Expansion of <FONT FACE=Symbol>gd</font><FONT FACE=Symbol> </font>T cells in patients infected with cutaneous leishmaniasis with and without glucantime therapy

Abstract

The expansion of gammadelta T cells in patients with active cutaneous leishmaniasis, with or without glucantime therapy, was investigated. Twenty patients with local cutaneous leishmaniasis including glucantime-treated (n=10) and untreated (n=10) patients were selected. The controls were healthy individuals (n=10) living in endemic areas. Whole blood was obtained and the T cell subpopulations were analyzed by flow cytometry. Significantly more gammadelta CD(3)(+) T cells were observed in untreated patients (15.9% +/-5.9), when compared with glucantime-treated patients (4.6% +/-1.4) and controls (5.3% +/-2.3). On the other hand, when the percentages of alphabeta CD(3)(+) T-cells were analyzed different results were obtained. A significant increase in alphabeta T cells was seen in glucantime-treated patients (62.4% +/-7.6), when compared to the untreated patients (55.7% +/-5.5) and controls (55.1% +/-9.6). The percentage of total CD(3)(+) T cells was statistically greater in both glucantime-treated (68.8% +/-7.4) and untreated patients (73.4% +/-5.9) when compared to the controls (61% +/-10.3). These results are consistent with previous results on the expansion of gammadelta T cells during the course of cutaneous leishmaniasis. They also indicate that glucantime therapy can reverse the expansion of gammadelta T cells and as a result increase the percentages of alphabeta ab CD(3)(+) T cells.

Distinct hepatic myeloid and lymphoid cell repertoires are associated with susceptibility and resistance toAscarisinfection

“…Little is known about the roles of these innate lymphocytes in Ascaris infection, but they are implicated in immunity against other parasites. γδ T cells are found in elevated numbers in the blood of patients with cutaneous leishmaniasis (Darabi et al ., 2002 ), Toxoplasma gondii (Prigione et al ., 2006 ) and Schistosoma infections (Schwartz et al ., 2014 ) and in the mesenteric lymph nodes of Schistosoma -infected mice (Yu et al ., 2014 ). γδ T cells numbers also increase after the acute phase of Plasmodium infection (Mamedov et al ., 2018 ) and are required for the induction of immunity against Plasmodium in vaccination studies in mice (Zaidi et al ., 2017 ).…”

The flow-cytometry-based evaluation of cellular immunity in cases of cutaneous leishmaniasis and healthy controls from the endemic area in southern Israel

“…It would also clearly be useful to know how the early treatment of CL affects the development of the beneficial Th1 response (Frankenburg et al, 1993b;Darabi et al, 2002;Machado et al, 2002). If chemotherapy given early in the disease process leads to parasite death, antigen release and subsequent immunization, it should be possible to prevent the development of clinical disease (i.e.…”

“…There is some worrying evidence indicating that chemotherapy may delay or even prevent the development of protective immunity (Frankenburg et al, 1993b;Darabi et al, 2002). This may explain why, in cases of CL caused by L. braziliensis, Machado et al (2002) were unable to prevent the ulceration of the developing lesions by the early administration of antimonials.…”