TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES

Abstract

Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed, determining protein and protein plus carbohydrate, respectively. ELISA was performed from sediments. The results showed that 51 (57.3%) of the isolates were toxigenic to mice when inoculated by intraperitoneal route; bacteria from different organs had variable patterns of toxigenicity. Toxigenicity of EPS extracts was only expressed when protein concentration was 0.04 mg/mL and between 0.31 and 0.5 mg/mL for carbohydrate. Isolates were characterized as toxigenic when showing optimum protein and carbohydrate concentrations

Genotyping of Clostridium perfringens associated with sudden death in cattle

“…In the present trial, most strains (82.6%) were found to be C. perfringens type A, similarly to a recent report by Manteca et al (15). In Brazil, Baldassi et al (16) reported the typing of 89 C.…”

Influence of Growth Stages and Nutrient Media on Production of Membrane Vesicles of Clostridium perfringens Type A and their Molecular Characterization

“…All the five isolates were reconfirmed by inoculating onto 5 percent (v/v) sheep blood agar to observe the typical smooth, round and glistening colonies surrounded by inner zone of complete haemolysis and outer zone of partial haemolysis as described by Baldassi et al, (2002). All experiments were performed anaerobically in the anaerobic jar with anaerogas pack system (Hi-Media, Mumbai, India) at 37C for 24 hrs.…”

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India

“…The production of phospholipase C by the C. perfringens isolates was verified according to the literature (5). Suspected colonies were inoculated into Wills and Hobb's medium without lecithinase-specific antisera, and added to neomycin sulfate at the concentration of 250 mg per mL.…”