The effects of trace elements, cations, and environmental conditions on protocatechuate 3,4-dioxygenase activity

Silva, Andréa Scaramal da; Jacques, Rodrigo Josemar Seminoti; Andreazza, Robson; Bento, Fátima Menezes; Camargo, Flávio Anastácio de Oliveira

doi:10.1590/s0103-90162013000200002

Cited by 10 publications

(5 citation statements)

References 20 publications

(7 reference statements)

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“…After ten cycles, the activity was kept around 60%; suggesting greater operational stability of the immobilized 3,4-POD. Although no reusability data of 3,4-POD was reported by the previous studies 19 20 21 22 , similar data can be comparable to other enzymes immobilized on different supports 48 51 .…”

Section: Resultssupporting

confidence: 51%

“…The covalent method is preferred to the non-covalent in order to develop a stable and robust MWCNT-3,4-POD hybrid system. The coupling buffer (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) (50 mM, pH 8.0) was used to maintain the MWCNT colloidal stability and immobilization efficiency 18 20 22 . Immobilized 3,4-POD with high activity was obtained only after 2 h of incubation as shown in Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The MA has not shown toxicity effects on normal cells 16 , and is an industrially valuable compound for adipic acid production (2.3 million metric tons/year and expected market value was 6.3 billion pounds in 2012) 17 . A few studies have been attempted to immobilize 3,4-POD onto porous glass 18 19 , agarose 20 21 and alginate matrix 21 22 . However, the studies were done without proper scrutiny.…”

mentioning

confidence: 99%

“…However, the studies were done without proper scrutiny. They have used the crude 3,4-POD extracts 21 22 , and uncharacterized immobilized 3,4-POD structures and apparent kinetic details. Hence, these data should be used with caution in understanding the 3,4-POD catalytic efficiency and behavior.…”

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confidence: 99%

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Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Das

Hamid

Annuar

2016

Sci Rep

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The present study reported for the first time covalent immobilization of protocatechuate 3,4-dioxygenase (3,4-POD) onto functionalized multi-walled carbon nanotubes (F-MWCNT) for degrading the toxic 3,4-dihydroxybenzoic acid (3,4-DHBA) pollutant in water. The F-MWCNTs had a maximum 3,4-POD loading of 1060 μg/mg. Immobilized 3,4 POD had 44% of relative structural changes to its free configurations. Nevertheless, >90% of relative activity and about 50% of catalytic efficiency were retained to the free enzyme. Immobilized 3,4-POD demonstrated higher alkaline stability and thermostability than the free 3,4-POD. The free and immobilized 3,4-POD lost 82% and 66% of relative activities, respectively after 180 min of incubations at 90 °C. Excellent shelf-life was observed for the immobilized 3,4-POD with residual activity of 56% compared with 41% and 39% of the free 3,4-POD at 4 °C and 25 °C over 30 days storage. Immobilized 3,4-POD showed >60% of catalytic activity retention even after ten-cycle uses, defraying the expenses of free 3,4-POD productions for long term uses. Finally, the immobilized 3,4-POD removed 71% of 3,4-DHBA from water in <4 h, paving its future application for water purification with reduced costs and time.

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Das

Hamid

Annuar

2016

Sci Rep

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“…The slight decrease of activity due to ascorbate, a reductant, is a property consistent with a prostetic Fe 3+ responsible for catalysis. Fe 2+ -containing enzymes are sensitive to oxidizing agents whereas Fe 3+ -containing enzymes are often activated by them [29].…”

Section: Resultsmentioning

confidence: 99%

Biotransformation of Eugenol via Protocatechuic Acid by Thermophilic Geobacillus sp. AY 946034 Strain

Giedraityte¹,

Kalėdienė²

2014

Journal of Microbiology and Biotechnology

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The metabolic pathway of eugenol degradation by thermophilic Geobacillus sp. AY 946034 strain was analyzed based on the lack of data about eugenol degradation by thermophiles. TLC, GC-MS, and biotransformation with resting cells showed that eugenol was oxidized through coniferyl alcohol, and ferulic and vanillic acids to protocatechuic acid before the aromatic ring was cleaved. The cell-free extract of Geobacillus sp. AY 946034 strain grown on eugenol showed a high activity of eugenol hydroxylase, feruloyl-CoA synthetase, vanillate-O-demethylase, and protocatechuate 3,4-dioxygenase. The key enzyme, protocatechuate 3,4- dioxygenase, which plays a crucial role in the degradation of various aromatic compounds, was purified 135-fold to homogeneity with a 34% overall recovery from Geobacillus sp. AY 946034. The relative molecular mass of the native enzyme was about 450 ± 10 kDa and was composed of the non-identical subunits. The pH and temperature optima for enzyme activity were 8 and 60°C, respectively. The half-life of protocatechuate 3,4-dioxygenase at the optimum temperature was 50 min.

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Introduction

Das¹

2017

Carbon Nanostructures

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The effects of trace elements, cations, and environmental conditions on protocatechuate 3,4-dioxygenase activity

Cited by 10 publications

References 20 publications

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Biotransformation of Eugenol via Protocatechuic Acid by Thermophilic Geobacillus sp. AY 946034 Strain

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