Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Abstract

Coffee ringspot is a minor coffee disease caused by the nuclear type of Brevipalpus mite-transmitted virus, Coffee ringspot virus (CoRSV). Recently outbreaks of the disease in some growing regions of the state of Minas Gerais, Brazil, were registered with qualitative and quantitative yield losses. Coffea arabica was the only species registered as natural host. A survey was made on a germplasm collection of Coffea and related species kept at the Centro de Café "Alcides Carvalho", Instituto Agronômico, Campinas, state of São Paulo (SP), Brazil, to assess natural susceptibility of Coffee species, other than C. arabica and some interspecific hybrids of Coffea as well as other non-Coffea plant species to the Coffee ringspot virus (CoRSV). The following plants were found with ringspot symptoms on their leaves and/or fruits besides C. arabica L.: C. kapakata (IAC 4511), C. dewevrei cv. Excelsa, C. canephora cv. Robusta, hybrid derivative of the C. arabica × C. racemosa (IAC1195-5-6-2), C. arabica × C. dewerei (Piatã IAC 387), Híbrido de Timor CIFC 832/1 (derivative from a natural crossing between C. arabica × C. canephora) and C. racemosa. Also Psilanthus ebracteolatus, a species close to the genus Coffee was also found with ringspot lesions on their leaves. All these plants were also found infested by Brevipalpus mites identified as B. phoenicis. Infection of these plants by CoRSV was confirmed by the observation of characteristic cytopathic effects in the tissues of the lesion and by RT-PCR using a pair of primer specific for CoRSV. Only with C. racemosa RT-PCR failed to amplify the CoRSV genome. The susceptibility of P. ebracteolatus to CoRSV adds new dimension regarding its controversial taxonomic position

Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

“…To detect cytopathic effects caused by BTV-C and -N, lesioned tissues were processed, fixed in Karnovsky reagent, embedded in Spurr's epoxy resin and examined by TEM (Kitajima et al, 2001). Leaves were assayed by RT-PCR using specific primers for SvSRV (Ferreira et al, 2007), CoRSV (Kitajima et al, 2011) and ClCSV. Total RNA from independent plants inoculated with each virus was extracted using TRIZOL® Reagent (Thermo Scientific, WI, USA).…”

Orchid Fleck Virus Structural Proteins N and P Form Intranuclear Viroplasm-Like Structures in the Absence of Viral Infection

“…3 and 7). It is of particular interest whether a similar interaction between the N and P proteins can be observed for other candidate dichorhabdoviruses, including the citrus leprosis nuclear type, coffee ringspot, and Clerodendrum chlorotic spot viruses (54)(55)(56)(57).…”

Common Bean: Experimental Indicator Plant for Citrus leprosis virus C and Some Other Cytoplasmic-Type Brevipalpus-Transmitted Viruses

“…Its genome is bipartite (5 and 9 kb) and of positive-sense single-stranded (ss)RNA. Two other BrTV-Ns, CoRSV (17) and CICSV (26), have been partially sequenced, exhibiting sequence similarities with OFV, and may belong to the same genus as other, as-yet-uncharacterized BrTV-Ns. Parts of the genomes of PFGSV (1) and SvRSV (11) have been sequenced, showing sequence similarities with that of CiLV-C. LigRSV and HGSV (23) have not been molecularly characterized yet but their cytopathology indicates that they are BrTV-C.…”