 blood samples of patients presented with sign and symptoms of malaria from OPD and various wards of SHKM GMC Nalhar, from June 2015 to May 2016. All of the samples obtained were first tested by RDTs, and then the same samples were used to make peripheral blood film. RDT have more sensitivity than PBF. Specificity and PPV of rapid card test were 93.6%, 87.2% and sensitivity and NPV were 91.3%, 95.7%. In case of outdoor fields activity peripheral regions RDT is a good option. We recommended that RDT in conjunction with microscopy should improve the diagnosis of malaria.

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“…The performance of different diagnostic techniques depends on the malaria species, the parasite density, previous treatment, gametocytaemia and the quality of the diagnostic method [20]. Test sensitivity and specificity, as traditionally defined, are thus purely theoretical concepts, not necessarily established in the conditions where the test is actually used.…”

Section: Introductionmentioning

confidence: 99%

True versus Apparent Malaria Infection Prevalence: The Contribution of a Bayesian Approach

et al. 2011

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AimsTo present a new approach for estimating the “true prevalence” of malaria and apply it to datasets from Peru, Vietnam, and Cambodia.MethodsBayesian models were developed for estimating both the malaria prevalence using different diagnostic tests (microscopy, PCR & ELISA), without the need of a gold standard, and the tests' characteristics. Several sources of information, i.e. data, expert opinions and other sources of knowledge can be integrated into the model. This approach resulting in an optimal and harmonized estimate of malaria infection prevalence, with no conflict between the different sources of information, was tested on data from Peru, Vietnam and Cambodia.ResultsMalaria sero-prevalence was relatively low in all sites, with ELISA showing the highest estimates. The sensitivity of microscopy and ELISA were statistically lower in Vietnam than in the other sites. Similarly, the specificities of microscopy, ELISA and PCR were significantly lower in Vietnam than in the other sites. In Vietnam and Peru, microscopy was closer to the “true” estimate than the other 2 tests while as expected ELISA, with its lower specificity, usually overestimated the prevalence.ConclusionsBayesian methods are useful for analyzing prevalence results when no gold standard diagnostic test is available. Though some results are expected, e.g. PCR more sensitive than microscopy, a standardized and context-independent quantification of the diagnostic tests' characteristics (sensitivity and specificity) and the underlying malaria prevalence may be useful for comparing different sites. Indeed, the use of a single diagnostic technique could strongly bias the prevalence estimation. This limitation can be circumvented by using a Bayesian framework taking into account the imperfect characteristics of the currently available diagnostic tests. As discussed in the paper, this approach may further support global malaria burden estimation initiatives.

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Role of the Parasight-F test in the diagnosis of complicated Plasmodium falciparum malarial infection

Cited by 11 publications

References 8 publications

COMPARATIVE STUDY OF RDTS v/s MICROSCOPY FOR THE DIAGNOSIS OF MALARIA IN CHILDREN

COMPARATIVE STUDY OF RDTS v/s MICROSCOPY FOR THE DIAGNOSIS OF MALARIA IN CHILDREN

Comparison of Microscopic Determination and Rapid Diagnostic Tests (RDTs) in the Detection of Plasmodium Infection

True versus Apparent Malaria Infection Prevalence: The Contribution of a Bayesian Approach

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