Retinite por citomegalovirus (CMV) após terapia imunossupressora para vasculite leucocitoclástica

Rodrigues, Carlos Ewerton Maia; Gadelha, Cláudia Sarmento; Nobre, Christiane Aguiar; Callado, Maria Roseli Monteiro; Vieira, Rejane Maria Rodrigues de Abreu; Vieira, Walber Pinto

doi:10.1590/s0482-50042009000100007

Cited by 1 publication

(1 citation statement)

References 17 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This persistent nature of HCMV infection facilitates the use of molecular and immunological diagnostic tools. It also allows the molecular and epidemiological profiling of virus isolates from individuals with risk factors for infection, such as immunosuppression and living in an underdeveloped country where the HCMV seroprevalence is elevated [9][10][11][12][13] . Molecular analyses by restriction fragment length polymorphism (RFLP) and sequencing of the glycoprotein B (gB)-encoding region of the HCMV viral envelop are used as basic tools for genotypic classification of the virus and the association between genotypes and immunodeficient patient categories as assessed by clinicians [14][15][16][17][18][19][20][21][22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil

Silva

Medeiros

Moraes

et al. 2011

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction:Human cytomegalovirus is an opportunistic betaherpesvirus that causes persistent and serious infections in immunodeficient patients. Recurrent infections occur due to the presence of the virus in a latent state in some cell types. It is possible to examine the virus using molecular methods to aid in the immunological diagnosis and to generate a molecular viral profile in immunodeficient patients. The objective of this study was to characterize cytomegalovirus genotypes and to generate the epidemiological and molecular viral profile in immunodeficient patients. Methods: A total of 105 samples were collected from immunodeficient patients from the City of Belém, including newborns, hemodialysis patients, transplant recipients and HIV+ patients. An IgG and IgM antibody study was completed using ELISA, and enzymatic analysis by restriction fragment length polymorphism (RFLP) was performed to characterize viral genotypes. Results: It was observed that 100% of the patients had IgG antibodies, 87% of which were IgG+/IgM-, consistent with a prior infection profile, 13% were IgG+/IgM+, suggestive of recent infection. The newborn group had the highest frequency (27%) of the IgG+/IgM+ profile. By RFLP analysis, only one genotype was observed, gB2, which corresponded to the standard AD169 strain. Conclusions: The presence of IgM antibodies in newborns indicates that HCMV continues to be an important cause of congenital infection. The low observed genotypic diversity could be attributed to the small sample size because newborns were excluded from the RFLP analysis. This study will be continued including samples from newborns to extend the knowledge of the general and molecular epidemiology of HCMV in immunodeficient patients.

show abstract