Extraction of γ-oryzanol from rice bran

Abstract

ABSTRACTγ -oryzanol, a phytochemical, has antioxidant activities and potential health benefits. In this study, we aimed to develop a method for extracting high amounts of γ -oryzanol from rice bran and for verifying its stability in different solvents. Of the 5 methods under investigation, the one that yielded the highest γ -oryzanol content was further improved by using a central composite rotational design (DCCR -2 4 , including 8 tests in axial conditions and 4 central points, totaling 28 trials). The parameters under study were temperature, time, volume, and the ratio of hexane:isopropanol. High γ -oryzanol content (13.98 mg . g -1 ) was obtained by using the following extraction conditions: 40° C, 40 min, and 75 mL hexane:isopropanol (1:3). The limits of detection and quantification of the method were 0.9 µg g -1 and 31 µg g -1 , respectively. The instrumental precision was 0.004%, the repeatability (CV r ) was 9.4%, and the recovery was 111.7 ± 17.7%. γ -oryzanol is more stable in isopropanol than in hexane, in which 100% concentration could be maintained after refrigeration for 72 days.Index terms: Antioxidant, spectrophotometer, stability, phytochemistry. RESUMOγ -orizanol, um fitoquímico, tem atividade antioxidante e benefícios potenciais para saúde. Esse estudo objetivou desenvolver um método para extração de alta quantidade de γ -orizanol de farelo de arroz e verificar sua estabilidade em diferentes solventes. Dentre os cinco métodos estudados, o que teve maior rendimento em γ -orizanol foi melhorado, usando um delineamento composto central rotacional (DCCR -2 4 , incluindo 8 experimentos no ponto axial e 4 no ponto central, totalizando 28 experimentos). As variáveis testadas foram temperatura, tempo, volume e proporção de hexano: isopropanol usados na extração. Elevado conteúdo de γ -orizanol (13,98 mg g -1 ) foi obtido quando as condições de extração foram 40° C, 40 min e 75 mL de hexano:isopropanol (1:3). Os limites de detecção e quantificação do método foram 0,9 µg g -1 e 31,0 µg g -1 , respectivamente. A precisão instrumental foi 0,004%, a repetibilidade (CV r ) 9,4% e a recuperação 111,7 ± 17,7%. γ -orizanol é mais estável em isopropanol do que em hexano, onde 100% da concentração foi mantida após refrigeração por 72 dias.Termos para indexação: Antioxidante, espectrofotômetro, estabilidade, fitoquímica.

Pre‐ and Postharvest Strategies to Minimize Mycotoxin Contamination in the Rice Food Chain

“…Generally speaking, grains show the highest susceptibility to F. graminearum between the flowering and early stages of the grain (Scotti, Vergoignam, Feron, & Durand, 2001); the fungal contamination may occur in any part of the plant, however, the grains are the most susceptible part (Usha, Patkar, Shetty, Kennedy, & Lacey, 1993). Damage in rice structure is not very frequent (Dors, Primel, Fagundes, Mariot, & Furlong, 2011;Heidtmann-Bemvenuti, Nora, & Badiale-Furlong, 2012) since the external grain portion, formed by lignocellulosic and protein substances, is related to the fungal resistance associated to physical barriers and enzymatic inhibition. The production of trichothecenes, in particular, deoxinivalenol (DON), is a stress consequence promoted by the resistant cultivars (Keller et al, 2013).…”

Comparative Studies between Conventional and Microwave Assisted Extraction for Rice Bran Oil

“…nol can be extracted and can be further used in food, cosmetics, and pharmaceuticals industries 11,12 . Gamma-oryzanol can be obtained from the soap-stock of oil refinery process 11 .…”

Comparative study on conventional, ultrasonication and microwave assisted extraction of γ-oryzanol from rice bran

“…Numerous reports are available for the separation of nutraceuticals like γ-oryzanol from rice bran using traditional solvent extraction method (Heidtmann-Bemvenuti et al 2012;Oliveira et al 2012;Pascual et al 2013;Bhatnagar et al 2014;Ghasemzadeh et al 2015;Wang et al 2015). This method lacks efficiency in terms of time and solvent consumption.…”