2009
DOI: 10.1590/s0365-05962009000300006
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Abstract: FUNDAMENTOS - A alta frequência das micoses cutâneas justifica a necessidade de avaliar a possível contribuição da determinação do perfil de susceptibilidade aos antifúngicos in vitro. OBJETIVO - Avaliar se existe variabilidade nos isolados fúngicos quanto à susceptibilidade in vitro de fungos filamentosos, previamente isolados de micoses cutâneas, frente aos antifúngicos fluconazol, cetoconazol, itraconazol e terbinafina. MÉTODOS - Os fungos foram isolados e identificados por meio da metodologia clássica e o … Show more

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Cited by 11 publications
(12 citation statements)
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“…Minimum inhibitory concentration (MIC) was determined according to the National Committee for the Clinical Laboratory Standards Institute (CLSI) guidelines for fi lamentous fungi (26) , with adaptations described in a previous study (27) . Roswell Park Memorial Institute medium (RPMI-1640; SigmaAldrich Inc., St. Louis, MO, USA) containing L-glutamine, lacking sodium bicarbonate, and buffered with 0.165mol·L…”
Section: Antifungal Screening and Susceptibility Testingmentioning
confidence: 99%
See 1 more Smart Citation
“…Minimum inhibitory concentration (MIC) was determined according to the National Committee for the Clinical Laboratory Standards Institute (CLSI) guidelines for fi lamentous fungi (26) , with adaptations described in a previous study (27) . Roswell Park Memorial Institute medium (RPMI-1640; SigmaAldrich Inc., St. Louis, MO, USA) containing L-glutamine, lacking sodium bicarbonate, and buffered with 0.165mol·L…”
Section: Antifungal Screening and Susceptibility Testingmentioning
confidence: 99%
“…The essential oil from E. smithii was solubilized in the culture medium and was serially diluted to achieve eight concentrations ranging from 7.8 to 1,000μg·mL , respectively, were used as reference drugs. These antifungal agents were chosen because they have standard testing protocols and because they are the most widely used oral antifungal drugs in clinical practice (18) (27) (28) . The microdilution plates were incubated at 28°C and were examined after seven days of incubation.…”
Section: Antifungal Screening and Susceptibility Testingmentioning
confidence: 99%
“…1,2 When compared to other skin fungal infections, it is the most frequent and the most difficult to treat. 3,4 …”
Section: Introductionmentioning
confidence: 99%
“…1 Evaluation of in vitro efficacy is done by reference method, broth dilution or Agar diffusion. 7,8 In Brazil, Almeida et al evaluated the susceptibility of fungi that cause superficial mycoses to antifungal agents through the microdilution technique. 8 Nevertheless, there is no reference methodology for terbinafine and more studies are necessary for its validation.…”
Section: Introductionmentioning
confidence: 99%
“…7,8 In Brazil, Almeida et al evaluated the susceptibility of fungi that cause superficial mycoses to antifungal agents through the microdilution technique. 8 Nevertheless, there is no reference methodology for terbinafine and more studies are necessary for its validation. In addition, there is no technique that is easily employed in clinical laboratories to search for the ideal therapy in cases of mycoses caused by filamentous fungi, including dermatophytes, such as those that exist in cases of yeast infections.…”
Section: Introductionmentioning
confidence: 99%