The use of snake venom derived fibrin glue in hysterorrhaphy of ovine caesarean surgery

Abstract

Fibrin glue has been used on its own or in conjunction with suturing materials to promote hemostasis, reduce adherence, strengthen the wound site, and improve healing. Snake venom derived fibrin glue was evaluated as an alternative to conventional uterine suturing after ovine caesarean surgery. Twenty-eight pregnant ewes of known mating date were used. The animals submitted to conventional caesarean sections showed a better wound healing process. As expected, all the operated animals had retained placenta, compromising coaptation of wound edges. This had a strong influence in the results observed with the animals in which fibrin glue was used. The animals were divided into four groups GI, GII, GIII, and GIV and sacrificed, respectively, 3, 7, 15, and 30 days after surgery for macro and microscopic examination of the uterus. From each group, six animals underwent surgery using fibrin glue and four animals were submitted to comparative conventional hysterorrhaphy using catgut

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…G2: macroscopic alterations were more intense. There was no difference in the values of force of rupture between the two groups.Fibrin sealant derived from snake venom proved efficient on allowing anastomosis of the colon of rats.The use of fibrin sealant in the anastomosis requires further investigation in larger animals to understand the sealant behavior on larger organs. Chalhoub et al (2000) [42] To evaluate fibrin sealant derived from snake venom as an alternative to conventional uterine suture after ovine cesarean surgery.Twenty-eight pregnant sheep divided into four groups of seven animals: six animals were submitted to surgery with fibrin sealant and one animal underwent the conventional hysterorrhaphy.The healing of the wound showed good macroscopic appearance 30 days after surgery with the application of the sealant. However, microscopically, the uterus did not return to pre-pregnancy conditions 30 days after the experimental cesarean section.…”

“…Chalhoub et al [42] performed hysterorrhaphy after cesarean sections of sheep. The mortality rate of the animals was 12.5% , which was considered high.…”

Snake venom derived fibrin adhesive in dog uterine healing: biomechanical study

“…Most surgical wounds require constant and efficient mechanical support to keep the wound edges together during healing; suture is the preferred method (25). Fibrin glue has been used in place of or as reinforcement for suture to promote hemostasis, reduce adherence, strengthen the wound site, and improve healing (6).…”

“…The animals submitted to conventional caesarean sections presented a better wound healing. All operated animals had retained placenta, and compromised wound edge coaptation, mainly in fibrin glue animals (6). The advantages of fibrin glue include tissue compatibility, biodegradability, and efficacy when applied to humid or highly vascularized surfaces (5); therefore further studies on its use in past caesarean hysterorrhaphy in different animal species are needed (6).…”

Reparo ósseo de enxerto autógeno estabilizado com adesivo de fibrina: análise histológica e histomorfométrica

“…A eficácia deste novo selante de fibrina é relatada na literatura, havendo de resultados satisfatórios quando utilizado em animais visando reparos nervosos (IUAN et al, 1995;FRANCIOSI;PALHARES, 1995;CHALHOUB et al 2000;SILVA et al, 2012;VITERBO et al, 2012) e reparos teciduais (RAHAL et al, 2004;FERRARO et al, 2005;SAMPAIO et al, 2007). BARANAUSKAS; da CRUZ-HOFLING et al, 2004;KAZEM SHAKOURI et al, 2010).…”