2011
DOI: 10.1590/s0103-90162011000200016
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Abstract: The hybrid Eucalyptus grandis × Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two… Show more

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Cited by 14 publications
(14 citation statements)
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“…Standard and double MS concentrations of boron and calcium have both been used for organogenic culture of E. grandis [122], whereas CaCl 2 has been eliminated from N7 medium during callogenesis, but incorporated during shoot regeneration, of E. urophylla [86]. An MS medium with 4.90 mM NH 4 NO 3 , 5.68 mM K 2 SO 4 , 1 µM CuSO 4 ·5H 2 O, and no KNO 3 has been employed for organogenesis and subsequent shoot development of E. grandis × E. urophylla [123].…”
Section: Organogenesismentioning
confidence: 99%
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“…Standard and double MS concentrations of boron and calcium have both been used for organogenic culture of E. grandis [122], whereas CaCl 2 has been eliminated from N7 medium during callogenesis, but incorporated during shoot regeneration, of E. urophylla [86]. An MS medium with 4.90 mM NH 4 NO 3 , 5.68 mM K 2 SO 4 , 1 µM CuSO 4 ·5H 2 O, and no KNO 3 has been employed for organogenesis and subsequent shoot development of E. grandis × E. urophylla [123].…”
Section: Organogenesismentioning
confidence: 99%
“…Thidiazuron (TDZ) and 2-Cl-PBU (i.e., 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea and N-phenyl-N -[6-(2-chlorobenzothiazol)-yl] urea, respectively) are potent promotors of callus formation in eucalypts. Organogenesis can be induced using 0.89 µM BA with 0.91 µM TDZ, or 0.5 µM TDZ with 0.2 µM 2,4-D for E. globulus [127,128], 2 µM TDZ, or 0.23 µM TDZ with 0.05 µM NAA, or 3 µM TDZ with 0.1 µM NAA for E. grandis × E. urophylla [89,123,128], and 2.27 µM TDZ with 0.54 µM NAA, or 1.14 µM 2-Cl-PBU with 0.57 µM IAA for E. urophylla [89,111,112]. Picloram (i.e., 4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) has been used alone at 20.7 µM for E. urophylla organogenesis [86] or at 0.04 µM in combination with 2.25 µM BA for E. gunnii Hook.f.…”
Section: Organogenesismentioning
confidence: 99%
“…They were subsequently immersed in a 1% solution of fungicide (Benomyl ® ) for 20 min. and finally washed three times with deionized and autoclaved water (ALCÂNTARA et al, 2011). The seeds were germinated in a recipient glass (6.5 x 7.0 cm) containing 40 mL of MS medium (MURASHIGE; SKOOG, 1962) free of plant growth regulator.…”
Section: Asepsis and Standardization Of The Explantsmentioning
confidence: 99%
“…The vegetative propagation of Eucalyptus species, obtained by cutting (ALMEIDA et al, 2007 DÍAZ et al, 2009;HOAD;LEAKEY, 1995), mini-cutting (BORGES et al, 2011;BRONDANI et al, 2010;GOULART et al, 2010;SCHWAMBACH et al, 2008; or micro-cutting (NAKHOODA et al, 2011;NOURISSIER;MONTEUUIS, 2008) improved the uniformity of the forests, their productivity and the quality of forest products (CHEN et al, 2001;KELLISON, 2007), thereby fostering the progress of the forestry sector in recent decades. Furthermore, numerous techniques of in vitro cultivation have been developed to provide greater rooting, thus promoting the rejuvenation of selected plants with superior characteristics (DUTRA et al, 2009;NEHRA et al, 2005).…”
Section: Introductionmentioning
confidence: 99%
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