3º Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 (FAN): recomendações para padronização do ensaio de pesquisa de autoanticorpos em células HEp-2, controle de qualidade e associações clínicas

Dellavance, Alessandra; Júnior, Alexandre Gabriel; Nuccitelli, Barbara; Taliberti, Ben Hur; Mühlen, Carlos Alberto von; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Bueno, Cleonice; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Cardoso, E.C.; Bonfá, Eloísa; Araújo, Flávia Ikeda e; Rassi, Gustavo Gabriel; Mundim, Hugo Mendonça; Bendet, Izidro; Rêgo, Jozélia; Vieira, Lisiane Maria Enriconi dos Anjos; Andrade, Luiz Eduardo Coelho; Barbosa, Maria Ordália Ferro; Sugiyama, Mitiko; Santiago, Mittermayer Barreto; Slhessarenko, Natasha; Silva, Nílzio Antônio da; Francescantônio, Paulo Luiz Carvalho; Jarach, Renata; Suda, Roberto; Levy, Roger A.; Sampaio, Silvia Oliveira; Neves, Suzane Pretti Figueiredo; Cruvinel, Wilson de Melo; Santos, Wilton Silva dos; Nóbrega, Yanna Karla de Medeiros

doi:10.1590/s0482-50042009000200002

Cited by 29 publications

(16 citation statements)

References 8 publications

(13 reference statements)

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“…In 2007, the III BCA HEp-2 focused efforts on encouraging Brazilian laboratories to adopt quality control programs for the HEp-2 IIFA test, as well as educational programs. The relevance of conjugate titration was highlighted as a crucial step to adjust the reading system and harmonize the titer of results among different laboratories [3,4].…”

Section: Introductionmentioning

confidence: 99%

V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells

et al. 2019

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Background: The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses.Mainbody: Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized.(Continued on next page)

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Section: Introductionmentioning

confidence: 99%

V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells

et al. 2019

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“…The diversity of commercial kits in the international and nationals markets has given rise to this study. Moreover, uncontrolled experiments at laboratories specialized in autoimmunity have substantiated HEp-2 (2) , followed by groups from Germany (16) , France (15) , Denmark (21) and Argentina (1) .…”

Section: Discussionmentioning

confidence: 99%

“…A panel of human serum samples ( Table 1) was selected from the autoantibody serum bank at Fleury Medicina e Saúde -Research and Development Department (R&D) to represent the most relevant IIF patterns on HEp-2 and also acknowledged by the Brazilian Consensus for autoantibodies on HEp-2 (2) . The samples were sequentially numbered so that it was not possible to identify its immunological specificity ( Table 2).…”

Section: Serum Samplesmentioning

confidence: 99%

Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

Dellavance

Cruvinel²,

Francescantônio³

et al. 2013

J. Bras. Patol. Med. Lab.

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Introduction: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the recognition of indirect immunofluorescence patterns associated with several autoantibodies. Objective: To evaluate a panel of serum samples representing nuclear, nucleolar, cytoplasmic, mitotic apparatus, and chromosome plate patterns on HEp-2 cell substrates from different suppliers. Materials and methods: Seven blinded observers, independent from the three selected reference centers, evaluated 17 samples yielding different nuclear, nucleolar, cytoplasmic and mitotic apparatus patterns on HEp-2 cell slides from eight different brands. The slides were coded to maintain confidentiality of both brands and participating centers. Results: The 17 HEp-2 cell patterns were identified on most substrates. Nonetheless, some slides showed deficit in the expression of several patterns: nuclear coarse speckled/U1-ribonucleoprotein associated with antibodies against RNP (U1RNP), centromeric protein F (CENP-F), proliferating cell nuclear antigen (PCNA), cytoplasmic fine speckled associated with anti-Jo-1 antibodies (histidyl synthetase), nuclear mitotic apparatus protein 1 (NuMA-1) and nuclear mitotic apparatus protein 2 (NuMA-2). Conclusion: Despite the overall good quality of the assessed HEp-2 substrates, there was considerable inconsistency in results among different commercial substrates. The variations may be due to the evaluated batches, hence generalizations cannot be made as to the respective brands. It is recommended that each new batch or new brand be tested with a panel of reference sera representing the various patterns.

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“…The estimated population of Cascavel for 2008 by the Brazilian Institute of Geography and Statistics (IBGE) was 291,747 inhabitants (149,790 female and 141,957 male), and these fi gures were used for calculating the incidence. 7 Data were shown as frequencies, medians, and means ± standard deviation, with a 95% confi dence interval.…”

Section: Methodsmentioning

confidence: 99%

Incidência e aspectos clínico-laboratoriais do Lúpus eritematoso sistêmico em cidade do Sul do Brasil

Nakashima

Galhardo

Silva

et al. 2011

Rev. Bras. Reumatol.

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Introduction: Brazilian epidemiological studies on systemic lupus erythematosus (SLE) are scarce, and currently available data originate almost entirely from international literature. Objectives: To determine the incidence and some clinical and laboratory characteristics of patients with SLE in the municipality of Cascavel, state of Paraná, Brazil. Patients and Methods: Data were collected from August 2007 to July 2008 in all health services of Cascavel providing health care in rheumatology: a university-affi liated hospital, a public outpatient clinic, and three private clinics. Results: The study identifi ed 14 patients diagnosed with SLE, which resulted in an estimated incidence of 4.8 cases/100,000 inhabitants/ year. All patients were female, and the mean age was 41.5 years. The highest incidence of disease occurred between 30 and 39 years of age, and 92.8% of patients met at least four of the 11 American College of Rheumatology (ACR) criteria for diagnosis of SLE. The drug treatment of patients was also assessed and proved to be in accordance with the Brazilian Consensus for Treatment of SLE. Conclusion: The incidence obtained in the municipality of Cascavel is close to those reported in international studies.

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3º Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 (FAN): recomendações para padronização do ensaio de pesquisa de autoanticorpos em células HEp-2, controle de qualidade e associações clínicas

Cited by 29 publications

References 8 publications

V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells

V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells

Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

Incidência e aspectos clínico-laboratoriais do Lúpus eritematoso sistêmico em cidade do Sul do Brasil

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