“…Seedlings with the second lanceolate leaf fully opened, stage 112 (Hormaza et al, 2012), were kept at field capacity for one month before being subjected to different soil water potentials in a 60-day period. The depth of irrigation to be applied in each water potential was calculated taking into account the effective root depth, physical characteristics of the soil (texture, apparent density, and moisture retention curve), and soil moisture was monitored daily with a SM-200 soil moisture sensor (DELTA-T, Cambridge, the United Kingdom) attached to the manual HH2 datalogger (DELTA-T, Cambridge, the United Kingdom).…”