Ação do BAP na regeneração in vitro de Blc Owen Holmes Ponkan x Brassavola digbiana nº 2

Giatti, Luciana; Lima, Giuseppina Pace Pereira

doi:10.1590/s1413-70542007000500001

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…Among the PGRs used in micropropagation, BA is the most frequently used cytokinin for plant differentiation and regeneration (Giatti et al 2007;Van Staden et al 2008;Phillips and Garda 2019). It plays an important role in shoot multiplication.…”

Section: Discussionmentioning

confidence: 99%

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

Beleski

Vendrame

2022

In Vitro Cell.Dev.Biol.-Plant

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Brassavola nodosa (L.) Lindl. is an epiphytic orchid with great potential for the ornamental plant industry. The lack of information on propagation and production techniques limits the development of large-scale commercial production. Furthermore, this species is experiencing a reduction in population due to habitat destruction, the impact of climate change, and over-collection from native habitats. This study aimed at developing an efficient protocol for micropropagation of B. nodosa, which could be valuable towards the large-scale commercial production as well as for conservation of this species. Six different concentrations of plant growth regulators (BA or IBA, with or without adenine sulfate) were evaluated in modified Murashige and Skoog (MS) medium for shoot multiplication. In addition, two concentrations of either NAA or IBA were evaluated for rooting. Explants were cultured under three different culture media conditions: semi-solid medium, liquid medium (partial immersion), and liquid medium (complete immersion). Results indicate that B. nodosa could be successfully micropropagated in liquid culture with partial immersion. The modified MS medium supplemented with 2.0 mg L−1 BA and 30.0 mg L−1 adenine sulfate resulted in higher multiplication rates. Rooting was obtained using either 0.5 mg L−1 NAA or 1.0 mg L−1 IBA with no significant differences between both rooting treatments. Plantlets achieved 100% ex vitro survival after 30-d acclimatization.

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Section: Discussionmentioning

confidence: 99%

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

Beleski

Vendrame

2022

In Vitro Cell.Dev.Biol.-Plant

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“…Some enzymes oxidize the phenols forming quinones, responsible for the brown color of the cultures, in addition to causing inhibition of the growth and death of explants. It is a species--dependent process and no method is fully effective for all cultivated species (GIATTI; LIMA, 2007).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Multiplication of Cassava Varieties

Menegazzo¹,

Rickli

Menegazzo

et al. 2019

ArqVet

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The implantation of a model of sustainable development for agriculture can happen with the contribution of the mandiocultura. But for this, culture needs to be strengthened. In vitro propagation is an instrument for this purpose. Micropropagation can provide growers with large quantities of vigorous and healthy cassava seedlings in a short time. The objective of this study was to evaluate the in vitro establishment of four varieties of cassava cultivated in the municipality of Colorado do Oeste, State of Rondônia, popularly known as Arara, Caturra, Cacau Vermelha and Roxinha. For that, an experiment was carried out in the Laboratory of In Vitro Cultivation at the Pole of Technological Innovation of the University of Cruz Alta (UNICRUZ), with a completely randomized design in a 4 x 2 factorial scheme, with 6 replications. The treatments consisted of explants grown in Murashige and Skoog (MS) medium without the presence of growth regulator and MS medium supplemented with of 6-benzylaminopurine (BAP). The results indicate that the average contamination percentage of the explants was 47.19%, differing among the varieties. The best growth response in culture media, in the multiple comparison of means (Scott-Knott's test, 5%), was obtained with MS medium without BAP addition, with significant difference between varieties. Under the conditions of this experiment, it was evidenced that micropropagation is a viable tool for obtaining varieties of interest, with desired phytosanitary qualities, with varietal and large scale authenticity.

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Ação do BAP na regeneração in vitro de Blc Owen Holmes Ponkan x Brassavola digbiana nº 2

Cited by 2 publications

References 4 publications

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

In Vitro Multiplication of Cassava Varieties

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