Physico-chemical, spectroscopical and thermal characterization of biodiesel obtained by enzymatic route as a tool to select the most efficient immobilized lipase

Silva, G. A. M.; Rós, Patrícia Caroline Molgero Da; Souza, Leandro Torres de; Costa, Ana Paula Oliveira; Castro, Heizir F. de

doi:10.1590/s0104-66322012000100005

Cited by 24 publications

(12 citation statements)

References 31 publications

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“…The calibration curves were generated with standard ethyl esters using hexanol as the internal standard. The reaction yield was calculated by taking into account the mass of ester content obtained by GC analysis and the total theoretical ester mass based on the initial amount of both oil and ethanol (Urioste et al, 2008;Silva et al, 2012).…”

Section: Monitoring Ethyl Ester Formationmentioning

confidence: 99%

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Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

Tiosso¹,

Carvalho

Castro

et al. 2014

Braz. J. Chem. Eng.

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-This work reports the use of commercially available immobilized lipase preparations (Novozym® 435 and Lipozyme TL IM, both from Novozymes, and Lipase PS IM from Amano) as catalysts in the transesterification reaction of different alkyl-chain triglycerides with ethanol. The ethanolysis of native oils from Brazilian Amazon plants andiroba (Carapa guianensis), babassu (Orbignya sp.), jatropa (Jatropha curcas), and palm (Elaeis sp.) was studied in a solvent-free system. In a typical reaction, the immobilized preparations were added to the mixture of vegetable oil-to-ethanol in a molar ratio of 1:9. The reactions were performed at 50 °C for a maximum period of 48 h. Under the conditions used, all the immobilized lipase preparations were able to generate the main esters of fatty acids present in the tested feedstocks, and both the reaction rate and ester yield were dependent on the source of lipase and vegetable oil. The viscosity values for the samples obtained in each reaction displayed a consistent reduction in relation to their original feedstocks, which also confirms the high conversion of triglycerides to ethyl esters (99.8-74.0%). The best performances were obtained with Amano PS IM and Novozym® 435, with the biodiesel samples from the babassu and jatropha oils exhibiting viscosity values in accordance with those predicted by the technical standards of ASTM D6751 (1.9-6.0 mm 2 /s). Lipozyme TL IM displayed an unsatisfactory performance, indicating that the conditions of the transesterification reaction should be improved. This comparative study using different catalysts and several vegetable oil sources with varying fatty acid compositions is particularly important for all tropical countries with a diversity of native vegetable oil sources.

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Section: Monitoring Ethyl Ester Formationmentioning

confidence: 99%

“…The temperature was maintained at 20 °C during the assays. Biodiesel samples of 2.0 mL were used, and the measurements replicated three times (Silva et al, 2012).…”

Section: Viscosity and Density Determinations Of Purified Fatty Acid mentioning

confidence: 99%

Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

Tiosso¹,

Carvalho

Castro

et al. 2014

Braz. J. Chem. Eng.

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“…Covalent attachment of nucleophilic groups of lipases (N-terminal and hydroxyl groups) on highly activated supports (e.g., epoxy or aldehyde groups on the support surface introduced after the activation step) may promote the stabilization of their three-dimensional structures, thus reducing possible conformational changes induced by any distorting agents such as solvents and temperature [18, 21–24]. Previous studies carried out in our lab have reported the preparation of robust biocatalysts prepared by covalent attachment of several lipases on activated hybrid support SiO 2 -PVA for further application in biotransformation reactions [21, 22, 25]. …”

Section: Introductionmentioning

confidence: 99%

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Souza

Mendes

Castro

2016

BioMed Research International

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The present study deals with the enzymatic synthesis of biodiesel by transesterification of Jatropha oil (Jatropha curcas L.) with ethanol in a solvent-free system. Seven commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-polyvinyl alcohol composite (epoxy-SiO2-PVA) were tested as biocatalysts. Among them, immobilized lipases from Pseudomonas fluorescens (lipase AK) and Burkholderia cepacia (lipase PS) were the most active biocatalysts in biodiesel synthesis, reaching ethyl ester yields (FAEE) of 91.1 and 98.3% at 72 h of reaction, respectively. The latter biocatalyst exhibited similar performance compared to Novozym® 435. Purified biodiesel was characterized by different techniques. Transesterification reaction carried out under microwave irradiation exhibited higher yield and productivity than conventional heating. The operational stability of immobilized lipase PS was determined in repeated batch runs under conventional and microwave heating systems, revealing half-life times of 430.4 h and 23.5 h, respectively.

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“…The most suitable conditions were FAD to methanol molar ratio of 1:6 and a reaction temperature of 300 °C during 30 min, in which 95% of FAMEs was produced. Compared to the conventional acid-catalyzed esterification of FAD using a similar molar ratio, they found that only a 75% yield was obtained in 5 h. Silva et al (2012) obtained biodiesel from beef tallow with ethanol, reaching reaction times between 24-48 hours at 45 °C. The authors used an enzymatic route with two different lipases.…”

Section: Heterogeneous and Enzymatic Catalystsmentioning

confidence: 99%

Conversion of by-products from the vegetable oil industry into biodiesel and its use in internal combustion engines: a review

Piloto‐Rodríguez¹,

Melo²,

Pérez³

et al. 2014

Braz. J. Chem. Eng.

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-Biodiesel produced from by-products and waste materials can be an economical way of reducing traditional oil consumption and environmental problems. The by-products from the vegetable oil refining industry such as soapstock, acid oil and fatty acid distillates are suitable for producing biodiesel. The present work is a survey related to the use of these by-products to obtain biodiesel, covering not only the traditional and most widely used acid/base catalysis, but also solid and enzymatic catalysis. Details of the techniques are presented and compared. The advantages and drawbacks of the different approaches are mentioned and analyzed. The synthesis and use of by-products from the vegetable oil refining industry are covered in this work. The use of the obtained biodiesel in diesel engines is also included, demonstrating the disparity between the number of papers related to biodiesel production and engine performance assessment.

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Physico-chemical, spectroscopical and thermal characterization of biodiesel obtained by enzymatic route as a tool to select the most efficient immobilized lipase

Cited by 24 publications

References 31 publications

Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Conversion of by-products from the vegetable oil industry into biodiesel and its use in internal combustion engines: a review

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