An evaluation by rat colon anastomosis of the efficacy of fibrin glue derived from snake venom

Abstract

The objective of this study was to evaluate the efficacy of fibrin adhesive made up of snake venom and bubaline fibrinogen by rat colon anastomosis. Eighty rats were randomly assigned into 2 experimental groups: GI control (anastomosis with extramucous interrupted suture) and GII (repair suture + fibrin glue). The animals were studied at the following 4 times: T0 – preoperative, T1 – 7th day postoperative, T2 – 14th day postoperative, and T3 – 21 st day postoperative. The macroscopic characteristics of the intestinal segment open and closed anastomosis and the bursting strength of the anastomosed segments were observed at each of the above times. The results showed that the anastomosed segments coapted and there was no difference in the bursting strength values between the 2 groups. There was a decrease in the bursting strength values up until the 7 th day postoperative in both groups with its progressive increase at the other times. Although important experimental studies using large animals are needed for a better evaluation of tissue repair processes, this adhesive may become a valuable tool for use in anastomosis

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

“…Factor XIII, when activated by thrombin in the presence of calcium (Ca 2+ ), reacts with the fibrin polymers, forming a stable and insoluble clot (Martinowitz and Spotnitz, 1997). These glues are used for tissue regeneration procedures in different areas of medicine as hemostatic agents, healing inducers, cavity sealants, and drug-delivery vehicles (Borst et al, 1982;Kram et al, 1989;Alving et al, 1995;Carvalho et al, 1997;Sartori Filho et al, 1998;Leite et al, 2000;Rahal et al, 2004;Dal Pizzol et al, 2009). However, the role of fibrin sealants in the healing of bone fractures and osteogenesis is not fully understood, with some studies recommending its application, whereas others do not (Abiraman et al, 2002).…”

Use of a New Fibrin Sealant and Laser Irradiation in the Repair of Skull Defects in Rats

“…Thus, a new fibrin sealant derived from an enzyme obtained from snake venom and fibrinogen extracted from large animals was developed for tissue regeneration. This new fibrin sealant acts as a substrate for cell growth and shows total biocompatibility, as demonstrated by researches in animals and humans (8)(9)(10)(11)(12). Therefore, the objective of the present study was to evaluate the osteogenic and healing capacity of this new fibrin sealant in combination with autogenous bone grafts and laser irradiation during the healing of experimentally induced skull defects in rats.…”

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

“…In addition, patients may develop antibodies against bovine thrombin 13 . In an attempt to overcome these difficulties, another sealant consisting of a thrombin-like enzyme extracted from snake venom and fibrinogen obtained from large animals to replace human components has shown excellent results in experimental studies on the regeneration of different tissues [14][15][16][17][18] , in mesenchymal cell cultures 19 , and in the healing of skin wound, venous ulcers and gingival graft sutures in humans 20,21 . This new sealant is a biodegradable product that does not cause adverse reactions, does not contain human blood components, has good adhesive capacity, and does not transmit infectious diseases 22 .…”