Identificação e caracterização de isolado brasileiro do vírus de poliedrose nuclear da lagarta do cartucho-do-milho

Abstract

An. Soc. Entomol. Brasil 26(3): 507-515 (1997) Identification and Characterization of a Brazilian Isolate of Fall Armyworm Nuclear Polyhedrosis Virus ABSTRACT -A nuclear polyhedrosis virus (NPV) from Spodoptera frugiperda (Smith) caterpillars (Lepidoptera: Noctuidae), collected from diseased insects in Sete Lagoas, MG, was confirmed and identified. The pathology of infected caterpillars is characteristic of NPV. The virus showed tropism by ectoderm cells, fat cells, and tracheocytes. Viral particles (polyhedra and alkali released virions -ARVs) were purified through diferential centrifugations in sucrose gradient, and the polyhedral band was located in the inferior third part of the tube. Increasing time of polyhedral digestion with alkaline solution resulted in higher dissolution of occluded bodies (polyhedra) consequently releasing virions. Five bands were formed for the alkali released virions. The electrophoretic pattern of S. frugiperda NPV was compared to the one of Anticarsia gemmatalis (Hübner) NPV for polyhedra as well as ARVs. A peptide of 32,000 dalton was characterized as the polyhedrin of S. frugiperda NPV.

Estudos de Bacillus thuringiensis Berliner visando ao controle de Spodoptera frugiperda (J.E.Smith).

“…No Brasil, a ocorrência de um vírus de poliedrose nuclear (VPNSf) em S. frugiperda foi constatada em 1978 (Gerk et al, 1997), sendo que Cruz et al (1997), Moscardi (1998), Valicente & Costa (1995), Valicente & Cruz (1991) Lecuona (1999) e Lezama et al (1996) relataram a alta virulência deste fungo para a lagarta-do-cartucho na Guatemala, México e Argentina, respectivamente.…”

Molecular biology of baculovirus and its use in biological control in Brazil

“…A partir de uma lagarta-do-campo, um nucleopoliedrovírus foi identificado como SfNPV e purificado (Valicente et al, 1989). Em prosseguimento, metodologias para o controle biológico da lagarta-do-cartucho pelo SfNPV foram estabelecidas (Valicente & Cruz, 1991), e estudos de caracterização biológica e bioquímica foram conduzidos (Gerk et al, 1997). Até o momento, aproximadamente 5.000 hectares de milho foram tratados com esse vírus, o qual é produzido em lagartas criadas em dieta artificial e formulado como pó molhável, para distribuição aos agricultores.…”