Assessment of inter- and intra-cultivar variations in olive using SSR markers

Abstract

Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this high level of genetic variation is probably due to the location of However, there was some confusion about the identification of cultivars with similar phenotypic traits. To prevent misidentification of olive cultivars and to minimize intra-cultivar variation, certified propagation materials which were characterized using DNA based molecular markers should be used during the establishment of new olive orchards.

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

“…Although the genetic basis of many morphological and agronomic traits has not been yet determined in olives, environmental influences on these characters can lead to misidentification of olive genotypes and cultivars. In addition, homonyms, synonyms and misnaming have been reported to be common in olive (Fendri et al, 2010;Ipek et al, 2012). Recently, different types of molecular markers have been used to reveal genetic diversity between olive genotypes.…”

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

“…A high number of studies dedicated at cultivar genotyping have been carried out recently, with the specific aim to solve these problems. When SSRs have been used for such purpose, they evidenced that some cultivar names refer to a polyclonal population (Bracci et al 2009;Ben-Ari et al 2014;Caruso et al 2014;Ipek et al 2012).…”

“…), Argentina, Colombia(Beghè et al 2015), Iran(Mardi et al 2016;Sorkheh and Khaleghi 2016;Noormohammadi et al 2014;Mousavi et al 2014), Palestine(Obaid et al 2014), Turkey(Sakar et al 2016a, b;Ipek et al 2012;Ercisli et al 2012;Işik et al 2011), Algeria (Abdessemed et al 2015; Dominguez-Garcia et al 2012), Spain (Fernández i Martí et al 2015; Delgado-Martinez et al 2012), Italy (Caruso et al 2014; Las Casas et al 2014; Marra et al 2013; Colao et al 2011; Rotondi et al 2011b; Corrado et al 2011), Greece (Linos et al 2014), Tunisia (Ben-Ayed et al 2014a, b; Abdelhamid et al 2013), Morocco (El Bakkali et al 2013a, b), Australia (Rehman et al 2012) and Croatia (Ercisli et al 2012). Independent research groups (Ben Ayed et al 2016; Trujillo et al 2014; Xanthopoulou et al 2014; Haouane et al 2011) have carried out analysis of the genetic relationships between the different accessions maintained in ex situ conservation germplasms, collecting large amounts of samples from all over the world.…”

Genetic Diversity and Relationships among Local Olive (<i>Olea europeaea</i> L.) Genotypes from Gaziantep Province and Notable Cultivars in Turkey, Based on SSR Markers

“…Morphological criteria such as leaf, fruit, seed and growth behaviour have been used to evaluate olive diversity, as well as to determine the origin of olive trees. An evaluation of phenotypic diversity was used to discriminate olive cultivars with distinct morphological and pomological characters (Ipek et al, 2012). There are many systematic identification procedures that have been developed to help identify genetic diversity in olive trees.…”