2003
DOI: 10.1590/s1413-70542003000300007
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Abstract: Realizou-se este trabalho com o objetivo de estudar condições nutricionais e hormonais para maximizar a produção de calos friáveis de carqueja [Baccharis trimera (Less.) DC]. Foi verificado que a iniciação de calo é dependente de fitorreguladores e da concentração do meio. A melhor indução de calo ocorreu em meio MS contendo 50% da concentração de sais, inositol e vitaminas, suplementado com 15,0 mM ANA. Proliferação de brotos foi obtida pelo uso de TDZ.

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Cited by 5 publications
(3 citation statements)
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“…According to Barrueto Cid (2000), different ways of balancing growth regulators change the morphogenic routes of plants grown in vitro; consequently, changes in phytoregulator concentrations play a key role at different micropropagation stages. Based on Silva et al (2003), callus induction is caused by tissue competence and/or chemical induction, and genotypes can respond to both phytoregulator classes -cytokinin and auxin -and to their different combinations.…”
Section: Resultsmentioning
confidence: 99%
“…According to Barrueto Cid (2000), different ways of balancing growth regulators change the morphogenic routes of plants grown in vitro; consequently, changes in phytoregulator concentrations play a key role at different micropropagation stages. Based on Silva et al (2003), callus induction is caused by tissue competence and/or chemical induction, and genotypes can respond to both phytoregulator classes -cytokinin and auxin -and to their different combinations.…”
Section: Resultsmentioning
confidence: 99%
“…Algumas delas atuam também no metabolismo do RNA, induzindo a transcrição de RNAs mensageiros que decodificam proteínas para o crescimento, podendo induzir uma desordenada proliferação celular, provocando o aparecimento de calos (GEORGE, 1996 O uso de auxinas é recomendado em vários estudos para a produção de calos friáveis. Silva et al (2003) estudando o efeito de reguladores de crescimento em carqueja detectaram maior indução e crescimento de calos friáveis na presença de ANA. Pereira et al (2007) também observaram a necessidade do uso de auxina para a indução de calos friáveis em explantes foliares de Uncaria guianensis J. F. Gmel.…”
Section: Anaunclassified
“…The influence of various growth regulators and medium concentrations, in different quantities, on the in vitro callus induction of B. trimera was evaluated. 65 It was found that the callus initiation was dependent on both the growth regulator and medium concentration. The highest callus induction and development were obtained by using 15 µM of 1-naphthaleneacetic acid as growth regulator and half strength of salts, vitamins and myo-inositol of Murashige and Skoog medium.…”
Section: Baccharis Plant Cell Culturesmentioning
confidence: 99%