2009
DOI: 10.1590/s1413-70542009000700041
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Abstract: , Edelclaiton Daros RESUMOO raquitismo-da-soqueira, causado por Leifsonia xyli subsp. xyli é considerado uma das mais importantes doenças bacterianas da cana-de-açúcar (Saccharum spp.). Para verificar a possível ocorrência de Leifsonia xyli subsp. xyli na região noroeste do Paraná, foi realizado um levantamento em 32 áreas de plantio de duas usinas, utilizando-se o método sorológico "Dot blot" e um protocolo baseado na reação em cadeia da polimerase. As amostras foram coletadas em áreas cultivadas com 8 varied… Show more

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“…Few studies have been conducted regarding PCR feasibility directly from vascular fluid over the years Comstock and Irey [4] Lopes et al [15] Wang et al [7]. Results from this work shows that fast methods of DNA extraction, which took approximately 2 hours for a total of 100 samples, may be as effective as more time-demanding and elaborated protocols that require around 24 hours to complete [16][17][18][19][20]. Therefore, large scale diagnosis of leaf scald in sugarcane may be performed through the DNA extraction techniques M2, M4 or M5, since these methods are fast, simple and do not use phenol as one of its reagents [21][22][23].…”
Section: Resultsmentioning
confidence: 99%
“…Few studies have been conducted regarding PCR feasibility directly from vascular fluid over the years Comstock and Irey [4] Lopes et al [15] Wang et al [7]. Results from this work shows that fast methods of DNA extraction, which took approximately 2 hours for a total of 100 samples, may be as effective as more time-demanding and elaborated protocols that require around 24 hours to complete [16][17][18][19][20]. Therefore, large scale diagnosis of leaf scald in sugarcane may be performed through the DNA extraction techniques M2, M4 or M5, since these methods are fast, simple and do not use phenol as one of its reagents [21][22][23].…”
Section: Resultsmentioning
confidence: 99%