Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production

Abstract

Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluorescent lamps, photoperiod 16 h per day at 25°C) and dark. Leaf explants of Barringtonia racemosa were cultured on modified Murashige and Skoog (MS), Woody Plant Medium (WPM) and B5 media, supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). Optimal conditions for callus induction and maintenance under both dark and light were investigated, and growth and lycopene accumulation were evaluated. Among media with different concentrations of 2,4-D, fast growing, friable callus initiated within three weeks after culturing on WPM basal medium supplemented with 2.0 mg L -1 (weight per volume) of 2,4-D, whereas callus induction in explants cultured on all other media started only after five weeks. Calli were subcultured once every fortnight. Pale yellow and green calli developed under conditions of dark and light respectively were then selected for evaluation of their lycopene contents. An improved reversed phase of high performance liquid chromatography (HPLC) method was used for a selective chemical determination of the lycopene content. Light induced lycopene production; and likewise maximum lycopene level incubated in light was higher than those incubated in darkness. The best growth rates of callus and cell suspension were achieved in WPM and B5 media respectively. The production of lycopene was growth-dependent through analysis of growth and lycopene content of both callus and cell suspension cultures. Key words: Powderpuff tree, carotene, phytochemical, plant micropropagation, growth hormones Otimização de culturas de suspensões de calos e células de Barringtonia racemosa (família Lecythidaceae) para produção de licopeno RESUMO: O licopeno está presente numa série de frutas frescas e hortaliças principalmente na folhas de Barringtonia racemosa. A extração tradicional do licopeno tem sido empregada no lugar da fácil técnica de propagação como o processo de cultura de células de explantes de folhas. É nossa intenção demonstrar como o licopeno pode ser extraído através de cultura de tecido sob luz (iluminação com lâmpadas fluorescentes brancas de 8.200 lux, 16 h por dia a 25° C) e escuro. Explantes de folhas de Barringtonia racemosa foram cultivados em meio modificado de Murashige e Skoog (MS) para plantas lenhosas e meio B5, suplementado com diferentes concentrações de ácido 2,4-Diclorofenoxiacético (2,4-D). Condições ótimas para indução e manutenção de calos sob luz e escuro foram investigadas e avaliados o crescimento e acumulo de licopeno. Entre meios com diferentes concentrações de 2,4 -D, calos friáveis de crescimento rápido tiveram início em três semanas após serem cultivados em...

Ethnobotanical Profiles and Phytochemical Constituents of Barringtonia racemosa L. for Potential Scrutiny of Bioactive Compounds through Plant Biotechnology

“…In such discovery, they confirmed the isolation of bartogenic acid from the fruits of this plant species. A terpene compound classified under tetraterpenoid had been studied by Behbahani et al, 9 and her findings verified the presence lycopene in callus and cell suspension cultures of B. racemosa.…”

“…The earliest documented protocol for the establishment of callus culture was made in 2011. In such experiment, the researchers found that the fast growing and friable calli formation were initiated in the culture of leaf explant treated with 2.0 mg/l 2,4-D in WPM medium 9 . Later on, in 2013 another research had been documented in describing the protocol of callus culture establishment from leaf and endosperm explants of B. racemosa 10 .…”

“…In addition to that, the plant biotechnology approach had been incorporated in a very few studies which include the establishment of callus culture and cell suspension culture of the species [8][9][10][11] . Due to the scarcity of investigations in plant biotechnology-related areas conducted on B. racemosa, therefore, there is a great potential for the species to be further studied in such a field.…”

GENETIC DIVERSITY AND POPULATION STRUCTURE AMONG Grewia tenax GENOTYPES IN SUDAN USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

“…tenax fruits were considered for a long time as a simple, naturally available food and medication against iron-deficiency anemia and fatigue [7,8,2].Anemia, which is one of the top ten death-causing disorders in developing countries, not related only to malnutrition and poverty, but also to the free radicals activities [9,10]. Grewia tenax extracts were report to have effects on the regulation of iron digestive transfer and absorption [8].…”

“…1350 IJBPAS, July, 2018, 7(7) Group 4 contained three subgroups, the first and second were from Northern Kordofan (Abuhraz and Almnzfa), (2,5,16,7,8,14,12,17,18,15,10,13,9,35,36,37,38,3,4,6,11), the third from Northern Kordofan (Abuhraz, Almnzfa and Elobaid1), ( 3,4,6,11,21,22,34,23,24,33,25,27,26,28,29,30,32,31). In the present study, 14 of RAPD markers (Table 3) the studies in Grewia optiva species [37].…”