2001
DOI: 10.1590/s0104-66322001000100009
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Abstract: Protozoa are important microorganisms for the ecosystem balance in wastewater treatment plants. A procedure for their semi-automated identification and counting based on image analysis is proposed. The main difficulty is segmentation of the protozoa as most of them are in contact with the sludge. The protozoa are characterized by the size of their silhouette (area and length) and three shape factors (elongation, circularity and eccentricity). They are identified after projecting the resulting 5D space into a 3… Show more

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Cited by 18 publications
(13 citation statements)
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“…Crossed work Besides the related work above, the research of EM classification (Akiba and Kahui 2000;Amaral 2003;Amaral et al 1999Amaral et al , 2004Amaral et al , 2008Chen and Li 2008;Ferreira and Rasband 2012;Ginoris et al 2006Ginoris et al , 2007aGray et al 2002;Jenne et al 2001Jenne et al , 2002Jenne et al , 2003Kiranyaz et al 2011;Chen 2008, 2009;Motta et al 2001;Ruusuvuori et al 2008;Thiel and Davies 1995;Thiel andWiltshire 1995 in Sect. 3 andMM classification (Javidi et al 2006g) in Sect.…”
Section: Original Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Crossed work Besides the related work above, the research of EM classification (Akiba and Kahui 2000;Amaral 2003;Amaral et al 1999Amaral et al , 2004Amaral et al , 2008Chen and Li 2008;Ferreira and Rasband 2012;Ginoris et al 2006Ginoris et al , 2007aGray et al 2002;Jenne et al 2001Jenne et al , 2002Jenne et al , 2003Kiranyaz et al 2011;Chen 2008, 2009;Motta et al 2001;Ruusuvuori et al 2008;Thiel and Davies 1995;Thiel andWiltshire 1995 in Sect. 3 andMM classification (Javidi et al 2006g) in Sect.…”
Section: Original Methodsmentioning
confidence: 99%
“…In Amaral et al (1999), a semi-automatic multi-class classification method is proposed to identify seven protozoa species in wastwater treatment, where image denoising and enhancing are first applied, then five shape features are extracted, lastly PCA techniques are used to build a 3D discriminative model for the classification. As a development of the above work, Amaral (2003), Motta et al (2001) improve the functions of this system, where image segmentation methods are newly applied and six shape features are used. In the experimental phase, 14 protozoa species are tested, and an overall accuracy higher than 50% is obtained.…”
Section: Overview Of Em Classificationmentioning
confidence: 99%
“…Protozoa is also related in activation sludge in wastewater treatment besides bacteria. Protozoa also maintain good balance in the biological ecosystem by eliminating the excess bacteria, promote their growth and promote floc [28]. Protozoa can mineralize carbon in activated sludge and can eliminate mineral nutrient when the carbon source is expedited by bacteria.…”
Section: Protozoamentioning
confidence: 99%
“…The identification of protozoa and metazoa populations, which are among the most important indicators of activated sludge status (Madoni, 1994b), is another important field of application of image analysis. Coupled with multivariate statistical techniques, such as PCA (Amaral et al 1999;da Motta et al 2001a) and discriminant analysis (DA) (Amaral et al 2004a), morphological parameters can be used to recognize >80 % of the main 22 protozoa and metazoa species commonly present in aerated tanks. Ginoris et al (2007a, b) further corroborated the usefulness of DA and ANN for identification purposes as well as the possibility of real-time analysis (2-3 h), while avoiding the tedious task of traditional manual identification.…”
Section: Full-scale Wwt Plantsmentioning
confidence: 99%