Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

Abstract

Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt) was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt), identities varied from 16.8 to 28.6 %. The name Catharanthus mosaic virus (CatMV) is proposed for this new potyvirus.

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

“…(1998). The obtained RNA was used for virus detection by one‐step reverse transcription‐polymerase chain reaction (RT‐PCR) with the potyvirus universal primers WCIEN/PV1, which amplifies a fragment of 800 bp comprising part of the coat protein (CP) gene and the 3′ non‐translated region (Maciel et al., 2011). The obtained amplicons were sent for nucleotide sequencing, in the sense direction, at the Institute of Biotechnology (IBTEC) of the University of the State of São Paulo, Brazil.…”

Ocurrence of pepper yellow mosaic virus and cucumber mosaic virus on Capsicum chinense in the state of Amazonas, Brazil

“…Total RNA was extracted from eight symptomatic C. chinense plants using a PureLink Viral RNA/DNA kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer's recommendations. One-step RT-PCR was performed using universal primers PV1/SP6 and WCIEN-sense, for detection of potyviruses (Mackenzie et al 1998;Maciel et al 2011), and specific primers for the coat protein gene of cucumber mosaic virus (CMV) (Wylie et al 1993), which amplify fragments of 800 and 480 bp, respectively. Amplicons of the expected size for each virus were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, South Korea).…”

Identification and characterization of a new potyvirus infecting cucurbits

“…Also, the DAG motif, involved in aphid transmission, is present at the N-terminus of the CP [3]. Finally, other consensus motifs found in the CP were MVW-CIENGTSP, AFDF, and QMKAAAL [21,23].…”